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A potent fish pathogenic bacterial killer streptomyces *** from the soils of east coast region,South India

Journal of Coastal Life Medicine 2013年第3期 175-180页

作者： Durairaj Thirumurugan Ramasamy Vijayakumar Department of Microbiology Bharathidasan University Constituent College Kurumbalur-621107 PerambalurTamilnaduIndia

Objective:To investigate the potentiality of the marine actinobacteria isolated from marine soil against fish pathogenic ***:east coast region(ecr)of Tamilnadu,South *** they were used for the isolation of actinobacteria by using conventional serial dilution technique on starch casein agar *** antibacterial activities of the actinobacteria were screened primarily by using cross streak plate method against fish pathogenic bacteria namely Vibrio alginolyticus,Vibrio parahaemolyticus,Vibrio cholera,Aeromonas *** Pseudomonas *** antimicrobial efficacy of the selected isolates was carried out with various organic solvents,and finally the active compound was subjected to chromatographic techniques including TLC and ***:In the present study,a total of 33 soil samples were collected from the Bay of Bengal,against fish pathogenic *** of 21 antibacterial isolates,the isolate ecr77 was selected for further study based on its potential activity against fish pathogenic *** the various solvents tested,the ethyl acetate extract had good antibacterial activity against the tested bacterial *** isolate ecr77 grew well on oat meal agar medium with 2%salt level at 35°***-MS study found that the presence of bioactive compounds namely tetradecanoic acid,n-hexadecanoic acid and octadecanoic *** morphological,physiological,biochemical and cultural characteristics of the potential isolate were supported the identity up to generic level asstreptomyces ***:The results obtained from this study concludes that the ecr soils of South India is a hot spot of novel bioactive compound producing marine actinobacteria with great pharmaceutical *** the 82 actinobacteria isolated,21(26%)isolates were possessed antibacterial activity.

关键词： East coast region South India streptomyces sp.ecr77 Antibacterial activity GC-MS Decanoic acids

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