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Lipid phosphorylation by a diacylglycerol kinase suppresses ABA biosynthesis to regulate plant stress responses

Molecular Plant 2024年第2期17卷 342-358页

作者： Jianwu Li Shuaibing Yao Sang-Chul Kim Xuemin Wang Department of Biology University of Missouri-St.LouisSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA

Lipid phosphorylation by diacylglycerol kinase(DGK)that produces phosphatidic acid(PA)plays important roles in various biological processes,including stress responses,but the underlying mechanisms remain elusive.Here,we show that DGK5 and its lipid product PA suppress ABA biosynthesis by interacting withABA-DEFICIENT2(ABA2),a key ABA biosynthesis enzyme,to negatively modulate plant responseto abiotic stress tested in Arabidopsis thaliana.Loss of DGK5 function rendered plants less damaged,whereas overexpression(OE)of DGK5 enhanced plant damage to water and salt stress.The dgk5 mutant plants exhibited decreased total cellular and nuclear levels of PA with increased levels of diacylglycerol,whereas DGK5-OE plants displayed the opposite effect.Interestingly,we found that both DGK5 and PA bind to the ABA-synthesizing enzyme ABA2 and suppress its enzymatic activity.Consistently,the dgk5 mutant plants exhibited increased levels of ABA,while DGK5-OE plants showed reduced ABA levels.In addition,we showed that both DGK5 and ABA2 are detected in and outside the nuclei,and loss of DGK5 function decreased the nuclear association of ABA2.We found that both DGK5 activity and PA promote nuclear association of ABA2.Taken together,these results indicate that both DGK5 and PA interact with ABA2 to inhibit its enzymatic activity and promote its nuclear sequestration,thereby sup-pressing ABA production in response to abiotic stress.Our study reveals a sophisticated mechanism by which DGK5 and PA regulate plant stress responses.

关键词： diacylglycerol kinase phosphatidic acid diacylglycerol lipid signaling stress responses lipid-protein binding protein-protein interaction Arabidopsis

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Influences of dexmedetomidine on stress responses and postoperative cognitive and coagulation functions in patients undergoing radical gastrectomy under general anesthesia

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World Journal of Gastrointestinal Surgery 2023年第6期15卷 1169-1177页

作者： Xiang-Fei Ma Shi-Jia Lv Shen-Qiao Wei Bing-Rong Mao Xiu-Xia Zhao Xiao-Qing Jiang Fei Zeng Xue-Ke Du Department of Anesthesiology The Second Affiliated Hospital of Guangxi Medical UniversityNanning 530007Guangxi Zhuang Autonomous RegionChina

BACKGROUND Radical gastrectomy(RG)is commonly used in the treatment of patients with gastric cancer(GC),but this procedure may lead to stress responses,postoperative cognitive dysfunction,and blood coagulation abnormalities in patients.AIM To investigate the influences of dexmedetomidine(DEX)on stress responses and postoperative cognitive and coagulation functions in patients undergoing RG under general anesthesia(GA).METHODS One hundred and two patients undergoing RG for GC under GA from February 2020 to February 2022 were retrospectively reviewed.Of these,50 patients had received conventional anesthesia intervention[control group(CG)]and 52 patients had received DEX in addition to routine anesthesia intervention[observation group(OG)].Inflammatory factor(IFs;tumor necrosis factor-α,TNF-α;interleukin-6,IL-6),stress responses(cortisol,Cor;adrenocorticotropic hormone,ACTH),cognitive function(CF;Mini-Mental State Examination,MMSE),neurological function(neuron-specific enolase,NSE;S100 calciumbinding protein B,S100B),and coagulation function(prothrombin time,PT;thromboxane B2,TXB2;fibrinogen,FIB)were compared between the two groups before surgery(T0),as well as at 6 h(T1)and 24 h(T2)after surgery.RESULTS Compared with T0,TNF-α,IL-6,Cor,ACTH,NSE,S100B,PT,TXB2,and FIB showed a significant increase in both groups at T1 and T2,but with even lower levels in OG vs CG.Both groups showed a significant reduction in the MMSE score at T1 and T2 compared with T0,but the MMSE score was notably higher in OG compared with CG.CONCLUSION In addition to a potent inhibitory effect on postoperative IFs and stress responses in GC patients undergoing RG under GA,DEX may also alleviate the coagulation dysfunction and improve the postoperative CF of these patients.

