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Coxsackievirus B3 HFMD animal models in syrian hamster and rhesus monkey

Virologica Sinica 2024年第2期39卷 290-300页

作者： Suqin Duan Wei Zhang Yongjie Li Yanyan Li Yuan Zhao Weihua Jin Quan Liu Mingxue Li Wenting Sun Lixiong Chen Hongjie Xu Jie Tang Jinghan Hou Zijun Deng Fengmei Yang Shaohui Ma Zhanlong He Institute of Medical Biology Chinese Academy of Medical Sciences Medical Primate Research Center Peking Union Medical CollegeYunnan Key Laboratory of Vaccine Research Development on Severe Infectious DiseaseKunming650118China

Coxsackievirus B3(CVB3)is the pathogen causing hand,foot and mouth disease(HFMD),which manifests across a spectrum of clinical severity from mild to ***,CVB3-infected mouse models mainly demonstrate viral myocarditis and pancreatitis,failing to replicate human HFMD *** several enteroviruses have been evaluated in syrian hamsters and rhesus monkeys,there is no comprehensive data on *** this study,we have first tested the susceptibility of syrian hamsters to CVB3 infection via different *** results showed that syrian hamsters were successfully infected with CVB3 by intraperitoneal injection or nasal drip,leading to nasopharyngeal colonization,acute severe pathological injury,and typical HFMD ***,the nasal drip group exhibited a longer viral excretion cycle and more severe pathological *** the subsequent study,rhesus monkeys infected with CVB3 through nasal drips also presented signs of HFMD symptoms,viral excretion,serum antibody conversion,viral nucleic acids and antigens,and the specific organ damages,particularly in the ***,there were no significant differences in myocardial enzyme levels,and the clinical symptoms resembled those often associated with common,mild *** summary,the study successfully developed severe syrian hamsters and mild rhesus monkey models for CVB3-induced *** models could serve as a basis for understanding the disease pathogenesis,conducting pre-trial prevention and evaluation,and implementing post-exposure intervention.

关键词： Coxsackievirus B3(CVB3) Hand foot and mouth disease(HFMD) Animal models syrian hamster Rhesus monkey

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Identification of optimal reference genes in golden syrian hamster with ethanol-and palmitoleic acid-induced acute pancreatitis using quantitative real-time polymerase chain reaction

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Animal Models and Experimental Medicine 2023年第6期6卷 609-618页

作者： Jinxin Miao Le Kang Tianfeng Lan Jianyao Wang Siqing Wu Yifan Jia Xia Xue Haoran Guo Pengju Wang Yan Li Academy of Chinese Medicine Science Henan University of Chinese MedicineZhengzhouChina Sino-British Research Centre for Molecular Oncology National Center for International Research in Cell and Gene TherapySchool of Basic Medical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhouChina Henan Key Laboratory of Helicobacter pylori and Microbiota and Gastrointestinal Cancer Marshall Medical Research CenterThe Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

Background:Acute pancreatitis(AP)is a severe disorder that leads to high morbidity and *** reference genes are important for gene analysis in *** study sought to study the expression stability of several reference genes in the golden syrian hamster,a model of ***:AP was induced in golden syrian hamster by intraperitoneal injection of ethanol(1.35 g/kg)and palmitoleic acid(2 mg/kg).The expression of candidate genes,including Actb,Gapdh,Eef2,Ywhaz,Rps18,Hprt1,Tubb,Rpl13a,Nono,and B2m,in hamster pancreas at different time points(1,3,6,9,and 24 h)posttreatment was analyzed using quantitative polymerase chain *** expression stability of these genes was calculated using Best Keeper,Comprehensive Delta CT,Norm Finder,and ge Norm algorithms and Ref Finder ***:Our results show that the expression of these reference genes fluctuated during AP,of which Ywhaz and Gapdh were the most stable genes,whereas Tubb,Eef2,and Actb were the least stable ***,these genes were used to normalize the expression of TNF-αmessenger ribonucleic acid in inflamed ***:In conclusion,Ywhaz and Gapdh were suitable reference genes for gene expression analysis in AP induced in syrian hamster.

关键词： acute pancreatitis reference genes syrian hamster TNF-α

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Comparative studies between humans and golden syrian hamsters via thromboelastography

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Animal Models and Experimental Medicine 2024年第4期7卷 570-577页

作者： Ze Yang Lili Xie Jingjing Ba Simin Zan Letong Zhang Xinyi Zhang Yang Yu School of Laboratory Animal&Shandong Laboratory Animal Center Shandong First Medical University&Shandong Academy of Medical SciencesJinanChina The Second Affiliated Hospital of Shandong First Medical University TaianChina

Background:Thromboelastography(TEG)is a widely utilized clinical testing method for real-time monitoring of platelet function and the thrombosis *** metab-olism disorders are crucial risk factors for *** lipid metabolism charac-teristics of hamsters resemble those of humans more closely than mice and rats,and their relatively large blood volume makes them suitable for studying the mechanisms of thrombosis related to plasma lipid *** blood samples from golden syrian hamsters and healthy humans were obtained following standard clinical *** was employed to evaluate coagulation factor function,fibrinogen(Fib)function,platelet function,and the fibrinolytic ***:The whole blood from hamster or healthy human was isolated following the clinical procedure,and TEG was employed to evaluate the coagulation factor func-tion,Fib function,platelet function,and fibrinolytic *** analysis used ACLTOP750 automatic coagulation analysis *** routine testing used XN-2000 automatic blood ***:TEG parameters revealed that hamsters exhibited stronger coagulation fac-tor function than humans(reaction time[R],p=0.0117),with stronger Fib function(alpha angle,phamsters displayed higher coagulation status than humans(coagulation index[CI],p=0.0023),and the rate of blood clot dissolution in hamsters differed from that in humans(percentage lysis 30 min after MA,p=0.02).Coagulation analysis parameters indicated that prothrombin time(PT)and activated partial thromboplastin time(APTT)were faster in hamsters than in hu-mans(PT,p=0.0014;APTT,p=0.03),whereas the Fib content was significantly lower in hamsters than in humans(p<0.0001).No significant difference was observed in thrombin time(p=0.1949).Conclusions:In summary,TEG could be used to evaluate thrombosis and bleeding parameters in whole blood samples

