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Journal of Materials Science & Technology 2021年第7期66卷 112-120页

作者： Hanyu Zhao Yupeng Sun Lu Yin Zhao Yuan Yiliang Lan Dake Xu Chunguang Yang Ke Yang School of Materials Science and Engineering University of Science and Technology of ChinaShenyang110016China Institute of Metal Research Chinese Academy of SciencesShenyang110016China Shenyang National Laboratory for Materials Science Northeastern UniversityShenyang110819China

304 stainless steel(SS) used as orthodontic wire during orthodontics faces the risk of microbiologically influenced corrosion(MIC) due to diverse flora environment. Hereinto, streptococcus mutans(S. mutans)is the most important cariogenic bacteria. In this work, MIC behavior of a new 304-Cu SS in presence of S. mutans was studied by the observations using scanning electron microscopy(SEM) and confocal laser scanning microscopy(CLSM) including live/dead staining, extracellular polymeric substance(EPS)staining and pitting corrosion, electrochemical test, and X-ray photoelectron spectroscopy(XPS). Above results showed that 304-Cu SS possessed excellent biofilm inhibition ability and presented lower corrosion current density(icorr), larger polarization resistance(Rp) and charge transfer resistance(Rct) in the presence of S. mutans, indicating that 304-Cu SS had a better MIC resistance against S. mutans. It was further affirmed by XPS results that the presence of Cu-oxide in passive film of 304-Cu SS inhibited the formation of biofilm.

关键词： 304-Cu stainless steel Microbiologically influenced corrosion streptococcus mutans Biofilm

Deactivation of streptococcus mutans Biofilms on a Tooth Surface Using He Dielectric Barrier Discharge at Atmospheric Pressure

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Plasma Science and Technology 2013年第6期15卷 535-541页

作者： Imola MOLNAR Judit PAPP Alpar SIMON Sorin Dan ANGHEL Babes-Bolyai University Faculty of Biology and GeologyCluj-Napoca 400015Romania Babes-Bolyai University Faculty of PhysicsCluj-Napoca 400084Romania

This paper presents a study of the effect of the low temperature atmospheric helium dielectric barrier discharge（DBD） on the streptococcus mutans biofilms formed on tooth surface. Pig jaws were also treated by plasma to detect if there is any harmful effect on the *** plasma was characterized by using optical emission *** data indicated that the discharge is very effective in deactivating streptococcus mutans *** can destroy them with an average decimal reduction time（D-time） of 19 s and about 98%of thein were killed after a treatment time of 30 *** to the survival curve kinetic an overall 32 s treatment time would be necessary to perform a complete *** experimental results presented in this study indicated that the helium dielectric barrier discharge,in plan-parallel electrode configuration,could be a very effective tool for deactivation of oral bacteria and might be a promising technique in various dental clinical applications.

关键词： streptococcus mutans dielectric barrier discharge plasma treatment biofilm deactivation sterilization

The Antibacterial Activities of Copper Oxide Nanoparticles Synthesized Using Laser Ablation in Different Surfactants against streptococcus mutans

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Journal of Renewable Materials 2023年第5期11卷 2109-2123页

作者： Ruaa H.Abbas A.Kadhim Azhar M.Haleem Laser and Optoelectronics Engineering Department University of Technology-IraqBaghdadIraq Environmental Research Center University of Technology-IraqBaghdadIraq

Copper oxide nanoparticles(CuO NPs)were synthesised with laser ablation of a copper sheet immersed in deionized water(DW),cetrimonium bromide(CTAB),and sodium dodecyl sulphate(SDS),*** target was irradiated with a pulsed Nd:YAG laser at 1064 nm,600 mJ,a pulse duration of 10 ns,and a repetition rate of 5 *** CuO NPs colloidal were analyzed using UV–Vis spectroscopy,the Fourier transform infrared(FTIR)spectrometer,zeta potential(ZP),X-ray diffraction(XRD),transmission electron microscope(TEM)and field emission scanning electron microscopy(FESEM).The absorption spectra of CuO NPs colloidal showed peaks at 214,215 and 220 nm and low-intensity peaks at 645,650 and 680 nm for SDS,CTAB and DW,*** NPs’colloidal results are(−21.6,1.2,and 80 mV)for negatively,neutrally,and positively charged SDS,DW,and CTAB,*** XRD pattern of the NPs revealed the presence of CuO phase planes(110)(111),(20-2)and(11-1).The TEM images revealed nearly spherical NPs,with sizes ranging from 10–90,10–50,and 10–210 nm for CuO NPs mixed with DW,SDS and CTAB,*** images of all the synthesized samples illustrate the formation of spherical nanostructure and large particles are *** CuO NPs were tested for antibacterial activity against streptococcus mutans by using the well diffusion *** this method,CuO NPs prepared in DW at a concentration of 200μg/mL showed a greater inhibition zone against streptococcus mutans.

