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Arbuscular mycorrhizal fungi inoculation and exogenous indole-3-acetic acid application induce antioxidant defense response to alleviate cadmium toxicity in Broussonetia papyrifera

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Pedosphere 2024年第2期34卷 447-459页

作者： Xue LI Jingwei LIANG Hongjian WEI Yuxuan KUANG Hui CHEN Ming TANG Wentao HU State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern AgricultureGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant GermplasmCollege of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642China

Cadmium(Cd)contamination in soil poses a huge threat to plants even at low concentrations;Broussonetia papyrifera has great potential in remediation of soil heavy metal contamination.However,whether exogenous indole-3-acetic acid(IAA)application and arbuscular mycorrhizal fungi(AMF)have synergistic effects on Cd tolerance of B.papyrifera remains unclear.To investigate the effects of AMF inoculation and IAA application on the tolerance of B.papyrifera to Cd stress,two experiments were conducted:the first to investigate the effect of AMF(Rhizophagus irregularis)inoculation on the tolerance of B.papyrifera to Cd stress and the second to investigate the combined effects of AMF inoculation and IAA application on the tolerance of B.papyrifera to Cd stress.Parameters including endogenous hormone concentration,antioxidant defense response,malondialdehyde(MDA)content,and gene expression related to antioxidant enzyme system and hormone were measured.The results indicated that AMF alleviated Cd toxicity of B.papyrifera by reducing MDA content and improving antioxidant enzyme activities and Cd absorption capacity.Furthermore,the combination of AMF inoculation and IAA application had a synergetic effect on the tolerance of B.papyrifera to Cd stress through upregulating BpAUX1 and BpAUX2,which might contribute to root growth and root xylem synthesis,and by upregulating BpSOD2 and BpPOD34 to enhance the antioxidant enzyme system.This work provides a new insight into the application of IAA in the remediation of soil Cd pollution by mycorrhizal plants.

关键词： enzyme activity geneexpression heavy metal phytoremediation remediation Rhizophagus irregularis soil cadmium pollution stress tolerance

Increased Vitamin C Content Accompanied by an Enhanced Recycling Pathway Confers Oxidative stress tolerance in Arabidopsis

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Journal of Integrative Plant Biology 2010年第4期52卷 400-409页

作者： zinan wang Ying Xiao Wansheng Chen Kexuan Tang Lei Zhang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University Shanghai 200433 China Department of Pharmacognosy School of Pharmacy Second Military Medical University Shanghai 200433 China Department of Pharmacy Changzheng Hospital second Military Medical University Shanghai 200003 China

Vitamin C （L-ascorbic acid, AsA） has important antioxidant and metabolic functions in both plants and animals. Once used, ascorbic acid can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase （DHAR, EC 1.***.5.1）. To analyze the physiological role of DHAR catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, we examined whether increasing the level of AsA through enhanced AsA recycling would limit the deleterious effects of oxidative stress. A chimeric construct consisting of the double CaMV35S promoter fused to the Myc-dhar gene was introduced into Arabidopsis thaliana. Transgenic plants were biochemically characterized and tested for responses to oxidative stress. Western blot indicated that the dhar-transgene was successfully expressed. In homozygous T4 transgenic seedlings, DHAR overexpression was increased up to 1.5 to 5.4 fold, which enhanced foliar ascorbic acid levels 2- to 4.25-fold and ratio of AsA/DHA about 3- to 16-fold relative to wild type. In addition, the level of glutathione, the reductant used by DHAR, also increased as did its redox state. When whole plants were treated with high light and high temperature stress or in vitro leaf discs were subjected to 10μM paraquat, transgenic plants showed a larger AsA pool size, lower membrane damage, and a higher level of chlorophyll compared with controls. These data suggested that increasing the plant vitamin C content through enhanced ascorbate recycling could limit the deleterious effects of environmental oxidative stress.

关键词： stress tolerance Increased Vitamin Enhanced Recycling

Reduction of methylviologen-mediated oxidative stress tolerance in antisense transgenic tobacco seedlings through restricted expression of StAPX

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 578-585页

作者： Wei-hong SUN Yong WANG Hua-gang HE Xue LI Wan SONG Bin DU Qing-wei MENG College of Food and Biological Engineering Jiangsu University State Key Laboratory of Crop Biology College of Life Science Shandong Agricultural University

Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic to- bacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress.

