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Long noncoding RNA HAND2-AS1 re duce d the viability of hepatocellular carcinoma via targeting microRNA-300/SOCS5 axis

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Hepatobiliary & Pancreatic Diseases International 2020年第6期19卷 567-574页

作者： Hua-Qiang Bi Zhong-Hui Li Hui Zhang Department of Hepatobiliary Surgery First Affiliated Hospital of Third Military Medical University(Army Medical University)Chongqing 400038China Department of Radiology First Affiliated Hospital of Third Military Medical University(Army Medical University)Chongqing 400038China Department of Vascular Surgery First Affiliated Hospital of Third Military Medical University(Army Medical University)Chongqing 400038China

Background:Hepatocellular carcinoma(HCC)is one of the most prevalent human cancers with high *** non-coding RNA heart and neural crest derivatives expressed 2 anti-sense 1(HAND2-AS1)is down-regulated in several cancers including HCC,yet the precise mechanisms how HAND2-AS1 regulates cell survival in HCC remains poorly ***:The expression levels of HAND2-AS1 and miR-300 were measured using quantitative real-time *** protein levels of suppressor of cytokine signaling 5(SOCS5),Bcl-2,Bax and cleaved caspase-3 were determined by Western *** viability and cell proliferation were assessed using cell counting kit-8 and clone formation assay,*** apoptosis was detected using flow *** interactions between HAND2-AS1 and miR-300,miR-300 and SOCS5 were validated using luciferase reporter ***:HAND2-AS1 was down-regulated in HCC tissues and cell lines,and the expression level of HAND2-AS1 was positively correlated to patient ***2-AS1 over-expression reduced viability and proliferation in HCC *** HAND2-AS1 level induced apoptosis in HCC cells,accompanied with increased Bax and cleaved caspase-3 levels and decreased Bcl-2 *** also validated that HAND2-AS1 acted as a sponge of miR-300,and there was a negative correlation between expression levels of HAND2-AS1 and miR-300 in HCC ***,we found that SOCS5 was a downstream target of *** addition,miR-300 mimics abolished HAND2-AS1-mediated inhibition of cell viability and ***-300 mimics also reversed the HAND2-AS1-induced apoptosis in HCC ***:lncRNA HAND2-AS1 inhibits proliferation in HCC through regulating miR-300/SOCS5 axis.

关键词： Hepatocellular carcinoma Cell survival Heart and neural crest derivatives expressed 2 anti-sense 1 microRNA-300 suppressor of cytokine signaling 5

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