关键词： Copper oxide nanoparticles(CuO NPs) laser ablation streptococcus mutans

Differential Proteomics Analysis on Fluoride-resistant streptococcus mutans by Label-free Quantitation

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Chemical Research in Chinese Universities 2014年第4期30卷 566-571页

作者： ZHAO Hongyan ZHANG Zhimin ZHU Laikuan LI Zhenling LI Penglian LIU Jiabai ZHANG Zhiying School of Stomatology Jilin University Changchun 130021 P. R. China Shanghai Key Laboratory of Stomatology The Ninth People's Hospital School of Medicine Shanghai Jiaotong University Shanghai 200011 P. R. China

To figure out the changed biological characteristics of a fluoride-resistant strain compared with those of the parent sensitive strain of streptococcus mutans（S,mutans） with the help of differential proteomics analysis,sixtyseven proteins with obvious differential expression were identified in fluoride-resistant *** them are the important virulence factors causing caries,including glucosyltransferase-S,glucosyltransferase-I,glucosyltransferase-Si,major cell-surface adhesin Pac,F-ATPase β-subunit,Fth,molecular chaperone DnaK,chaperonin GroEL,and the lactate *** obtained the differential proteomics expression profiles of the fluoride-resistant strain（UA159-FR） and its parental strain（UA159） of *** important virulence factors bringing about caries were identified to have differential expressions,among which the expression of lactate dehydrogenase in fluoride-resistant strain was enhanced compared to that in its parent *** results could provide the basis for analvsing the functions of proteins encoded by caries-related genes from the fluoride-resistant *** strain.

关键词： Fluoride-resistant streptococcus mutans Differential proteomics Label-free quantitation

The synergistic effect of honey and cinnamon against streptococcus mutans bacteria

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Asian Pacific Journal of Tropical Biomedicine 2017年第4期7卷 314-320页

作者： Mohammad Bagher Rezvani Mohammad Niakan Mohammad Kamalinejad Fateme Sadat Ahmadi Faeze Hamze Operative Department Shahed Dental SchoolShahed University Microbiology Department Shahed Medical SchoolShahed University School of Pharmacy Shahid Beheshti University of Medical Sciences Dental Research Center Shahed Dental SchoolShahed University Oral and Dental Disease Research Center Kerman University of Medical Science

Objective: To investigate the effect of Iranian honey, cinnamon and their combination against streptococcus mutans ***: Nine experimental solutions were examined in this study, including two types of honey(pasteurized and sterilized), two types of cinnamon extract(dissolved in distilled water or dimethyl sulfoxide) and five different mixtures of cinnamon in honey(prepared by admixing 1%–5% w/w of cinnamon extract into 99%–95% w/w of honey, respectively).Meanwhile, each of mentioned agent was considered as the first solution while it was diluted into seven serially two-fold dilutions(from 1:2 to 1:128 v/v).Therefore, eight different concentrations of each agent were *** antibacterial tests were performed through blood agar well diffusion method, and the minimum inhibitory concentration(MIC) was ***, the data were subjected to statistical analysis incorporating Two-way ANOVA and Bonferroni post hoc tests(a = 0.01).Results: The highest zone of inhibition was recorded for the mixtures of honey and cinnamon while all the subgroups containing 95%–99% v/v of honey were in the same range(P streptococcus mutans while there was no significant difference among extracts containing 99%–95% v/v of honey admixing with 1%–5% v/v of cinnamon, respectively.

