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Molecular characterization,expression patterns,and subcellular localization of a classical and a novel nonclassical MHC class Iαmolecules from Japanese eel Anguilla japonica

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Aquaculture and Fisheries 2023年第2期8卷 108-123页

作者： Jianjun Feng Xinwei Peng Peng Lin Yilei Wang Ziping Zhang Yuankai Xu Pengyun Chen Jingying Wu Engineer Research Center of Eel Modern Industry Technology Ministry of EducationXiamen361021China College of Fisheries Jimei UniversityXiamen361021China College of Animal Science Fujian Agriculture and Forestry UniversityFuzhou350002China Ningbo Institute of Oceanography Ningbo315832China

Major histocompatibility complex(MHC)molecules play a critical role in the immune response of vertebrate animals by presenting foreign antigens to T *** this study,we first cloned and identified a classical and a novel nonclassical MHC Iαmolecules from Japanese eel(Anguilla japonica),named as AjMHC I-UBA and AjMHC I-L,*** full-length cDNA of AjMHC I-UBA contains an open reading frame(ORF)of 1047 bp encoding a predicted protein of 348 amino acids,while AjMHC I-L 1089 bp encodes 362 amino *** multiple alignment of the amino acid sequence showed that AjMHC I-UBA and AjMHC I-L consist of an N-terminal MHC I superfamily domain withinα1 andα2 helices,an IgC-MHC Iα3 domain,and a transmembrane region close to the C-terminal,which are similar to other fish and mammal *** polymorphism analysis showed that eight different major alleles of AjMHC I-UBA,named as AjMHC I-UBA*0101~1001,were identified from six Japanese eel ***,a distinguishing signature of the nonclassical L-lineage genes specific motif“HINMTL”,including an N-glycosylation site(NXS/T),was present in theα3 domain of AjMHC I-L *** the predicted three-dimensional structures of AjMHC I-UBA and AjMHC I-L are similar to that of human MHC Iα,phylogenetic analysis showed that these two protein molecules belong to classical MHC I UBA gene of U-lineage and nonclassical MHC I gene of L-lineage,*** real-time polymerase chain reaction(qRT-PCR)analysis revealed that the highest expression of AjMHC I-UBA and AjMHC I-L was found in the intestine and the expression level of AjMHC I-UBA in all of the examined tissues was significantly higher than that of AjMHC *** expressions of AjMHC I-UBA and AjMHC I-L in liver,kidney and spleen were significantly induced following injection with the viral mimic poly I:C,LPS,and Aeromonas hydrophila *** vitro,the AjMHC I-UBA and AjMHC I-L transcripts of Japanese eel liver cells were si

关键词： Anguilla japonica MHC Iα mRNA expression subcelluar localization

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NB-ARC RGAs enhance resistibility to stripe rust in Triticum aestivum but not the resistance gene from genetics

NB-ARC RGAs enhance resistibility to stripe rust in Triticum...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： ZHANG Hong WANG Chang-You WANG Ya-Juan JI Wan-Quan State Key Laboratory of Crop Stress Biology for Arid Areas College of Agronomy(Northwest A&F University)

Wheat(Triticum aestivum) production is seriously threatened by Puccinia striiformis f. *** West(Pst) worldwide,and R-genes are considered the most cost-effective stratagem to minimize the *** most abundant R genes belong to the NB-LRR *** date,hundreds of R genes with NB-LRR domains have been reported and dissected in arabidopsis,rice,barley,maize, and so on,whereas knowledge concerning NB-ARC genes has been limited,especially in hexaploid *** wheat NB-ARC orthologues were identified and characterized,and named TaYRGs(YRGIa,1b,2-6).The cDNA of TaYRG1a consisted of 4147 nucleotides and contained an open reading frame of 2889 nucleotides encoding a polypeptide of 962 amino acids(aa),while TaYRG6 encoded a polypeptide of 136 *** estimated molecular weight of TaYRG1 protein was 108.2 kD with a pI of *** with TaYRG1,the constructs of TaYRG2,YRG3,YRG4, YRG5 and YRG6,showed 675+63,1537,2107,2387 and 2478-bp deletions but contain conserved 5’and 3’ ***,the 2387-bp deletion was replaced with a 551-bp insertion in TaYRG5 Since all of these genes are captured from mRNA and appeared in order following the primary TaYRGi,we inferred that TaYRG2,YRG3,YRG4 and YRG6 were transcribed with exon skipping and alternative 5’ and 3’ splicing site types in the process of post-transcription,while TaYRG5 was formed with intron *** exclusive exons exited in the proceeding of TaYRG4 and *** to TMpred,four transmembrane helices existed at *** analysis indicated that TaYRGs were involved in plant defense against Pst ***,in the compatible interaction,transcript accumulation was nearly *** levels in hyper-susceptible lines,Xiaoyan 22 and Mingxian 169 are substantially lower than that in the common immune wheat lines Shaamnai 139 and Yuanfeng ***,the expression of TaYRGs in immune hexaploid wheat was significantly greater than that in diploid species at

关键词： wheat Puccinia striiformis f.sp.tritici West NB-ARC subcelluar localization RNAi,enhanced immune resistance

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