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Identification and expression analysis of sugar transporter family genes reveal the role of ZmSTP2 and ZmSTP20 in maize disease resistance

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Journal of Integrative Agriculture 2023年第11期22卷 3458-3473页

作者： MA Yu-xin ZHOU Zhi-jun CAO Hong-zhe ZHOU Fan SI He-long ZANG Jin-ping XING Ji-hong ZHANG Kang DONG Jin-gao State Key Laboratory of North China Crop Improvement and Regulation Hebei Agricultural UniversityBaoding 071000P.R.China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaoding 071000P.R.China Hebei Bioinformatic Utilization and Technological Innovation Center for Agricultural Microbes Hebei Agricultural UniversityBaoding 071000P.R.China Experimental Training Center of Hebei Agricultural University Hebei Agricultural UniversityBaoding 071000P.R.China

sugar is an indispensable source of energy for plant growth and development, and it requires the participation of sugar transporter proteins(STPs) for crossing the hydrophobic barrier in plants. Here, we systematically identified the genes encoding sugar transporters in the genome of maize(Zea mays L.), analyzed their expression patterns under different conditions, and determined their functions in disease resistance. The results showed that the mazie sugar transporter family contained 24 members, all of which were predicted to be distributed on the cell membrane and had a highly conserved transmembrane transport domain. The tissue-specific expression of the maize sugar transporter genes was analyzed, and the expression level of these genes was found to be significantly different in different tissues. The analysis of biotic and abiotic stress data showed that the expression levels of the sugar transporter genes changed significantly under different stress factors. The expression levels of Zm STP2 and Zm STP20 continued to increase following Fusarium graminearum infection. By performing disease resistance analysis of zmstp2 and zmstp20 mutants, we found that after inoculation with Cochliobolus carbonum, Setosphaeria turcica, Cochliobolus heterostrophus, and F. graminearum, the lesion area of the mutants was significantly higher than that of the wild-type B73 plant. In this study, the genes encoding sugar transporters in maize were systematically identified and analyzed at the whole genome level. The expression patterns of the sugar transporter-encoding genes in different tissues of maize and under biotic and abiotic stresses were revealed, which laid an important theoretical foundation for further elucidation of their functions.

关键词： maize sugar transporter gene expression disease resistance

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Genome-wide identification,expression and functional analysis of sugar transporters in sorghum(Sorghum bicolor L.)

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Journal of Integrative Agriculture 2022年第10期21卷 2848-2864页

作者： XIAO Qian-lin LI Zhen WANG Ya-yun HOU Xian-bin WEI Xi-mei ZHAO Xiao HUANG Lei GUO Yan-jun LIU Zhi-zhai College of Agronomy and Biotechnology Southwest UniversityChongqing 400715P.R.China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130P.R.China College of Agriculture and Food Engineering Baise UniversityBaise 533000P.R.China College of Animal Science and Technology Southwest UniversityChongqing 400715P.R.China

sugar transporters are essential for osmotic process regulation,various signaling pathways and plant growth and ***,few studies are available on the function of sugar transporters in sorghum(Sorghum bicolor L.).In this study,we performed a genome-wide survey of sugar transporters in *** total,98 sorghum sugar transporters(SSTs)were identified via *** SSTs were classified into three families based on the phylogenetic and conserved domain analysis,including six sucrose transporters(SUTs),23 sugars will eventually be exported transporters(SWEETs),and 69 monosaccharide transporters(MSTs).The sorghum MSTs were further divided into seven subfamilies,including 24 STPs,23 PLTs,two VGTs,four INTs,three p Glc T/SBG1 s,five TMTs,and eight *** localization of the SST genes showed that they were randomly distributed on 10 chromosomes,and substantial clustering was evident on the specific ***-seven SST genes from the families of SWEET,ERD,STP,and PLT were found to cluster in eight tandem repeat event *** total,22 SSTs comprising 11 paralogous pairs and accounting for 22.4%of all the genes were located on the duplicated *** different subfamilies of SST proteins possessed the same conserved domain,but there were some differences in features of the motif and transmembrane helices(TMH).The publicly-accessible RNA-sequencing data and real-time PCR revealed that the SST genes exhibited distinctive tissue specific *** studies showed that seven SSTs were mainly located on the cell membrane and membrane organelles,and 14 of the SSTs could transport different types of monosaccharides in *** findings will help us to further elucidate their roles in the sorghum sugar transport and sugar signaling pathways.

