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Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection

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Synthetic and Systems Biotechnology 2021年第4期6卷 283-291页

作者： Xiangxiang Zhao Zhengduo Wang Bowen Yang Zilong Li Yaojun Tong Yuhai Bi Zhenghong Li Xuekui Xia Xiangyin Chen Lixin Zhang Weishan Wang Gao-Yi Tan State Key Laboratory of Bioreactor Engineering and School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai200237China State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China State Key Laboratory of Microbial Metabolism and School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early-warning(CASCIRE)CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID)Chinese Academy of SciencesBeijing100101China Key Biosensor Laboratory of Shandong Province Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Jinan250013China

Antigen detection provides particularly valuable information for medical diagnoses;however,the current detection methods are less sensitive and accurate than nucleic acid *** combination of CRISPR/Cas12a and aptamers provides a new detection paradigm,but sensitive sensing and stable amplification in antigen detection remain ***,we present a PCR-free multiple trigger dsDNA tandem-based signal amplification strategy and a de novo designed dual aptamer synergistic sensing *** of these two strategies endowed the CRISPR/Cas12a and aptamer-based method with ultra-sensitive,fast,and stable antigen *** a demonstration of this method,the limit of detection was at the single virus level(0.17 fM,approximately two copies/μL)in SARS-CoV-2 antigen nucleocapsid protein analysis of saliva or serum *** entire procedure required only 20 *** our system’s simplicity and modular setup,we believe that it could be adapted reasonably easily for general applications in CRISPR/Cas12a-aptamer-based detection.

关键词： CRISPR/Cas12a Aptamer Antigen detection SARS-CoV-2 PCR-Free amplification synergistic sensing

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Electromechanical coupling properties of a self-powered vibration sensing device for near-surface observation tower monitoring

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Science China(Technological Sciences) 2022年第7期65卷 1545-1557页

作者： MU JiLiang HE HuiCheng MU JinBiao HE Jian SONG JinSha HAN XiaoTao FENG ChengPeng ZOU Jie YU JunBin CHOU XiuJian Science and Technology on Electronic Test and Measurement Laboratory North University of ChinaTaiyuan 030051China Sichuan Aerospace Fenghuo Servo Control Technology Corporation Chengdu 610000China

The wind-induced vibration of a remote sensing tower is the key factor affecting the stability of image sensing and structural reliability. Monitoring the vibration of a long-time unattended tower is critical to its proper operation. Currently, most monitoring devices are supplied with wired power or battery, significantly limiting their practical applications in remote areas. In this paper,a self-powered vibration sensing device based on hybrid electromechanical conversion mechanisms is proposed. The device depends on a cylindrical magnetic levitation structure sensitive to ambient vibration for transferring mechanical energy and is taken as a dual-functional heterogeneous integrated system comprising electromagnetic, piezoelectric, and triboelectric generators. When the device vibrates under environmental force driving, the suspension magnet reciprocates vertically and generates induced electromagnetic energy, which is used to power the device. Moreover, the triboelectric and piezoelectric voltages,respectively originating from magnet impact on two separation friction materials and magnetic field repulsion-induced strain deformation of a piezoelectric sheet, are used as the synergistic sensing signals. To improve the output energy, a set of dualsegmented annular coils is designed in an electromagnetic generator, which greatly avoids the obstructive effect of the suspended magnet on the magnetic flux change at its end. Compared with a whole isochoric coil, it increases the output voltage by 78.3%.For the triboelectric sensing module, a silicone film with a large specific surface area is fabricated via 3D modification, which improves the output voltage by 29.4%. Furthermore, a pair of piezoelectric sensing modules is set to improve the accuracy of comparative sensing data. The experimental measurement shows that the device maintains a high sensitivity of 6.711 V(m s;);and excellent linearity of 0.991 in the range of 0–14 m s;. This work provides a practical strategy

关键词： remote sensing tower electromechanical coupling synergistic sensing magnetic levitation vibration online monitoring

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