关键词： Methylviologen Oxidative stress StAPX stress tolerance Transgenic tobacco seedling

IiWRKY34 positively regulates yield, lignan biosynthesis and stress tolerance in Isatis indigotica

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Acta Pharmaceutica Sinica B 2020年第12期10卷 2417-2432页

作者： Ying Xiao Jingxian Feng Qing Li Yangyun Zhou Qitao Bu Junhui Zhou Hexin Tan Yingbo Yang Lei Zhang Wansheng Chen Research and Development Center of Chinese Medicine Resources and Biotechnology Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Changzheng HospitalNaval Medical University(Second Military Medical University)Shanghai 200003China Department of Pharmaceutical Botany School of PharmacyNaval Medical University(Second Military Medical University)Shanghai 200433China National Resource Center for Chinese Meteria Medica China Academy of Chinese Medical SciencesBeijing 100700China Kanion Research Institute Shanghai University of Traditional Chinese MedicineShanghai 201203China

Yield potential,pharmaceutical compounds production and stress tolerance capacity are 3 classes of traits that determine the quality of medicinal plants.The autotetraploid Isatis indigotica has greater yield,higher bioactive lignan accumulation and enhanced stress tolerance compared with its diploid progenitor.Here we show that the transcription factor IiWRKY34,with higher expression levels in tetraploid than in diploid I.indigotica,has large pleiotropic effects on an array of traits,including biomass growth rates,lignan biosynthesis,as well as salt and drought stress tolerance.Integrated analysis of transcriptome and metabolome profiling demonstrated that IiWRKY34 expression had far-reaching consequences on both primary and secondary metabolism,reprograming carbon flux towards phenylpropanoids,such as lignans and flavonoids.Transcript-metabolite correlation analysis was applied to construct the regulatory network of IiWRKY34 for lignan biosynthesis.One candidate target Ii4CL3,a key rate-limiting enzyme of lignan biosynthesis as indicated in our previous study,has been demonstrated to indeed be activated by IiWRKY34.Collectively,the results indicate that the differentially expressed IiWRKY34 has contributed significantly to the polyploidy vigor of I.indigotica,and manipulation of this gene will facilitate comprehensive improvements of I.indigotica herb.

关键词： Polyploidy vigor WRKY transcription factor Biomass production Lignan biosynthesis stress tolerance

Phytoremediation,stress tolerance and bio fortification in crops through soilless culture

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Crop Design 2023年第1期2卷 13-28页

作者： Avinash Sharma Himanshu Pandey V.S.Devadas Bhagya D.Kartha Amit Vashishth Faculty of Agricultural Sciences Arunachal University of StudiesNamsaiArunachal Pradesh792103India Division of Plant Physiology and Biochemistry ICAR-Indian Institute of Sugarcane ResearchLucknowUttar Pradesh226005India Vanavarayar Institute of Agriculture PollachiTamil Nadu642103India Department of Fruit Crops College of AgricultureKerala Agricultural UniversityThrissurKerala680656India Department of Science and Humanities SRM Institute of Science and TechnologyGhaziabadUttar Pradesh201204India

Crop improvement through soilless cultivation opens up challenges of crop breeding,plant tissue culture and gene manipulation.The plant growing in soilless culture in a closed system,vertical farming,and protected cultivation under controlled climatic factors has immense potential for crop improvement in different aspects of phytoremediation,stress tolerance and bio fortification.The water-based culture and substrate-based culture induce classified stress tolerance in crops such as disease stress,water stress,salt stress under controlled climatic factors and can also be utilized to develop bio fortified crops.The scope and possibilities of crop improvement through water-based culture and substrate-based culture with respect to phytoremediation,stress tolerance,bio fortifi-cation,mechanisms of crop improvement and the challenges in crop improvement through soilless culture are reviewed in this paper.The soilless culture has enormous tools to combat problem of crop improvement and call for soilless green revolution.The comparative and non-comparative investigations of metabolomics,phenomics,genomics and proteomics,Donald concept-plant ideotype and Mendel theory-oligogenic and polygenic inheritance are essential to examine in natural&artificial system through soilless crop improvement.