关键词： Dexmedetomidine Radical gastrectomy General anesthesia Inflammatory factors stress responses

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The transcription factor FgNsf1 regulates fungal development,virulence and stress responses in Fusarium graminearum

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Journal of Integrative Agriculture 2021年第8期20卷 2156-2169页

作者： SHI Dong-ya REN Wei-chao WANG Jin ZHANG Jie Jane Ifunanya MBADIANYA MAO Xue-wei CHEN Chang-jun College of Plant Protection Nanjing Agricultural UniversityNanjing 210095P.R.China College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109P.R.China

Nutrient and stress factor 1(Nsf1),a transcription factor containing the classical Cys2-His2(C_(2)H_(2))zinc finger motif,is expressed under non-fermentable carbon conditions and in response to salt stress in Saccharomyces cerevisiae.However,the role of Nsf1 in filamentous fungi is not well understood.In this study,the orthologue of Nsf1 was investigated in Fusarium graminearum(named FgNsf1),a causal agent of Fusarium head blight(FHB).The functions of FgNsf1 were evaluated by constructing a FgNSF1 deletion mutant,designated asΔFgNsf1,and its functional complementation mutantΔFgNsf1-C.Gene deletion experiments showed that the mycelial growth rate,asexual and sexual reproduction ofΔFgNsf1 were significantly reduced,but the pigment production ofΔFgNsf1 was remarkably increased compared with the PH-1 andΔFgNsf1-C.In addition,the tolerance ofΔFgNsf1 to osmotic pressures,cell wall-damaging agents and oxidative stress increased significantly.Sensitivity tests to different fungicides revealed thatΔFgNsf1 exhibited increased sensitivity to carbendazim(MBC)and tebuconazole,and enhanced tolerance to fludioxonil and iprodione than PH-1 andΔFgNsf1-C.The virulence ofΔFgNsf1 to wheat coleoptiles and flowering wheat heads were dramatically decreased,which was consistent with the decrease in the yield of deoxynivalenol(DON).All of these defects were restored by target gene complementation.These results indicated that FgNsf1 plays a crucial role in vegetative growth,asexual and sexual reproduction,stress responses,fungicide sensitivity,and full virulence in F.graminearum.

关键词： Fusarium graminearum nutrient and stress factor 1 stress responses virulence

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Loss of behavioral stress response in blind cavefish reduces energy expenditure

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Zoological Research 2023年第4期44卷 678-692页

作者： Jiang-Hui Zhang Rui Long Yang-Yang Jing Pan Zhang Yuan Xu Wei Xiong Yan-Qiu Zhu Yi-Ping Luo Key Laboratory of Freshwater Fish Reproduction and Development Ministry of EducationSchool of Life SciencesSouthwest UniversityChongqing 400715China

The stress response is essential for animal self-defense and survival. However, species may exhibit stress response variation depending on their specific environmental and selection pressures. Blind cavefish dwell in cave environments, which differ markedly in stressors and resource availability compared to surface aquatic environments. However, whether blind cavefish exhibit differences in stress response as an adaptation to their cave environments remains unclear. Here, we investigated differences in stress response in six closely related Triplophysa species, including three blind cavefish (T. longibarbata, T. jiarongensis, and T. rosa) and three normal-sighted river fish (T. nasobarbatula, T. dongsaiensis, and T. bleekeri). Results showed that blind cavefish exhibited a range of distinct behavioral responses compared to sighted river fish, including greater levels of activity, shorter duration of freezing, absence of erratic movements or thrashing behavior, and opposite behavioral trends over time. Furthermore, the cavefish species demonstrated attenuated increases in metabolic rate in response to stressors related to novel environments. Cave-dwelling T. rosa also exhibited lower basal hypothalamic-pituitary-inter-renal (HPI) axis-related gene expression levels and stress hormone concentrations compared to river-dwelling T. bleekeri. These results suggest that blind cavefish may have lost their behavioral stress response, potentially mediated by a reduction in basal activity of the HPI axis, thus enabling the conservation of energy by reducing unnecessary expenditure in energy-limited caves.

关键词： Cavefish stress responses Behavior Metabolic rate

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Nitric oxide negatively regulates gibberellin signaling to coordinate growth and salt tolerance in Arabidopsis

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Journal of Genetics and Genomics 2022年第8期49卷 756-765页

作者： Lichao Chen Shuhao Sun Chun-Peng Song Jian-Min Zhou Jiayang Li Jianru Zuo State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesBeijing 100101China State Key Laboratory of Crop stress Adaptation and Improvement Collaborative Innovation Center of Crop Stress BiologyHenan UniversityKaifengHenan 475001China Hainan Yazhou Bay Seed Laboratory SanyaHainan 572025China

In response to dynamically altered environments,plants must finely coordinate the balance between growth and stress responses for their survival.However,the underpinning regulatory mechanisms remain largely elusive.The phytohormone gibberellin promotes growth via a derepression mechanism by proteasomal degradation of the DELLA transcription repressors.Conversely,the stress-induced burst of nitric oxide(NO)enhances stress tolerance,largely relying on NO-mediated S-nitrosylation,a redox-based posttranslational modification.Here,we show that S-nitrosylation of Cys-374 in the Arabidopsis RGA protein,a key member of DELLAs,inhibits its interaction with the F-box protein SLY1,thereby preventing its proteasomal degradation under salinity condition.The accumulation of RGA consequently retards growth but enhances salt tolerance.We propose that NO negatively regulates gibberellin signaling via S-nitrosylation of RGA to coordinate the balance of growth and stress responses when challenged by adverse environments.

