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Genetic identification and reiterated captures suggest that the Astyanax mexicanus El Pachón cavefish population is closed and declining

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Zoological Research 2023年第4期44卷 701-711页

作者： Laurent Legendre Julie Rode Isabelle Germon Marie Pavie Carla Quiviger Maxime Policarpo Julien Leclercq Stéphane Père Julien Fumey Carole Hyacinthe Patricia Ornelas-García Luis Espinasa Sylvie Rétaux Didier Casane UniversitéParis-Saclay CNRSIRDUMRÉvolutionGénomesComportement etÉcologieGif-sur-Yvette 91190France Objectif Sciences International NGO OSI-Panthera ProgramGeneva 99140Switzerland Institut de Neuroscience Paris-Saclay UniversitéParis-Saclay and CNRSSaclay 91400France Institut Pasteur UniversitéParis CitéCNRS UMR 2000Microbial Paleogenomics UnitParis 75015France Institut Pasteur UniversitéParis CitéBioinformatics and Biostatistics HubParis 75015France Department of Genetics Harvard Medical SchoolBlavatnik InstituteBoston MA 02115USA Colección Nacional de Peces Departamento de ZoologíaInstituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico DF CP 04510México School of Science Marist CollegePoughkeepsieNew York 12601USA UniversitéParis Cité UFR Sciences du VivantParis 75013France Zoological Institute Department of Environmental SciencesUniversity of BaselBaselSwitzerland

The sizes of Astyanax mexicanus blind cavefish populations of North-East Mexico are demographic parameters of great importance for investigating a variety of ecological,evolutionary,and conservation issues.However,few estimates have been obtained.For these mobile animals living in an environment difficult to explore as a whole,methods based on capture-mark-recapture are appropriate,but their feasibility and interpretation of results depend on several assumptions that must be carefully examined.Here,we provide evidence that minimally invasive genetic identification from captures at different time intervals(three days and three years)can give insights into cavefish population size dynamics as well as other important demographic parameters of interest.We also provide tools to calibrate sampling and genotyping efforts necessary to reach a given level of precision.Our results suggest that the El Pachón cave population is currently very small,of an order of magnitude of a few hundreds of individuals,and is distributed in a relatively isolated area.The probable decline in population size in the El Pachón cave since the last census in 1971 raises serious conservation issues.

关键词： Cavefish Population size Conservation swabbing Genetic identification

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Flexible surface-enhanced Raman scattering-active substrates based on nanofibrous membranes

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Nano Research 2018年第9期11卷 4468-4488页

作者： Ekaterina S. Prikhozhdenko Daniil N. Bratashov Dmitry A. Gorin Alexey M. Yashchenok Remote Controlled Theranostic Systems Lab Education and Research Institute of Nanostructures and Biosystems Saratov State University 83 As trakhanskaya Str. Saratov 410012 Russia Skoltech Center for Photonics and Quantum Materials Skolkovo Institute of Science and Technology Skolkovo Innovation Center 3 Nobel Str. Moscow 143026 Russia

Surface-enhanced Raman scattering （SERS） has emerged as an excellent analytical tool for the effective detection and fingerprint identification of various chemicals. Recently, significant progress has been made in the fabrication of SERS-active substrates using simple, inexpensive, and affordable methods. The full potential of universal SERS diagnostics will likely be realized with the development of approaches and devices capable of effectively detecting analytes on various surfaces as well as in multicomponent media. In addition, the combination of implantable or wearable SERS-active substrates and remote portable devices enables real-time diagnostics that ideally fit the concept of personalized medicine. In this paper, we summarize recent achievements in fabricating flexible SERS substrates made of cellulose paper, polymer membranes, and textile fibrous films. Emphasis is placed on the in-situ extraction and detection of various chemicals in real-world surfaces and complex media using flexible nanofibrous SERS platforms. The potential SERS applications and future perspectives in on-site diagnostics are also discussed.

关键词： paper electrospun membrane fabric surface-enhanced Ramanspectroscopy on-site diagnostics swabbing

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国外swabbing Equipment抽汲装置研究

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内蒙古石油化工 2012年第3期38卷 4-5页

作者：潘智勇中油辽河油田公司采油工艺处辽宁盘锦124010 东北石油大学黑龙江大庆163318

在试油作业中,为求目的层产量等参数时,若地层能量不足以使油井自喷,则采取抽汲等方式进行诱喷或求产。国外试油过程中抽汲作业多采用油管内抽汲,swabbing Equipment抽汲装置具有结构简单、安全系数和抽汲效率高的优点,值得国内从事试... 详细信息

在试油作业中,为求目的层产量等参数时,若地层能量不足以使油井自喷,则采取抽汲等方式进行诱喷或求产。国外试油过程中抽汲作业多采用油管内抽汲,swabbing Equipment抽汲装置具有结构简单、安全系数和抽汲效率高的优点,值得国内从事试油工作者借鉴和参考。

关键词：试油抽汲装置 swabbing Equipment 诱喷

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