关键词： platelet function syrian hamster thromboelastography(TEG) thrombosis

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Establishment and application of a surrogate model for human Ebola virus disease in BSL-2 laboratory

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Virologica Sinica 2024年第3期39卷 434-446页

作者： Wanying Yang Wujian Li Wujie Zhou Shen Wang Weiqi Wang Zhenshan Wang Na Feng Tiecheng Wang Ying Xie Yongkun Zhao Feihu Yan Xianzhu Xia Hebei Key Lab of Laboratory Animal Science Department of Laboratory Animal ScienceHebei Medical UniversityShijiazhuang050017China Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research InstituteChinese Academy of Agricultural SciencesChangchun130122China College of Veterinary Medicine Jilin UniversityChangchun130062China College of Veterinary Medicine Jilin Agricultural UniversityChangchun130118China

The Ebola virus(EBOV)is a member of the Orthoebolavirus genus,Filoviridae family,which causes severe hemorrhagic diseases in humans and non-human primates(NHPs),with a case fatality rate of up to 90%.The development of countermeasures against EBOV has been hindered by the lack of ideal animal models,as EBOV requires handling in biosafety level(BSL)-4 ***,accessible and convenient animal models are urgently needed to promote prophylactic and therapeutic approaches against *** this study,a recombinant vesicular stomatitis virus expressing Ebola virus glycoprotein(VSV-EBOV/GP)was constructed and applied as a surrogate virus,establishing a lethal infection in *** infection with VSV-EBOV/GP,3-week-old female syrian hamsters exhibited disease signs such as weight loss,multi-organ failure,severe uveitis,high viral loads,and developed severe systemic diseases similar to those observed in human EBOV *** animals succumbed at 2–3 days post-infection(dpi).Histopathological changes indicated that VSV-EBOV/GP targeted liver cells,suggesting that the tissue tropism of VSV-EBOV/GP was comparable to wild-type EBOV(WT EBOV).Notably,the pathogenicity of the VSV-EBOV/GP was found to be species-specific,age-related,gender-associated,and challenge ***,equine anti-EBOV immunoglobulins and a subunit vaccine were validated using this ***,this surrogate model represents a safe,effective,and economical tool for rapid preclinical evaluation of medical countermeasures against EBOV under BSL-2 conditions,which would accelerate technological advances and breakthroughs in confronting Ebola virus disease.

关键词： Ebola virus(EBOV) Recombinant vesicular stomatitis virus Pathogenicity syrian hamster Surrogate models Vaccine evaluation and drug screening

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Promising xenograft animal model recapitulating the features of human pancreatic cancer

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World Journal of Gastroenterology 2020年第32期26卷 4802-4816页

作者： Jin-Xin Miao Jian-Yao Wang Hao-Ze Li Hao-Ran Guo Louisa S Chard Dunmall Zhong-Xian Zhang Zhen-Guo Cheng Dong-Ling Gao Jian-Zeng Dong Zhong-De Wang Yao-He Wang Sino-British Research Centre for Molecular Oncology National Centre for International Research in Cell and Gene TherapyAcademy of Medical SciencesZhengzhou UniversityZhengzhou 450000Henan ProvinceChina Academy of Chinese Medical Sciences Henan University of Chinese MedicineZhengzhou 450000Henan ProvinceChina Centre for Biomarkers and Biotherapeutics Barts Cancer InstituteQueen Mary University of LondonLondon EC1M6BQUnited Kingdom Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China Department of Animal Dairy and Veterinary SciencesUtah State UniversityLogan UT 84341United States

BACKGROUND Multiple sites of metastasis and desmoplastic reactions in the stroma are key features of human pancreatic cancer(PC).There are currently no simple and reliable animal models that can mimic these features for accurate disease *** To create a new xenograft animal model that can faithfully recapitulate the features of human *** Interleukin 2 receptor subunit gamma(IL2RG)gene knockout syrian hamster was created and characterized.A panel of human PC cell lines were transplanted into IL2RG knockout syrian hamsters and severe immune-deficient mice subcutaneously or *** growth,local invasion,remote organ metastasis,histopathology,and molecular alterations of tumor cells and stroma were compared over *** The syrian hamster with IL2RG gene knockout(named ZZU001)demonstrated an immune-deficient phenotype and ***001 hamsters faithfully recapitulated most features of human PC,in particular,they developed metastasis at multiple *** tissues derived from ZZU001 hamsters displayed desmoplastic reactions in the stroma and epithelial to mesenchymal transition phenotypes,whereas PC tissues derived from immune-deficient mice did not present such *** ZZU001 hamsters engrafted with human PC cells are a superior animal model compared to immune-deficient ***001 hamsters can be a valuable animal model for better understanding the molecular mechanism of tumorigenesis and metastasis and the evaluation of new drugs targeting human PC.

关键词： Pancreatic cancer Xenotrans plantation syrian hamster IL-2 receptor gamma chain gene Metastasis Animal model

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