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Neurogranin as an important regulator in swimming training to improve the spatial memory dysfunction of mice with chronic cerebral hypoperfusion

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Journal of Sport and Health Science 2023年第1期12卷 116-129页

作者： Huawei Lin Jiayong Zhang Yaling Dai Huanhuan Liu Xiaojun He Lewen Chen Jing Tao Chaohui Li Weilin Liu College of Rehabilitation Medicine Fujian University of Traditional Chinese MedicineFuzhou 350122China Rehabilitation Industry Institute Fujian University of Traditional Chinese MedicineFuzhou 350122China General surgery Anxi General Hospital of Traditional Chinese MedicineQuanzhou 362400China

Background:Vascular cognitive impairment caused by chronic cerebral hypoperfusion(CCH)has become a hot issue *** exercise positively contributes to the preservation or restoration of cognitive abilities;however,the specific mechanism has remained *** recent studies found that neurogranin(Ng)is a potential biomarker for cognitive *** study aims to investigate the underlying role of Ng in swimming training to improve cognitive ***:To test this hypothesis,the clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9(Cas9)system was utilized to construct a strain of Ng conditional knockout(Ng cKO)mice,and bilateral common carotid artery stenosis(BCAS)surgery was performed to prepare the *** Experiment 1,2-month-old male and female transgenic mice were divided into a control group(wild-type littermate,n=9)and a Ng cKO group(n=9).Then,2-month-old male and female C57BL/6 mice were divided into a sham group(C57BL/6,n=12)and a BCAS group(n=12).In Experiment 2,2-month-old male and female mice were divided into a sham group(wild-type littermate,n=12),BCAS group(n=12),swim group(n=12),BCAS+Ng cKO group(n=12),and swim+Ng cKO group(n=12).Then,7 days after BCAS,mice were given swimming training for 5 weeks(1 week for adaptation and 4 weeks for training,5 days a week,60 min a day).After intervention,laser speckle was used to detect cerebral blood perfusion in the mice,and the T maze and Morris water maze were adopted to test their spatial ***,electrophysiology and Western blotting were conducted to record long-term potential and observe the expressions of Ca^(2+)pathway-related proteins,*** was applied to analyze the expression of relevant markers in neuronal damage,inflammation,and white matter ***:The figures showed that spatial memory impairment was detected in Ng cKO mice,and a sharp decline of cerebral blood flow and an impairment of p

关键词： Neurogranin swimming training Synaptic plasticity Vascular cognitive impairment

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Differences of Matrix Metalloproteinase 2 Expression between Left and Right Ventricles in Response to Nandrolone Decanoate and/or swimming training in Mice

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Chinese Medical Journal 2018年第2期131卷 207-212页

作者： Ying Bai Xu-Bo Shi Yu-Qiong Zhang Yue-Li Wang Xin-Yao Liu Maria Asuncion Esteve-Pastor Cardiovascular Center Beijing Tongren Hospital Capital Medical University Beijing 100730 China Department of Echocardiography Beijing Anzhen Hospital Capital Medical university National Clinical Research Center for Cardiovascular Diseases Beijing 100029 China Department of Cardiology Hospital Clinico Universitario Virgen'dela Arrixaca Instituto Murciano de Investigacion Biosanitaria (IMIB-Arrixaca) CIBER-CV Murcia 30120 Spain

Background： Matrix metalloproteinase （MMP）-2 plays an important role in the remodeling of left ventricles （LVs） and right ventricles （RVs）. We investigated the differences of MMP-2 expression between LV and RV in response to nandrolone dccanoate （ND）, swimming training （ST）, and combined ND and ST （NS） in mice, based on their structural, functional, and biochemical characteristics. Methods： Totally 28 male C57B1 mice （6 weeks old; 20-23 g） were divided into four groups, including the control （n = 7）, ND （n = 6）, ST （n = 8）, and NS （n = 7） groups. After respective treatments for 8 weeks, echocardiographic examination was used to assess the cardiac structure and function. Van Gieson stain was used to examine the fibrosis of LV and RV in response to different treatments, and Western blotting analysis was performed to explore different MMP-2 expressions between LV and RV in response to ND and/or ST. Analysis of variance was used for comparing the four groups. Results： At 8 weeks, right ventricular dimension/body weight in the ND group was larger than the other three groups （F = 7.12, P 〈 0.05） according to the echocardiographic examination. Fibrosis induced by ND administration was increased more in RV （2.59%） than that in LV （2.21%）. MMP-2 expression of the ND group in RV was significantly greater than the control and NS groups in RV and the corresponding ND group in LV. Conclusion： The experimental data support the hypothesis that ND administration induces greater MMP-2 expression increase in RV compared to LV, leading to consequent RV dilation.

关键词： Fibrosis Matrix Metalloproteinase 2 Nandrolone Decanoate swimming training

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