关键词： Synergistic effect Honey Cinnamon streptococcus mutans

Construction and in vitro Expression of streptococcus mutans Surface Protein Encoding DNA Vaccine

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第4期21卷 269-272页

作者：彭志翔樊明文边专陈智彭斌 Stomatological Center of Tongji Hospital Huazhong University of Science and Technology Wuhan 430030

DNA vaccine plasmids were constructed that encoded two highly conservative regions of a surface protein, PAc, from the human major cariogenic bacterium, streptococcus mutans . Antigen expression was evaluated in vitro by immunohistochemical analysis of human endothelial cells following cationic liposome mediated transient transfection with recombinant plasmid. The results of this study provided a basis for further testing of these recombinant plasmids in primates and for efficacy testing of dental caries DNA vaccines in human volunteers in future.

关键词： streptococcus mutans surface protein DNA vaccine

Antibacterial activity of nanostructured Ti-45S5 bioglass-Ag composite against streptococcus mutans and Staphylococcus aureus

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Transactions of Nonferrous Metals Society of China 2016年第1期26卷 118-125页

作者： K.JURCZYK M.M.KUBICKA M.RATAJCZAK M.U.JURCZYK K.NIESPODZIANA D.M.NOWAK M.GAJECKA M.JURCZYK Department of Conservative Dentistry and Periodontology Poznan University of Medical Sciences Faculty of Pharmacy Department of Genetics and Pharmaceutical MicrobiologyPoznan University of Medical Sciences Division of Mother's and Child's Health Poznan University of Medical Sciences Institute of Materials Science and Engineering Poznan University of Technology Institute of Human Genetics Polish Academy of Sciences

Mechanical alloying and annealing at 1150 °C for 2 h under an argon atmosphere were used to prepare Ti-45S5 bioglass nanocomposites. Ti-45S5 bioglass material was chemically modified by silver. The antibacterial activity of Ti-10% 45S5 bioglass nanocomposite containing silver against streptococcus mutans and Staphylococcus aureus was studied. Nanocomposites were characterized by X-ray diffraction, scanning electron microscopy equipped with an electron energy dispersive spectrometer and transmission electron microscopy to evaluate phase composition, crystal structure and grain size. In vitro bacterial adhesion study indicated a significantly reduced number of streptococcus mutans and Staphylococcus aureus on the bulk nanostructured Ti-45S5 bioglass-Ag plate surface in comparison to that on microcrystalline Ti plate surface. Nanostructured Ti-based biomaterials can be considered to be the future generation of dental implants.

关键词： 45S5 bioglass antimicrobial activity silver titanium dental implant streptococcus mutans Staphylococcus aureus

The photodynamic therapy on streptococcus mutans biofilms using erythrosine and dental halogen curing unit

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International Journal of Oral Science 2012年第4期4卷 196-201页

作者： Young-Ho Lee Ho-Won Park Ju-Hyun Lee Hyun-Woo Seo Si-Young Lee Department of Pediatric Dentistry Graduate SchoolCollege of DentistryGangneung-Wonju National University Department of Pediatric Dentistry College of DentistryGangneung-Wonju National University Department of Microbiology and Immunology College of DentistryGangneung-Wonju National University

The purpose of our study was to evaluate the effect of photodynamic therapy （PDT）, using erythrosine as a photosensitizing agent and a dental halogen curing unit as a light source, on streptococcus mutans in a biofilm phase. The S. mutans biofilms were formed in a 24-well cell culture cluster. Test groups consisted of biofilms divided into four groups： group 1： no photosensitizer or light irradiation treatment （control group）; group 2： photosensitizer treatment alone; group 3： light irradiation alone; group 4： photosensitizer treatment and light irradiation. After treatments, the numbers of colony-forming unit （CFU） were counted and samples were examined by confocal laser scanning fluorescence microscopy （CLSM）. Only group 4 （combined treatment） resulted in significant increases in cell death, with rates of 75% and 55% after 8 h of incubation, and 74% and 42% at 12 h, for biofilms formed in brain-heart infusion （BHI） broth supplemented with 0% or O. 1% sucrose, respectively. Therefore, PDT of S. mutans biofilms using a combination of erythrosine and a dental halogen curing unit, both widely used in dental clinics, resulted in a significant increase in cell death. The PDT effects are decreased in biofilms that form in the presence of sucrose.

