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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第6期9卷 455-463页

作者： Jing-hong CHEN Jun-ling CAO Yong-lie CHU Zhi-lun WANG Zhan-tian YANG Hong-lin WANG Ministry of Education Key Laboratory of Environment and Genes related to Diseases Institute of Endemic Diseases School of Medicine Xi ＇an Jiaotong University Xi ＇an 710061 China

Objective:to investigate the effects of t-2 toxin on expressions of Fas,p53,Bcl-xL,Bcl-2,Bax and caspase-3 on human ***:Human chondrocytes were treated with t-2 toxin(1～20 ng/ml)for 5 ***,p53 and other apoptosis-related proteins such as Bax,Bcl-2,Bcl-xL,caspase-3 were determined by Western blot analysis and their mRNA expressions were determined by reverse transcriptase-polymerase chain reaction(Rt-PCR).Results:Increases in Fas,p53 and the pro-apoptotic factor Bax protein and mRNA expressions and a decrease of the anti-apoptotic factor Bcl-xL were observed in a dose-dependent manner after exposures to 1～20 ng/ml t-2 toxin,while the expression of the anti-apoptotic factor Bcl-2 was ***,t-2 toxin could also up-regulate the expressions of both pro-caspase-3 and caspase-3 in a dose-dependent ***:these data suggest a possible underlying molecular mechanism for t-2 toxin to induce the apoptosis sig- naling pathway in human chondrocytes by regulation of apoptosis-related proteins.

关键词： Apoptosis Apoptosis-related proteins Chondrocyte t-2 toxin

Promotion of the articular cartilage proteoglycan degradation by t-2 toxin and selenium protective effect

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第1期9卷 22-33页

作者： Si-yuan LI Jun-ling CAO Zhong-li SHI Jing-hong CHEN Zeng-tie ZHANG Clare E. HUGHES Bruce CAtERSON Institute of Endemic Diseases College of Medicine Xi＇an Jiaotong University Key Laboratory of Environment and Genes Related to Diseases （Xi＇an Jiaotong University） Ministry of Education Kev Laboratory of Microelement and Endemic Disease （Xi＇an Jiaotong University） Ministr） of Health Xi＇an 710061 China Laboratory of Connective tissue Biology School of Biosciences Cardiff University Cardiff CF10 3US UK

Objective: to identify the relationship between t-2 toxin and Kashin-Beck disease (KBD),the effects of t-2 toxin on aggrecan metabolism in human chondrocytes and cartilage were investigated in vitro. Methods: Chondrocytes were isolated from human articular cartilage and cultured in vitro. Hyaluronic acid (HA),soluble CD44 (sCD44),IL-1β and tNF-α levels in super-natants were measured by enzyme-linked immunosorbent assay (ELISA). CD44 content in chondrocyte membrane was deter-mined by flow cytometry (FCM). CD44,hyaluronic acid synthetase-2 (HAS-2) and aggrecanases mRNA levels in chondrocytes were determined using reverse transcription polymerase chain reaction (Rt-PCR). Immunocytochemical method was used to investigate expressions of BC-13,3-B-3(-) and 2-B-6 epitopes in the cartilage reconstructed in vitro. Results: t-2 toxin inhibited CD44,HAS-2,and aggrecan mRNA expressions,but promoted aggrecanase-2 mRNA expression. Meanwhile,CD44 expression was found to be the lowest in the chondrocytes cultured with t-2 toxin and the highest in control plus selenium group. In addition,ELISA results indicated that there were higher sCD44,IL-1β and tNF-α levels in t-2 toxin group. Similarly,higher HA levels were also observed in t-2 toxin group using radioimmunoprecipitation assay (RIPA). Furthermore,using monoclonal antibodies BC-13,3-B-3 and 2-B-6,strong positive immunostaining was found in the reconstructed cartilage cultured with t-2 toxin,whereas no positive staining or very weak staining was observed in the cartilage cultured without t-2 toxin. Selenium could partly inhibit the effects of t-2 toxin above. Conclusion: t-2 toxin could inhibit aggrecan synthesis,promote aggrecanases and pro-inflammatory cytokines production,and consequently induce aggrecan degradation in chondrocytes. these will perturb metabolism balance between aggrecan synthesis and degradation in cartilage,inducing aggrecan loss in the end,which may be the initiation of the cartilage degradation.

关键词： t-2 toxin Kashin-Beck disease (KBD) Aggrecan IL-1β tNF-α Aggrecanase Hyaluronic acid (HA) CD44

Effective protective agents against the organ toxicity of t-2 toxin and corresponding detoxification mechanisms:A narrative review

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Animal Nutrition 2024年第1期16卷 251-266页

作者： Pengju Wang Lv-hui Sun Xu Wang Qinghua Wu Aimei Liu Hubei Key Laboratory of Diabetes and Angiopathy Medicine Research InstituteXianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Hubei Hongshan Laboratory College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan430070China National Reference Laboratory of Veterinary Drug Residues(HZAU)and MAO Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural UniversityWuhan430070China College of Life Science Yangtze UniversityJingzhou 434025China

t-2 toxin is one of the most widespread and toxic fungal toxins in food and *** can cause gastrointestinal toxicity,hepatotoxicity,immunotoxicity,reproductive toxicity,neurotoxicity,and nephrotoxicity in humans and animals.t-2 toxin is physicochemically stable and does not readily degrade during food and feed ***,suppressing t-2 toxin-induced organ toxicity through antidotes is an urgent *** agents against the organ toxicity of t-2 toxin have been recorded widely in the literature,but these protective agents and their molecular mechanisms of detoxification have not been comprehensively *** this review,we provide an overview of the various protective agents to t-2 toxin and the molecular mechanisms underlying the detoxification *** appropriate targets to antagonize t-2 toxin toxicity is also an important *** review will provide essential guidance and strategies for the better application and development of t-2 toxin antidotes specific for organ toxicity in the future.

