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Bupi Hewei decoction ameliorates 5-fluorouracil-induced intestinal dysbiosis in rats through t helper 17/t regulatory cell signaling pathway

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Journal of traditional Chinese Medicine 2020年第1期40卷 38-48页

作者： Sun Zhigao Hu Yazhuo Wang Yuguo Feng Jian Dou Yongqi Department of traditional Chinese Medicine Chinese PLA General HospitalBeijing 100853China&Department of Traditional Chinese MedicineHainan Branch of Chinese PLA General HospitalSanya 572013China Institute of Gerontology Chinese PLA General HospitalBeijing 100853China Department of traditional Chinese Medicine Chinese PLA General HospitalBeijing 100853China

OBJECtIVE:to observe the effects of the Bupi Hewei(BPHW)decoction on diarrhea and intestinal flora disorder induced by 5-fluorouracil(5-FU)and investigate the possible mechanism underlying these ***:thirty-five male Sprague-Dawley rats were randomly divided into four groups:normal control,5-FU,5-FU+BPHW decoction(10.5 g/kg for 5 consecutive days),and 5-FU+Bacillus licheni-formis capsule groups(0.2 g/kg for 5 consecutive days).Animal models were established via the intraperitoneal injection of 5-FU(30 mg/kg for 5 consecutive days).At the end of the treatment period,diarrhea was assessed,and the change of the intestinal flora was examined using 16 S r DNA highthroughput ***(IL)-17,IL-21,IL-6,IL-10,RAR-related orphan receptor gamma(RORγt),and forkhead box P3(Foxp3)expression in the jejunum was detected using immunohistochemistry,quantitative real-time polymerase chain reaction(PCR),Western blotting,and enzymelinked immuno sorbent ***:In this study,the BPHW decoction effectively lowered the diarrhea score,increased the proportions of Bacteroidetes and Prevotellaceae-Alloprevotella species,and reduced the proportions of Proteobacteria,Escherichia-Shigella,Ruminococcaceae NK4 A214,and Ruminococcaceae UCG-005 species in the rat intestine after 5-FU *** addition,the BPHW decoction significantly suppressed the expression of IL-17,IL-21,IL-6,IL-10,RORγt,and Foxp3 in the ***:Our findings suggest that the BPHW decoction can improve the intestinal immune balance and reduce intestinal inflammation by targeting t helper cell/t regulatory cell-associated factors.

关键词： Gastrointestinal microbiome Fluorouracil Drug therapy t-lymphocytes,regulatory th17 cells Bupi Hewei decoction

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Immunomodulatory effects of astragalus polysaccharide on Human peripheral blood mononuclear cells co-cultured with cervical cancer cell lineImmunomodulatory effects of astragalus polysaccharide on Human peripheral blood mononuclear cells co-cultured with cervical cancer cell line

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Journal of traditional Chinese Medicine 2021年第5期41卷 684-694页

作者： Elham Shokati Mohammad-Reza Shokri Kobra Entezami Samaneh Khorrami Mona Amani Morteza Motallebnezhad Elahe Safari Immunology Research Center Institute of Immunology and Infectious DiseasesIran University of Medical SciencesTehran ***Iran Department of Immunology Faculty of Medical SciencesIran University of Medical SciencesTehran ***Iran Department of Immunology School of Public HealthTehran University of Medical SciencesTehran ***Iran

OBJECtIVE:to investigate immunomodulatory effects of Astragalus polysaccharides(APS)on the co-culture of peripheral blood mononuclear cells(PBMCs)with He La cervical cancer cell ***:to assess the proliferation of PBMCs,carboxyfluorescein succinimidyl ester(CFSE)-labeled PBMCs were co-cultured with He La cells and treated with different concentrations of *** of cell culture were collected for cytokines assay via enzyme-linked immunosorbent assay(ELISA).the impact of APS on the proliferation of PBMCs,induction of regulatory t cells(tregs),and myeloid-derived suppressor cells(MDSCs)was carried out by flow ***:It was observed that APS could increase the proliferation of PBMCs co-cultured with He La cells(Pt effects on the induction of tregs and MDSCs in the co-culture assay(P>0.05).Furthermore,ELISA results demonstrated that APS could decrease IL-10 and tGF-βconcentration(Pthe above-mentioned characteristics showed that APS might be able to modulate immune responses and improve anti-tumor effects through increasing the proliferation of PBMCs and decreasing inhibitory cytokines secretion as critical mediators of immune suppression in the tumor microenvironment.