关键词： Water culture Substrate stress tolerance Nutrient Crop improvement Gene manipulation

Nanotechnology:A promising field in enhancing abiotic stress tolerance in plants

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Crop Design 2023年第2期2卷 1-18页

作者： Mawuli K.Azameti Abdul-Wahab M.Imoro Department of Applied Biology C.K.Tedam University of Technology and Applied SciencesNavrongoUpper East RegionGhana

Heavy metals,drought,salinity,cold stress,and heat stress are some of the main abiotic stresses that adversely affect plant growth and crop productivity generally.For crop production systems to be sustainable in the face of abiotic environmental constraints,effective strategies must be used in conjunction with technological advancement to achieve this goal.In recent times,the emergence of nanotechnology as an intriguing field of study with application potential in the field of agriculture needs to be given the necessary attention to address some of the abiotic stresses.Due to their environmental friendliness,affordability,special physicochemical properties,and increased plant productivity,the use of nanoparticles(NPs)as nano fertilizers has enormous potential and much interest has developed in the field in recent times.Abiotic stress management involves NPs,according to several studies.In order to create a practical and environmentally responsible plan for the long-term sustainability of agriculture,this review focuses on the effects of abiotic stress on plants,synthesis,and the roles of NPs in managing abiotic stresses,and future prospects.

关键词： Nanotechnology Abiotic stress Nanoparticles stress tolerance

Research Progress of miR164 and Target Gene NAC in Regulating Sugarcane stress tolerance

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Plant Diseases and Pests 2023年第4期14卷 44-47页

作者： Minghui CHEN Junqing WANG Xuyao ZHAO Shiping CHENG Henan Provincial Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Wood Plant Pingdingshan UniversityPingdingshang 467000China Guangxi Key Laboratory of Sugarcane Genetic Improvement Nanning 530000China

Studies have shown that miR164 is a highly conserved miRNA family between monocot and dicotyledonous plants,and it plays an important role in the growth and development of sugarcane organs and in response to stress.As the main target gene of miR164,NAC transcription factors are mainly regulated at the post-transcriptional level.MiR164:NAC module may play an important role in determining the adaptive response of sugarcane to stress.MiR164 has a regulatory effect on the expression of target gene NAC,and may be closely related to the resistance process of sugarcane to abiotic stress,which provides a reference for using miRNA to carry out sugarcane resistance molecular breeding.

关键词： miRNA164 NAC stress tolerance

Mapping of QTLs for Leaf Malondialdehyde Content Associated with stress tolerance in Rice

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Rice science 2009年第1期16卷 72-74页

作者： JIANG Jing ZHUANG Jie-yun FAN Ye-yang SHEN Bo College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310012 China Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China

Malondialdehyde （MDA） is the final product of lipid peroxidation, and MDA content can reflect the stress tolerance of plants. To map QTLs conditioning the MDA content in rice leaves, a recombinant inbred line （RIL） population with 247 lines derived from an indica-indica cross Zhenshan 97BxMilyang 46, and a linkage map consisting of 207 DNA markers were used. The RIL population showed a transgressive segregation in the MDA content of rice leaves. Two QTLs for the MDA content in rice leaves were detected in the intervals RG532-RG811 and RG381-RG236 on chromosome 1, with the additive effects from maternal and paternal parents, accounting for 4.33% and 4.62% of phenotype variations, respectively.

关键词： rice malondialdehyde content quantitative trait locus leaf stress tolerance

Overexpression of AmDUF1517 enhanced tolerance to salinity, drought, and cold stress in transgenic cotton

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Journal of Integrative Agriculture 2018年第10期17卷 2204-2214页

作者： HAO Yu-qiong LU Guo-qing WANG Li-hua WANG Chun-ling GUO Hui-ming LI Yi-fei CHENG Hong-mei Biotechnology Research Institute Chinese Academy of Agricultural Sciences

As abiotic stresses become more severe as a result of global climate changes, the growth and development of plants are restricted. In the development of agricultural crops with greater stress tolerance, AmDUF1517 had been isolated from the highly stress-tolerant shrub Ammopiptanthus mongolicus, and can significantly enhance stress tolerance when inserted in Arabidopsis thaliana. In this study, we inserted this gene into cotton to analyze its potential for conferring stress tolerance. Two independent transgenic cotton lines were used. Southern blot analyses indicated that AmDUF1517 was integrated into the cotton genome. Physiological analysis demonstrated that AmD UF1517-transgenic cotton had stronger resistance than the control when treated with salt, drought, and cold stresses. Further analysis showed that trans-AmD UF1517 cotton displayed significantly higher antioxidant enzyme（superoxide dismutase（SOD）, peroxidase（POD）, catalase（CAT）, and glutathione S-transferase（GST）） activity and less reactive oxygen species（ROS） accumulation, which suggests that overexpression of AmDUF1517 can improve cotton resistance to stress by maintaining ROS homeostasis, as well as by alleviating cell membrane injury. These results imply that AmDUF1517 is a candidate gene in improving cotton resistance to abiotic stress.

关键词： transgenic cotton stress tolerance AmDUF1517 Ammopiptanthus mongolicus reactive oxygen species