关键词： biofilms dental halogen curing unit erythrosine photodynamic therapy streptococcus mutans

Targeted Antimicrobial Therapy Against streptococcus mutans Establishes Protective Non-cariogenic Oral Biofilms and Reduces Subsequent Infection

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International Journal of Oral Science 2010年第2期2卷 66-73页

作者： Li-na Li Li-hong Guo Renate Lux Randal Eckert Daniel Yarbrough Jian He Maxwell Anderson Wen-yuan Shi UCLA School of Dentistry University of Califomia Los Angeles USA UCLA Molecular Biology Institute University of Califomia Los Angeles USA Department of Oral Biology Peking University Dental School Beijing China C3 Jian Inc Inglewood USA

Aim Dental biofilms are complex communities composed largely of harmless bacteria. Certain pathogenic species including streptococcus mutans （S. mutans） can become predominant when host factors such as dietary sucrose intake imbalance the biofilm ecology. Current approaches to control S. mutans infection are not pathogen-specific and eliminate the entire oral community along with any protective benefits provided. Here, we tested the hypothesis that removal of S. mutans from the oral community through targeted antimicrobial therapy achieves protection against subsequent S. mutans colonization. Methodology Controlled amounts of S. mutans were mixed with S. mutans-free saliva, grown into biofilms and visualized by antibody staining and cfu quantization. Two specifically-targeted antimicrobial peptides （STAMPs） against S. mutans were tested for their ability to reduce S. mutans biofilm incorporation upon treatment of the inocula. The resulting biofilms were also evaluated for their ability to resist subsequent exogenous S. mutans colonization. Results S. mutans colonization was considerably reduced （9 ± 0.4 fold reduction, P=0.01） when the surface was preoccupied with saliva-derived biofilms. Furthermore, treatment with S. mutans-specific STAMPs yielded S. mutans-deficient biofilms with significant protection against further S. mutans colonization （5 minutes treatment： 38 ± 13 fold reduction P=0.01; 16 hours treatment： 96 ± 28 fold reduction P=0.07）. Conclusion S. mutans infection is reduced by the pre- sence of existing biofilms. Thus maintaining a healthy or ＂normal＂ biofilm through targeted antimicrobial therapy （such as the STAMPs） could represent an effective strategy for the treatment and prevention of S. mutans colonization in the oral cavity and caries progression.

关键词： targeted antimicrobial therapy antimicrobial peptide biofilm streptococcus mutans protective colonization caries

In silico identification of potential inhibitors targeting streptococcus mutans sortase A

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International Journal of Oral Science 2017年第1期9卷 53-62页

作者： Hao Luo Dan-Feng Liang Min-Yue Bao Rong Sun Yuan-Yuan Li Jian-Zong Li Xin Wang Kai-Min Lu Jin-Ku Bao School of Life Sciences and Key Laboratory of Ministry of Education for Bio-Resources and Bio-Environment Sichuan University State Key Laboratory of Biotherapy/Collaborative Innovation Centre for Biotherapy West China HospitalSichuan University State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan University

Dental caries is one of the most common chronic diseases and is caused by acid fermentation of bacteria adhered to the teeth. streptococcus mutans （S. mutans） utilizes sortase A （SrtA） to anchor surface proteins to the cell wall and forms a biofilm to facilitate its adhesion to the tooth surface. Some plant natural products, especially several flavonoids, are effective inhibitors of SrtA. However, given the limited number of inhibitors and the development of drug resistance, the discovery of new inhibitors is urgent. Here, the high-throughput virtual screening approach was performed to identify new potential inhibitors of S. mutans SrtA. Two libraries were used for screening, and nine compounds that had the lowest scores were chosen for further molecular dynamics simulation, binding free energy analysis and absorption, distribution, metabolism, excretion and toxicity （ADMET） properties analysis. The results revealed that several similar compounds composed of benzofuran, thiadiazole and pyrrole, which exhibited good affinities and appropriate pharmacokinetic In addition, the carbonyl of these compounds can have a strategy for microbial infection disease therapy. parameters, were potential inhibitors to impede the catalysis of SrtA. key role in the inhibition mechanism. These findings can provide a new

关键词： dental caries molecular dynamics simulation molecular docking potential inhibitors sortase A streptococcus mutans