关键词： Astragalus polysaccharides uterine cervical neoplasms HeLa cells coculture techniques cytokines t-lymphocytes,regulatory

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Biological features of intrahepatic CD4^(+)CD25^(+)t cells in the naturally tolerance of rat liver transplantation

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Frontiers of Medicine 2007年第4期1卷 373-376页

作者： LU Ling ZHANG Feng PU Liyong YAO Aihua YU Yue SUN Beicheng LI Guoqiang WANG Xuehao Key Laboratory of Living Donor Liver transplantation of Ministry of Public Health Liver Transplantation CenterThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China

the biological features of intrahepatic CD4^(+)CD25^(+)t regulatory cells in the naturally tolerance of rat liver transplantation were *** liver transplan-tation was performed in two allogeneic rat strain combina-tions,one with fatal immunosuppression despite a complete major histocompatibility complex *** subjects were divided into three groups according to different donors and recipients[tolerance group:LEW-to-DA;Rejection group:DA-to-LEW;Syngegnic group(control group):DA-to-DA].the proportion of intrahepatic CD4^(+)CD25^(+)t cells from three groups was determined by flow cytometry(FCM)in different *** intrahepaitc CD4^(+)CD25^(+)t cells were isolated by magnetic activated cell sorting(MACS)method and identified by *** Foxp3 mRNA was detected by reverse transcriptase polymerase chain reaction(Rt-PCR).And their suppression on the proliferation of CD4^(+)CD25^(-)t effector cells was analyzed by cell proliferation assay in *** immediately after transplantation,the proportion of treg cells increased over time in both allogeneic groups but was significantly greater in the Rejection *** pro-portion of treg cells declined after day 5,and such reduction was more dramatic in the Rejection group than in the tole-rance *** in the tolerance group showed a second increase in the proportion after day *** CD4^(+)CD25^(+)t cells isolated from spontaneous tolerance models inhibited the proliferation of mixed lymphocyte *** purity of CD4^(+)CD25^(+)tcells sorted by MACS was 86%–93%.the CD4^(+)CD25^(+)t cells could specifically express the Foxp3 gene compared with CD4^(+)CD25^(-)t *** vitro,the spleen cells from LEW rats can irritate the proliferation of CD4^(+)CD25^(+)t cells more obviously than the syngegnic spleen ***4^(+)CD25^(+)tr cells could suppress the proliferation of CD4^(+)CD25^(-)t cells,but the inhibition was reversed by exo-genous IL-2(200 U/mL).the CD4^(+)CD25^(+)t regulatory

关键词： liver transplantation immune tolerance t-lymphocytes,regulatory CD4^(+)CD25^(+)t regulatory cells

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Changes of phenotype and function of human CD4^(+)CD25^(-) t cells induced by transfection of Foxp3

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Frontiers of Medicine 2008年第4期2卷 366-369页

作者： Kui WU Yutian BI Yaoli WANG Changzheng WANG Institute of Respiratory Disease Xinqiao HospitalThird Military Medical UniversityChongqing 400037China

the aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and function of naive CD4^(+)t *** pMSCV-Foxp3 retroviral vec-tor encoding Foxp3 gene was transduced into the Pt67 packaging cell ***-containing supernatant was applied to differentiate CD4^(+)CD25^(-) t *** resulting cells were sorted with flow *** expressions of CD25,CD127,CtLA-4 and the proliferation of trans-fected t cells were *** effect of transfected CD4^(+)t cells on the proliferation and cytokine production of CD4^(+)CD25^(-) t cells was ***3-gene trans-fected CD4^(+)t cells could express Foxp3 and transfection of Foxp3 gene up-regulated the expressions of CD25 and CtLA-4,but down-regulated CD127 *** transfection,the proliferation of CD4^(+)t cells was *** t cells inhibited the proliferation of CD4^(+)CD25^(-) t ***4^(+)CD25^(-) t cells acquired a reg-ulatory phenotype and function after it was transduced with the Foxp3 *** suggested a key role of Foxp3 in the generation of CD4^(+)CD25^(+) regulatory t cells.

