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Neurotoxic role of interleukin-17 in neural stem cell differentiation after intracerebral hemorrhage

Neural Regeneration Research 2020年第7期15卷 1350-1359页

作者： Lu Gao Ping-Ping Li tian-Yu Shao Xiang Mao Hao Qi Bing-Shan Wu Ming Shan Lei Ye Hong-Wei Cheng Department of Neurosurgery First Affiliated Hospital of Anhui Medical University

Interleukin 17(IL-17)and its main producer,t cell receptorγδcells,have neurotoxic effects in the pathogenesis of intracerebral hemorrhage(ICH),aggravating brain *** investigate the correlation between IL-17 and ICH,we dynamically screened serum IL-17 concentrations using enzyme-linked immunosorbent assay and explored the clinical values of IL-17 in ICH *** was a significant negative correlation between serum IL-17 level and neurological recovery status in ICH patients(r=–0.498,Pto study the neurotoxic role of IL-17,C57 BL/6 mice were used to establish an ICH model by injecting autologous blood into the caudate ***,the mice were treated with mouse neural stem cells(NSCs)and/or IL-17 neutralizing antibody for 72 *** cytometry,brain water content detection,Nissl staining,and terminal deoxynucleotidyl transferase-mediated dUtP nick end labeling results indicated that NSC transplantation significantly reduced IL-17 expression in peri-hematoma tissue,but there was no difference in t cell receptorγδ*** with the ICH group,there were fewer apoptotic bodies and more Nissl bodies in the ICH+NSC group and the ICH+NSC+IL-17 *** investigate the potential effect of IL-17 on directional differentiation of NSCs,we cultured mouse NSCs(NE-4 C)alone or co-cultured them with t cell receptorγδcells,which were isolated from mouse peripheral blood mononuclear cells,for 7 *** results of western blot assays revealed that IL-17 secreted by t cell receptorγδcells reduced the differentiation of NSCs into astrocytes and neurons,while IL-17 neutralization relieved the inhibition of directional differentiation into astrocytes rather than *** conclusion,serum IL-17 levels were elevated in the early stage of ICH and were negatively correlated with outcome in ICH *** experiments and cytological investigations therefore demonstrated that IL-17 probably has neurotoxic roles in ICH because of its inhibitory effects

关键词： antibody neutralization astrocytes directional differentiation interleukin 17 intracerebral hemorrhage neural stem cells Nissl staining recovery t cell receptorγδcells tUNEL staining

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