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Blood meal analysis of tabanid fly after it biting the rare sumatran rhinoceros

Asian Pacific Journal of Tropical Biomedicine 2013年第2期3卷 95-99页

作者： Jeffrine Japning Rovie-Ryan Zainal Zahari Zainuddin Wahap Marni Abdul Hamid Ahmad Laurentius N.Ambu Junaidi Payne Ex-Situ Conservation Division Department of Wildlife and National Parks(DWNP)Peninsular MalaysiaMinistry of Natural Resources and Environment Malaysia(NRE) Department of Zoology Faculty of Resource Science and TechnologyUniversiti Malaysia Sarawak(UNIMAS) Borneo Rhino Alliance(BORA) Locked Bag 207388999 Kota KinabaluSabahMalaysia Institute for Tropical Biology and Conservation(ITBC) Universiti Malaysia Sabah(UMS) Sabah Wildlife Department

Objective:To demonstrate a noninvasive large mammalian genetic sampling method using blood meal obtained from a tabanid ***:Blood meal was recovered from the abdomen of an engorged tabanid fly(Haematopota sp.)which was captured immediately after biting a sumatran rhino in *** blood was applied on to a Whatman FTAblood *** laboratory work was conducted to extract,amplify and sequence the DNA from the *** was done by sampling the hair follicles and blood samples from the rhinoceros and subjecting it to the same laboratory ***:BLAST search and constructed phylogenetic trees confirmed the blood meal samples were indeed from the ***:This method could be used in the field application to noninvasively collect genetic *** of tabanids and other haematophagous arthropods(*** and ticks)and other blood-sucking parasites(*** and worms)could also provide information on vector-borne diseases.

关键词： Noninvasive DNA sampling Blood meal Tabanid fly sumatran rhino

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<i>Conyza sumatrensis</i>Intrapopulation Variation in Response to Glufosinate

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American Journal of Plant Sciences 2019年第9期10卷 1565-1582页

作者： Ivana Paula Ferraz Santos de Brito Caio Antonio Carbonari Bruna Barboza Marchesi Ana Karollyna Alves de Matos Leandro Tropaldi Nilda Roma-Burgos Edivaldo Domingues Velini Department of Crop Science Sao Paulo State University (UNESP) College of Agriculture Botucatu Sao Paulo Brazil Department of Crop Soil and Environmental Sciences University of Arkansas Fayetteville AR USA

We aimed to study the response of Conyza sumatrensis to different doses of glufosinate, intrapopulation variation in sensitivity to the herbicide, and the heritability of phenotypic response, and model the evolution of resistance. Three studies were conducted in the greenhouse with two repetitions. First, we tested doses of glufosinate (0, 50, 100, 200, 400, 800 g a.i. ha-1) plus a nontreated check, with four replications. Second, we examined the range in sensitivity of 44 plants to 200 g a.i. ha-1 glufosinate. Third, we evaluated the sensitivity of the progeny of six glufosinate-treated plants to 200 g a.i. ha-1 glufosinate. Plant response was evaluated visually and the ammonium content in leaf tissues was measured. Glufosinate at 400 g a.i. ha-1 caused the highest injury to *** plants. Ammonia accumulation occurred in response to glufosinate treatment, regardless of dose. Ammonia accumulation was correlated strongly with the level of visible plant injury;thus, it is a good indicator of herbicide efficacy. Sensitivity to glufosinate was highly variable within the population. Plants with high ammonia concentration (high injury) after treatment with glufosinate produced progenies that also had high ammonia concentrations after herbicide treatment. The variation in ammonia accumulation among siblings was high. Simulating the exclusion of plants that accumulated more ammonia produced a population that is expected to be less sensitive to glufosinate in the next generation. The stronger the selection pressures by a simulated treatment with glufosinate, the greater the reduction in ammonia accumulation in the progeny and expected sensitivity to glufosinate.

关键词： Ammonia Accumulation Differential Tolerance Glutamine Synthetase Herbicide Selection Pressure sumatran Fleabane

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