关键词： Gibberellin Nitric oxide S-nitrosylation DELLA repressors stress responses

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Genomic polymorphisms at the crhr2 locus improve feed conversion efficiency through alleviation of hypothalamus-pituitary-interrenal axis activity in gibel carp(Carassius gibelio)

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Science China(Life Sciences) 2022年第1期65卷 206-214页

作者： Chuang Shi Qiyong Lou Beide Fu Junyan Jin Jianfei Huang Yao Lu Xia Jin Jiangyan He Gang Zhai Shouqi Xie Jingou Tong Jianfang Gui Zhan Yin State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100864China

Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish supplies.Here,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),which is involved in hypothalamuspituitary-interrenal(HPI)axis signaling,is associated with improved FCE in farmed allogynogenetic gibel carp strain CAS Ⅲ compared with that in the wild gibel carp strain Dongting(DT).This set of polymorphisms downregulates the expression levels of crhr2 mRNA in the brain and pituitary tissues in gibel carp strain CAS Ⅲ compared with those in strain DT.Furthermore,compromised HPI axis signaling is observed in gibel carp strain CAS Ⅲ,such as decreased α-melanocyte stimulating hormone protein levels,plasma cortisol content,and stress responses.Moreover,enhanced activation of protein kinase B/mammalian target of rapamycin complex 1 signaling observed in the muscle tissue of strain CAS Ⅲ in comparison to that in strain DT indicated elevated anabolic metabolism in strain CAS Ⅲ.Thus,these studies demonstrate that the genetic markers associated with compromised HPI axis signaling,such as crhr2,are potentially useful for genetic selection toward improvement in farmed fish growth and FCE,which would reduce fishmeal consumption and thereby indirectly facilitate sustainable fisheries.

关键词： feed conversion efficiency crhr2 stress responses endocrine regulation genetic marker for fish breeding

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Advances in proteome-wide analysis of plant lysine acetylation

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Plant Communications 2022年第1期3卷 115-134页

作者： Linchao Xia Xiangge Kong Haifeng Song Qingquan Han Sheng Zhang Key Laboratory of Bio-Resource and Eco-Environment of the Ministry of Education College of Life SciencesSichuan UniversityChengdu 610065China

Lysine acetylation(LysAc)is a conserved and important post-translational modification(PTM)that plays a key role in plant physiological and metabolic processes.Based on advances in Lys-acetylated protein immunoenrichment and mass-spectrometric technology,LysAc proteomics studies have been performed in many species.Such studies have made substantial contributions to our understanding of plant LysAc,revealing that Lys-acetylated histones and nonhistones are involved in a broad spectrum of plant cellular processes.Here,we present an extensive overview of recent research on plant Lys-acetylproteomes.We provide in-depth insights into the characteristics of plant LysAc modifications and the mechanisms by which LysAc participates in cellular processes and regulates metabolism and physiology during plant growth and development.First,we summarize the characteristics of LysAc,including the properties of Lys-acetylated sites,the motifs that flank Lys-acetylated lysines,and the dynamic alterations in LysAc among different tissues and developmental stages.We also outline a map of Lys-acetylated proteins in the Calvin–Benson cycle and central carbon metabolism–related pathways.We then introduce some examples of the regulation of plant growth,development,and biotic and abiotic stress responses by LysAc.We discuss the interaction between LysAc and Na-terminal acetylation and the crosstalk between LysAc and other PTMs,including phosphorylation and succinylation.Finally,we propose recommendations for future studies in the field.We conclude that LysAc of proteins plays an important role in the regulation of the plant life cycle.

关键词： lysine acetylproteomes modified characteristics plant growth and development stress responses PTM crosstalk

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Transcriptome-based Discovery of Fusarium graminearum stress responses to FgHV1 Infection

Transcriptome-based Discovery of Fusarium graminearum Stress...

