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AtDREB2A-CA Influences Root Architecture and Increases Drought Tolerance in Transgenic Cotton

Agricultural Sciences 2017年第10期8卷 1195-1225页

作者： Maria Eugenia Lisei-de-Sa Fabricio B.M.Arraes Giovani G.Brito Magda A.Beneventi Isabela T.Lourenco-Tessutti Angelina M.M.Basso Regina M.S.Amorim Maria C.M.Silva Muhammad Faheem Nelson G.Oliveira Junya Mizoi Kazuko Yamaguchi-Shinozaki Maria Fatima Grossi-de-Sa Embrapa Recursos Genéticos e Biotecnologia BrasíliaBrazil Empresa de Pesquisa Agropecuária de Minas Gerais UberabaBrazil Universidade Federal do Rio Grande do Sul Porto AlegreBrazil Embrapa Clima Temperado PelotasBrazil Universidade de Brasília BrasíliaBrazil Embrapa Agroenergia BrasíliaBrazil Japan International Research Center for Agricultural Sciences(JIRCAS) TsukubaJapan Universidade Catolica de Brasilia BrasiliaBrazil

Drought is a major environmental factor limiting cotton (Gossypium hirsutum L.) productivity worldwide and projected climate changes could increase their negative effects in the future. Thus, targeting the molecular mechanisms correlated with drought tolerance without reducing productivity is a challenge for plant breeding. In this way, we evaluated the effects of water deficit progress on AtDREB2A-CA transgenic cotton plant responses, driven by the stress-inducible rd29 promoter. Besides shoot and root morphometric traits, gas exchange and osmotic adjustment analyses were also included. Here, we present how altered root traits shown by transgenic plants impacted on physiological acclimation responses when submitted to severe water stress. The integration of AtDREB2A-CA into the cotton genome increased total root volume, surface area and total root length, without negatively affecting shoot morphometric growth parameters and nor phenotypic evaluated traits. Additionally, when compared to wild-type plants, transgenic plants (17-T0 plants and its progeny) highlighted a gradual pattern of phenotypic plasticity tosome photosynthetic parameters such as photosynthetic rate and stomatal conductance with water deficit progress. Transgene also promoted greater shoot development and root robustness (greater and deeper root mass) allowing roots to grow into deeper soil layers. The same morpho-physiological trend was observed in the subsequent generation (17.6-T2). Our results suggest that the altered root traits shown by transgenic plants are the major contributors to higher tolerance response, allowing the AtDRE2A-CA-cotton plants to maintain elevated stomatal conductance and assimilate rates and, consequently, reducing their metabolic costs involved in the antioxidant responses activation. These results also suggest that these morpho-physiological changes increased the number of reproductive structures retained per plant (26% higher) when compared with its non-transgenic cou

关键词： Dehydration Responsive Element Binding Factors Water Deficit Tolerance Gossypium hirsutum Physiological Phenotyping Transcription Factor stress-inducible promoter

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The characteristics in phenotype and drought tolerance of the transgenic tobacco plants by induced overexpression of TaEXPB23 gene from wheat

The characteristics in phenotype and drought tolerance of th...

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从植物科学到农业发展——2012全国植物生物学大会

作者： Feng Li,Yangyang Han,Yanan Feng,Shichao Xing,Meirong Zhao,Jin Zhang, Wei Wang~* State Key Laboratory of Crop Biology,Shandong Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tai’an,Shandong,271018.

Expansins are the key regulators of cell wall extension during plant *** our previous study,the drought tolerance of transgenic tobacco plants was improved by overexpressing a wheat expansin gene TaEXPB23 driven by a constitutive 35S cauliflower mosaic virus (CaMV) promoter,but the development and phenotype of transgenic plants were severely retarded under normal *** this paper,we found that the cellular characteristics and carbohydrate metabolism were changed in 35S::TaEXPB23 transgenic tobacco plants. Then,the suitability of expressing TaEXPB23 driven by a stress-inducible RD29A promoter in order to improve the phenotype of the transgenic tobacco plants was *** determination of physiological and biochemical parameters indicated that RD29A::TaEXPB23 transgenic tobacco plants exhibited enhanced tolerance in comparison with wild-type *** study demonstrates that the use of stress-inducible promoter, such as RD29A,may minimize the unfavorable phenotype caused by constitutive expression of transgene and improve the drought tolerance.

关键词： Drought tolerance Expansin stress-inducible promoter Transgenic plant

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