关键词： Forkhead transcription factors FOXP3 pro-tein,human t-lymphocytes,regulatory Retroviridae

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Homoharringtonine promotes heart allograft acceptance by enhancing regulatory t cells induction in a mouse model

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Chinese Medical Journal 2024年第12期137卷 1453-1464页

作者： Xia Qiu Hedong Zhang Zhouqi tang Yuxi Fan Wenjia Yuan Chen Feng Chao Chen Pengcheng Cui Yan Cui Zhongquan Qi tengfang Li Yuexing Zhu Liming Xie Fenghua Peng tuo Deng Xin Jiang Longkai Peng Helong Dai Medical College Guangxi UniversityNanningGuangxi 530004China Department of Kidney transplantation Center of Organ TransplantationThe Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China National Clinical Research Center for Metabolic Diseases and Department of Metabolism and EndocrinologyThe Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China Department of Organ transplantation The Fifth Clinical Medical College of Henan University of Chinese Medicine(Zhengzhou People’s Hospital)ZhengzhouHenan 450000China

Background:Homoharringtonine(HHt)is an effective anti-inflammatory,anti-viral,and anti-tumor protein synthesis inhibitor that has been applied ***,we explored the therapeutic effects of HHt in a mouse heart transplant ***:Healthy C57BL/6 mice were used to observe the toxicity of HHt in the liver,kidney,and hematology.A mouse heart transplantation model was constructed,and the potential mechanism of HHt prolonging allograft survival was evaluated using Kaplan-Meier analysis,immunostaining,and bulk RNA sequencing *** HHt-t cell crosstalk was modeled ex vivo to further verify the molecular mechanism of HHt-induced regulatory t cells(tregs)***:HHt inhibited the activation and proliferation of t cells and promoted their apoptosis ex *** of 0.5 mg/kg HHt for 10 days significantly prolonged the mean graft survival time of the allografts from 7 days to 48 days(Pthout non-immune *** allografts had long-term survival after continuous HHt treatment for 28 *** significantly reduced lymphocyte infiltration in the graft,and interferon-γ-secreting CD4^(+)and CD8^(+)t cells in the spleen(Pt significantly increased the number of peripheral tregs(about 20%,Pterleukin(IL)-10 *** downregulated the expression of t cell receptor(tCR)signaling pathway-related genes(CD4,H2-Eb1,tRAt1,and CD74)and upregulated the expression of IL-10 and transforming growth factor(tGF)-βpathway-related genes and treg signature genes(CtLA4,Foxp3,CD74,and ICOS).HHt increased CD4^(+)Foxp3^(+)cells and Foxp3 expression ex vivo,and it enhanced the inhibitory function of inducible ***:HHt promotes treg cell differentiation and enhances treg suppressive function by attenuating the tCR signaling pathway and upregulating the expression of treg signature genes and IL-10 levels,thereby promoting mouse heart allograft *** findings may have therapeutic implications for organ tr

关键词： Heart transplantation Homoharringtonine Immunosuppressive agents t-lymphocytes,regulatory Graft acceptance

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Effect of Jiaweiwumei decoction on regulatory t cells and interleukin-10 in a rat model of ulcerative colitis

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Journal of traditional Chinese Medicine 2015年第3期35卷 312-315页

作者： tao Mei Wang Xuyan Wang Aiqin Gao Ninghan Pan Jie Liu Xiaoquan Wang Xiaoye Department of Digestive System the Ninth Hospital of Xi'an Department of Endocrinology and Genetic Metabolic Xi'an Children's Hospital Pediatric Intensive Care Unit Xi'an Children's Hospital

OBJECtIVE:to investigate the effect of a decoction made from the traditional Chinese Medicine Wumei pill,on regulatory t cells and interleukin-10(IL-10) in a rat model of ulcerative colitis induced by2,4,6-trinitrobenzene sulfonic acid(tNBS).MEtHODS:Rat ulcerative colitis was induced with *** modeled rats were randomly divided into six groups:normal control group;model group;sulfasalazine suppositories treatment group;and high,moderate,and low dosage of Jiaweiwumei decoction groups(12 rats each).Colon injury index was evaluated after 14 *** peripheral blood lymphocyte separation,CD4 + t cells and CD4+/CD25+ t cell percentage was detected by flow *** content of IL-10 in serum and intestinal mucosa tissue was detected by sandwich enzyme-linked immunosorbent ***:Colon injury indices in the decoction groups were effectively reduced,compared with the model group(P th that of the control group,the CD4+/CD25+ to CD4+ t lymphocyte ratio of the model group was significantly lower,while the decoction treatment improved the CD4 +/CD25 + to CD4 + t lymphocyte ratio(P the serum and mucosal IL-10 content of the model group was significantly lower(P than that in the control group,while the decoction group had significantly higher serum and intestinal mucosal IL-10 content than that in the model group(P the regulatory t cell content was negatively correlated with the colonic injury index(r = 0.68,P tively correlated with the content of serum IL-10(r = 0.87,P testinal mucosal IL-10(r= 0.79,P tion had significant effects on regulatory t cells and IL-10 in rats with tNBS-induced ulcerative colitis.

关键词： Colitis,ulcerative t-lymphocytes,regulatory Interleukin-10 Jiaweiwumei decoction

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