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Expression of stromal cell-derived factor-1 in diabetic retinopathy

Chinese Medical Journal 2010年第8期123卷 984-988页

作者： CHEN Ling-yan ZHUO Ye-hong LI Yong-hao HUANG Xin-hua ZHANG Jing-lin LI Shi-yi WANG Xiang-gui Lu Lin Center Sun Yat-sen University Guangzhou Guangdong 510060 China

Background Neovascularization can cause vision loss in proliferative diabetic retinopathy （PDR） and may be affected by many factors. stromal cell-derived factor-1 （SDF-1） is a potent stimulator of angiogenesis. The study was aimed to investigate the expression of SDF-1 and its correlation with vascular endothelial growth factor （VEGF） in the eyes with diabetic retinopathy. Methods The levels of SDF-1 and VEGF were measured by enzyme-linked immunosorbent assay in the vitreous of 41 eyes of 41 patients with PDR and 12 eyes of 12 patients with idiopathic macular hole （IMH）. Vitreous fluid samples and fibrovascular preretinal membranes were obtained at vitrectomy. SDF-1 and VEGF were localized using immunohistochemistry. Results The vitreous concentration of VEGF was significantly higher in eyes with PDR （（2143.7~1685.21） pg/ml） than in eyes with IMH （（142.42~72.83） pg/ml, P 〈0.001）. The vitreous level of SDF-1 was also significantly higher in eyes with PDR （（306.37~134.25） pg/ml） than in eyes with IMH （（86.91~55.05） pg/ml, P 〈0.001）. The concentrations of both VEGF and SDF-1 were higher in eyes with active PDR than in eyes with inactive PDR. Panretinal photocoagulation （PRP） could decrease the SDF-1 levels in the vitreous of PDR patients. The vitreous concentration of SDF-1 correlated with that of VEGF in eyes with PDR （r=0.61, P 〈0.001）. The costaining of SDF-1 and VEGF was confined to the vascular components in preretinal membranes. Conclusions SDF-1 protein is highly expressed in both the vitreous and preretinal membranes of PDR patients; SDF-1 may be correlated with VEGF in angiogenesis in PDR.

关键词： diabetic retinopathy stromal cell-derived factor vascular endothelial growth factor

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Tropism mechanism of stem cells targeting injured brain tissues by stromal cell-derived factor-1

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Chinese Journal of Traumatology 2009年第5期12卷 -页

作者： ZHANG Sai LIU Xiao-zhi LIU Zhen-lin SHANG Chong-zhi HU Qun-liang Department of Neurosurgery Affiliated Hospital of Medical College of Chinese People's Armed Police ForceTianjin 300162 China

Objective: To explore the role and function of stromal cell-derived factor- 1 (SDF- 1) in stem cells migrating into injured brain ***: Rat-derived nerve stem cells (NSCs) were isolated and cultured routinely. Transwell system was used to observe the migration ability of NSCs into injured nerve cells. Immunocytochemistry was used to explore the expression of chemotactic factor receptor-4 (CXCR-4) in NSCs. In vivo, we applied immunofluorescence technique to observe the migration of NSCs into injured brain area. Immunofluorescence technique and Western blotting were used to test expression level of SDF- 1. After AMD3100 (a special chemical blocker) blocking CXCR-4, the migration ability of NSCs was tested in vivo and in vitro, ***: NSCs displayed specific tropism for injured nerve cells or traumatic brain area in vivo and in vitro. The expression level of SDF-1 in traumatic brain area increased remarkably and the expression level of CXCR-4 in the NSCs increased simultaneously. After AMD3100 blocking the expression of CXCR-4, the migration ability of NSCs decreased significantly both in vivo and in ***: SDF-1 may play a key role in stem cells migrating into injured brain area through specially combining with CXCR-4.

关键词： stromal cell-derived factor Stem cells Chemotactic factor Traumatic brain injury

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