关键词： t-2 toxin Organ toxicity Protective agent Detoxification mechanism

Effects of t-2 toxin Exposure on Bone Metabolism and Bone Development of Mice

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Journal of Northeast Agricultural University(English Edition) 2022年第1期29卷 89-96页

作者： Cui Yi-long Cao Zheng Zhang Jian Song Miao Li Yan-fei College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150030China

t-2 toxin is the most widespread mycotoxin in crops,feed and food,which poses a serious threat to body *** is the main target tissue for t-2 toxin *** of food contaminated by t-2 toxin is the main cause of Kashin-Beck ***,the specific mechanism of bone damage caused by t-2 toxin is still *** this study,a total of 40 male C57BL/6N mice were divided into four groups and orally treated with 0,0.5,1.0 and 2.0 mg·kg^(-1) body weight t-2 toxin for 28 *** results showed that exposure to t-2 toxin led to weight loss,bone mineral density reduction and femoral structural damage of *** addition,osteoblast-mediated bone formation was inhibited,and osteoclast-mediated bone resorption was ***,the levels of bone metabolism-related hormones including parathyroid hormone,calcitonin and 1,25-dihydroxyvitamin D3 were *** importantly,it was found that the level of neuropeptide Y(a neurohormone)was *** results provided a new perspetive for understanding the osteotoxicity of t-2 toxin.

关键词： t-2 toxin bone metabolism parathyroid hormone calcitonin 1,25-dihydroxyvitamin D3 neuropeptide Y

Real-time in situ observation of P53-mediated cascade activation of apoptotic pathways with nucleic acid multicolor fluorescent probes based on symmetrical gold nanostars

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Nano Research 2023年第4期16卷 5391-5400页

作者： Chenbiao Li Peifang Chen Xiaoyuan Ma Xichi Lin Shan Xu Sobia Niazi Zhouping Wang State Key Laboratory of Food Science and technology Jiangnan UniversityWuxi 214122China School of Food Science and technology Jiangnan UniversityWuxi 214122China International Joint Laboratory on Food Safety Jiangnan UniversityWuxi 214122China Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxi 214122China

t-2 toxin,one of the most dangerous natural pollutants,induces apoptosis through multiple ***,P53 mediated apoptosis pathway,an important collection of molecules,plays a key role in cell vital ***-time monitoring of upstream and downstream activation relationships of P53 mRNA,Bax mRNA,and cytochrome c(Cyt c)in signaling pathways is of great significance for understanding the apoptotic machinery in human *** this work,a novel nucleic acid multicolor fluorescent probe,based on silica-coated symmetric gold nanostars(S-AuNSs@SiO_(2)),was developed for highly sensitive in situ real-time imaging of P53 mRNA,Bax mRNA,and Cyt c during t-2 toxin-induced *** nucleic acid chains modified with carboxyl groups were modified on the surface of S-AuNSs@SiO_(2)by amide *** complementary chains of targeted mRNA and the aptamer of targeted Cyt c were modified with different fluorophores,respectively,and successfully hybridized on S-AuNSs@SiO_(2)*** targets were present,the fluorescent chains bound to the targets and detached from the material,resulting in the quenched fluorescence being *** probes based on S-AuNSs showed excellent performance is partly ascribed to the presence of 20 symmetric“hot spots”.Notably,the amide-bonded probe exhibited excellent anti-interference capability against biological agents(nucleases and biothiols).During the real-time fluorescence imaging of t-2 toxin-induced apoptosis,the corresponding fluorescence signals of P53 mRNA,Bax mRNA,and Cyt c were observed ***,S-AuNSs@SiO_(2)probe not only provides a novel tool for real-time monitoring of apoptosis pathways cascade but also has considerable potential in disease diagnosis and pharmaceutical medical.

关键词： nucleic acid multicolor fluorescent probe symmetric gold nanostars t-2 toxin P53 mediated apoptosis pathway living cells imaging

tHE EXPERIMENtAL StUDY ON tHE EFFECtS OF SIX MYCOtoxinS ON tHE CULtURAL CHONDROCYtES

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Journal of Pharmaceutical Analysis 1998年第1期12卷 1-8页

作者：曹峻岭熊咏民李斯纯郑滨张矢远毕华银莫东旭 Institute of Endemic Bone Diseases Xi'an Medical University Xi'an 710061 China

the effects of deoxynlvalenol (DON), t--2 toxin, nivalenol (NIv), hutenolide (But).alternariol methyl ether(AME) and monlliformin (cON ) on rabbit articular chondrocytes were observed by using the method of chondrocyte monolayer culture. the amounts or DNA in chondrocytesand glucuronate in matrix were measured. And the chondrocytes were observed by inversion microscope and transmission electron microscope (tEM). the results showed that the cultured chondrocytes were damaged by all the six mycotoxinsl and the synthesis of DNA and the divided reproductionof chondrocytes were restrained; the damage errect was more evident, esl,ecially in the early stage ofculturel the higher concentration or toxin in the media was used, the lower density of the culturalckondrocytes was observed; the cells were even round damaged and dead, so long as the media contolued toxin. When the six mycotoxins arrected the cultural chondrocytes ***, three dirfereut kinds or ultrastructural changes in ckondrocytes were seen by tEa. the relationship betweenmycotoxiu and KBD was preliminarily discussed, and some problems still need further investigation..

关键词： chondrocyte culture deoxynivalenol t-2 toxin nivalenol butenolide alternariol methyl ether moniliformin Kashin-Beck disease