关键词： sorghum(Sorghum bicolor L.) sugar transporter SUT SWEET MST phylogenetic analysis

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Impaired SWEET-mediated sugar transportation impacts starch metabolism in developing rice seeds

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The Crop Journal 2022年第1期10卷 98-108页

作者： Pei Li Lihan Wang Hongbo Liu Meng Yuan National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070HubeiChina

sugar transportation and sugar-to-starch metabolism are considered important processes in seed development and embryo viability.A few plant SWEET proteins acting as sugar transporters have been reported to function in inflorescence and/or seed ***,we identified seven members of the 21 Os SWEET genes in rice that play essential roles in sugar transportation and sugar-to-starch conversion in seed *** Os SWEET genes exhibiting different expression patterns during inflorescence and seed development were knocked out individually by CRISPR/*** third of the mutants showed decreased fertile pollen viability and shriveled mature caryopses,resulting in weakened seed *** fill-related genes but not representative grain shape-regulating genes showed attenuated expression in the *** of each of these mutants accumulated more sucrose,glucose or fructose but less *** all Os SWEET genes,Os SWEET4 and Os SWEET11 had major effects on caryopsis *** sugar-to-starch metabolic pathway was significantly altered in ossweet11 mutants based on differential expression analysis in RNA sequencing assays,confirming that Os SWEET11 functions as a sugar transporter with a key role in seed *** results help to decipher the multiple functions of Os SWEET genes and to show how they might be used in genetic improvement of rice.

关键词： Oryza sativa sugar transporter SWEET sugar Starch

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Functional characterization of a novel, highly expressed ion-driven sugar antiporter in the thoracic muscles of Helicoverpa armigera

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Insect Science 2022年第1期29卷 78-90页

作者： Yi-Yang Yuan Yu-Cui Xin Jian-Li Han Yu-Han Zhao Shi-Ming Han Zi-Yan Nangong Xin Chen Zhu-Cheng Wang Mei Li Xing-Hui Qiu State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina College of Life Sciences University of Chinese Academy of SciencesBeijingChina College of Life Sciences Cangzhou Normal UniversityCangzhouJiangsu ProvinceChina College of Plant Protection Hebei Agricultural UniversityBaodingHebei ProvinceChina

sugar transporters(STs),which mainly mediate cellular sugar exchanges,play critical physiological roles in living organisms,and they may be responsible for sugar exchanges among various insect ***,the molecular and physiological functions of insect STs are largely ***,16 STs of Helicoverpa armigera were identified.A phylogenetic analysis classified the putative HaSTs into 12 sub-families,and those identified in this study were distributed into 6 ***-time polymerase chain reaction indicated that the 16 HaSTs had diverse tissue-specific expression *** transporter,HaST10,was highly expressed in thoracic muscles.A functional study using a Xenopus oocyte expression system revealed that HaST10 mediated both H+-driven trehalose and Na+-driven glucose antiport activities with high transport efficiency and low affinity levels.A HaST10 knockout clearly impaired the performance of ***,HaST10 may participate in sugar-supply regulation and have essential physiological roles in ***.

关键词： CRISPR/Cas9 glucose Helicoverpa armigera sugar transporter trehalose thoracic muscles

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Genome-wide identification, characterization, and expression analysis of the SWEET gene family in cucumber

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Journal of Integrative Agriculture 2017年第7期16卷 1486-1501页

作者： HU Li-ping ZHANG Feng SONG Shu-hui TANG Xiao-wei XU Hui LIU Guang-min WANG Ya-qin HE Hong-ju Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) Ministry of Agriculture

SWEETs （sugars will eventually be exported transporters） are a novel class of recently identified sugar transporters that play important roles in diverse physiological processes. However, only a few species of the plant SWEETgene family have been functionally identified. Up till now, there has been no systematic analysis of the SWEETgene family in Cucurbitaceae crops. Here, a genome-wide characterization of this family was conducted in cucumber（Cucumis sativus L.）. A total of 17 CsSWEETgenes were identified, which are not evenly distributed over the seven cucumber chromosomes. Cucumber SWEET protein sequences possess seven conserved domains and two putative serine phosphorylation sites. The phylo- genetic tree of the SWEET genes in cucumber, Arabidopsis thaliana, and Oryza sativa was constructed, and all the SWEET genes were divided into four clades. In addition, a number of putative cis-elements were identified in the promoter regions of these CsSWEET genes： nine types involved in phytohormone responses and eight types involved in stress responses. Moreover, the transcript levels of CsSWEETgenes were analyzed in various tissues using quantitative real-time polymerase chain reaction. A majority （70.58%） of the CsSWEET genes were confined to reproductive tissue development. Finally, 18 putative watermelon ClaSWEETgenes and 18 melon CmSWEETgenes were identified that showed a high degree of similarity with CsSWEETgenes. The results from this study provided a basic understanding of the CsSWEETgenes and may also facilitate future research to elucidate the function of SWEET genes in cucumber and other Cucurbitaceae crops.