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中国植物病理学会2016年学术年会

作者： WANG Shuang-chao ZHANG Jing-ze LI Peng-fei QIU De-wen GUO Li-hua State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Science Walloon Centre of Industrial Biology Gembloux Agro-Bio TechUniversity of Liege

Fusarium graminearum hypovirus 1(FgHV1),which is phylogenetically related to Cryphonectria hypovirus 1(CHV1),is a virus in the family Hypoviridae that infects the plant pathogenic fungus F.graminearum.Although hypovirus FgHV1 infection results in defects in mycelial growth and spore production,it does not attenuate the virulence of the host(hypovirulence).We now report that the vertical transmission rate of FgHV1 through asexual spores reached 100%and that hypovirus FgHV1 infection did not alter phenotypes such as pigment production and sexual development Using RNA deep sequencing,we performed genome-wide expression analysis to reveal pheno type-related genes with expression changes in response to FgHV1 infection.A total of 248 geneswere differentially expressed,suggesting that hypovirus infection causes a significant alteration of fungal gene expression.Nearly two times as many genes were up-regulated as were down-regulated.A differentially expressed gene enrichment analysis identified a number of important pathways.Metabolic processes,cellular redox regulation,the ubiquitination system and transcription factors were the most affected categories in F.graminearum challenged with FgHV1.Papain-like protease,p20,encoded by FgHV1 could cause HO accumulation in Nicotiana benthamiana leaves and programmed cell death in N.tabacum cv.Samsun NN leaves.Moreover,hypovirus FgHV1 may trigger the RNA silencing pathway in F.graminearum.

关键词： Fusarium graminearum FgHV1 Transcriptome stress responses Cellular redox regulation

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An Argonaute gene of Valsa mali Plays Distinct Roles in the stress responses and Pathogenicity

An Argonaute gene of Valsa mali Plays Distinct Roles in the ...

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中国植物病理学会2016年学术年会

作者： FENG Hao XU Ming LIU Yang-yang GAO Xiao-ning YIN Zhi-yuan HUANG Li-li College of Plant Protection and State Key Laboratory of Crop stress Biology for Arid Areas Northwest A&F University

Valsa mali(V.mali),the causative agent of apple tree Valsa canker,causes heavy damage to apple production.Exploration of the pathogenesis of V.mali will contribute to the generation of effective disease control strategies.The Argonaute proteins(AGOs),as the core components of the RNA-induced silencing complex(RISC),play key roles in RNA interference,which is important for various cellular processes.In this study,one AGO gene of V.mali was found to be dramatically up-regulated during infection of the pathogen.Functions of this gene in growth,stresses response and pathogenicity of V.mali was analyzed by constructing gene knock-out mutant.Compared to the wild-type strain03-8(WT),the colonial morphology and growth rate of mutant showed no significant difference on PDA plates,but the hypha morphology of mutant showed increased branches.Moreover,no obvious altered colonial morphology was observed for mutant under treatment of NaCl(0.1mol/L,0.5mol/L and1.0mol/L),KC1(0.5mol/L,1.0mol/L and 1.5mol/L) and different pH value(2.01.0).However,application of 0.05%HO led to the growth stop of mutant.In addition,the mutant exhibited decreased pathogenicity.Our results revealed the important roles of an AGO gene in stress tolerance and pathogenicity of V.mali,which may lay foundation for exploring the mechanisms of RNA interference of V.mali.

关键词： Argonaute protein Pathogenicity Small RNA stress responses Apple Valsa canker

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Aquaporinl Regulates development,Secondary Metabolism and stress responses in Fusarium graminearum

Aquaporinl Regulates development,Secondary Metabolism and St...

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中国植物病理学会2017年学术年会

作者： Ding Mingyu Li Jing Fan Xinyue Yu Xiaoyang He Fang Xu Houjuan Liang Yuancun Yu Jinfeng College of Plant Protection Shandong Agricultural University

The Ascomycete fungus Fusarium graminearum is the causative agent of Fusarium head blight,and has become the predominant model organism for study of fungal phytopathogens.Aquaporins（AQPs） have been implicated in the transport of water,glycerol,and a variety of other small molecules in plants and animals.However,the role of these proteins is poorly understood in plant pathogenic fungi.Here,we identified and attempted to elucidate the function of five aquaporin genes in the F.graminearum genome.A phylogenetic analysis of aquaporins revealed that AQPs are divided into two clades,AQP1 belongs to the first clade.Deletion of AQP\ is responsible for the regulation of development,stress response,sexual and asexual reproduction.The AAQPl mutant was fully pathogenic similar as the wild type,whilethe mutant was increased in DON production.In addition,we attempt to characterise the cellular localisation of AQP1 using GFP fusions,and we found that AQP1 appears to be located to the nuclear membranes and vacuole membranes in conidia.Notably,except to localize to the nuclear membranes and vacuole membranes,we also found that AQP1 is localized to the endoplasmic reticulum inmycelial growth stage.Taken together,these results suggest that AQP1 is involved in development,stress response,sexual and asexual reproduction.

关键词： Aquaporin Fusarium graminearum Development Secondary metabolism stress responses

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