关键词： cucumber gene expression phylogenetic analysis sugar transporter SWEET watermelon

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Genome-wide identification and transcriptome profiling reveal great expansion of SWEET gene family and their wide-spread responses to abiotic stress in wheat (Triticum aestivum L.)

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Journal of Integrative Agriculture 2020年第7期19卷 1704-1720页

作者： QIN Jin-xia JIANG Yu-jie LU Yun-ze ZHAO Peng WU Bing-jin LI Hong-xia WANG Yu XU Sheng-bao SUN Qi-xin LIU Zhen-shan State Key Laboratory of Crop Stress Biology for Arid Areas College of AgronomyNorthwest A&F UniversityYangling 712100P.R.China Department of Plant Genetics&Breeding China Agricultural UniversityBeijing 100193P.R.China College of Landscape and Ecological Engineering Hebei University of EngineeringHandan 056021P.R.China

The sugars Will Eventually be Exported Jransporter(SWEET)gene family,identified as sugar transporters,has been demonstrated to play key roles in phloem loading,grain filling,pollen nutrition,and plant-pathogen *** date,the study of SWEET genes in response to abiotic stress is very *** this study,we performed a genome-wide identification of the SWEET gene family in wheat and examined their expression profiles under mutiple abiotic *** identified a total of 105 wheat SWEET genes,and phylogenic analysis revealed that they fall into five clades,with clade V specific to wheat and its closely related *** the 105 wheat SWEET genes,59%exhibited significant expression changes after stress treatments,including drought,heat,heat combined with drought,and salt stresses,and more up-regulated genes were found in response to drought and salt *** hierarchical clustering analysis revealed that SWEET genes exhibited differential expression patterns in response to different stress treatments or in different wheat ***,different phylogenetic clades also showed distinct response to abiotic stress ***,we found that homoeologous SWEET genes from different wheat subgenomes exhibited differential expression patterns in response to different abiotic stress *** genome-wide analysis revealed the great expansion of SWEET gene family in wheat and their wide participation in abiotic stress *** expression partitioning of SWEET homoeologs under abiotic stress conditions may confer greater flexibility for hexaploid wheat to adapt to ever changing environments.

关键词： wheat sugar transporter abiotic stress homoeologous gene expression partitioning

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Deciphering the genetic basis of silkworm cocoon colors provides new insights into biological coloration and henotypic diversification

Deciphering the genetic basis of silkworm cocoon colors prov...

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中国蚕学会2023年学术年会

作者： Yaru Lu Jiangwen Luo Erxia An Bo Lu Yinqiu Wei Xiang Chen Kunpeng Lu Shubo Liang Hai Hu Minjin Han Songzhen He Jianghong Shen Dongyang Guo Nvping Bu Ling Yang Wenya Xu Cheng Lu Zhonghuai Xiang Xiaoling Tong Fangyin Dai State Key Laboratory of Resource Insects Southwest University

The genetic basis of phenotypic variation is a long-standing concern of evolutionary *** has proven to be a visual,easily quantifiable,and highly tractable system for genetic analysis and is an ever-evolving focus of biological *** with the homogenized brownyellow cocoons of wild silkworms,the cocoons of domestic silkworms are spectacularly diverse in color,such as white,green,and yellow-red;this provides an outstanding model for exploring the phenotypic diversification and biological ***,the molecular mechanism underlying silkworm green cocoon formation was investigated,which was not fully *** demonstrated that five of the seven members of a sugar transporter gene cluster were specifically duplicated in the Bombycidae and evolved new spatial expression patterns predominantly expressed in silk glands,accompanying complementary temporal expression;they synergistically facilitate the uptake of flavonoids,thus determining the green ***,polymorphic cocoon coloring landscape involving multiple loci and the evolution of cocoon color from wild to domestic silkworms were analyzed based on the pan-genome sequencing *** was found that cocoon coloration involved epistatic interaction between loci;all the identified cocoon color-related loci existed in wild silkworms;the genetic segregation,recombination,and variation of these loci shaped the multi-colored cocoons of domestic *** study revealed a new mechanism for flavonoids-based biological coloration that highlights the crucial role of gene duplication followed by functional diversification in acquiring new genetic functions;furthermore,the results in this work provide insight into phenotypic innovation during domestication.

关键词： Phenotypic variation Domestication Coloration Flavonoids sugar transporter

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