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streptococcus agalactiae:Sensitivity profile in pregnant women attending health units in northeastern Brazil

World Journal of Obstetrics and Gynecology 2020年第1期9卷 11-17页

作者： Tais Viana Ledo de Oliveira Fabrícia Almeida Fernandes Santana Caline Novais Teixeira Oliveira Maria Luísa Cordeiro Santos Fabrício Freire de Melo Cláudio Lima Souza Márcio Vasconcelos Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

BACKGROUNDG roup B streptococcus agalactiae (GBS) is the main etiologic agent associated withearly-onset neonatal sepsis, and of all newborns of parturients colonized by GBS,approximately 1%-2% develop invasive, early-onset disease. The risk of infectionincreases to 15.2% in premature neonates, to 10.7% when the parturient haschorioamnionitis or premature rupture of membranes for more than 24 h and to9.7% if the mother has postpartum bacteremia. In addition to causing perinatal,neonatal and postnatal deaths, neonatal hospital infection is associated with highcosts, as hospitalization is three times longer than in uninfected children. Theidentification of pregnant women colonized by GBS, through universal screening,associated with the adoption of appropriate antibiotics at the time of delivery arethe most successful preventive *** evaluate the sensitivity profile of GBS isolated from pregnant womenattending Vitória da *** This is a cross-sectional study with a quantitative approach carried out in themunicipality of Vitória da Conquista-Bahia between February 2017 and March2018. The study population was composed of 210 pregnant women, with agestational age of 32 to 40 wk, who were aged 18 years or older living in the urbanarea of the municipality of Vitória da Conquista. After a brief explanation aboutthe research and obtaining a signed an informed consent form, data andvaginorectal swabs were collected from the women for GBS research. Examinationof the samples in order to identify the presence of GBS was by culture on sheep blood agar and chromogenic agar for GBS and then, seeded on plates containingstreptococcal culture medium and incubated for 18 h to 24 h at 35°C. Theantimicrobial sensitivity profile of positive GBS samples was determined by thedisk diffusion technique, according to the Clinical and Laboratory StandardsInstitute manual (2017). The data obtained were stored in a database usingMicrosoft Office Excel spreadsheets and a des

关键词： streptococcus agalactiae Sensitivity profile Pregnancy Clindamycin Erythromycin Group B streptococcal disease

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Sequential One-Pot Three-Enzyme Synthesis of the Tetrasaccharide Repeating Unit of Group B streptococcus Serotype Ⅷ Capsular Polysaccharide

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Chinese Journal of Chemistry 2022年第9期40卷 1039-1044页

作者： Min Liang Wei Gong Chongzhen Sun Jielin Zhao Hong Wang Zonggang Chen Min Xiao Guofeng Gu National Glycoengineering Research Center Shandong Provincial Key Laboratory of Carbohydrate Chemistry and GlycobiologyShandong University72 Binhai RoadQingdaoShandong 266237China School of Pharmaceutical Science Shandong University44 West Wenhua RoadJinanShandong 250012China NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-based Medicine Shandong UniversityQingdaoShandong 266237china

The biochemical property and functional identification of three recombinant glycosyltransferases,includingβ-1,4-rhamnosyltransferase(Cps8R),β-1,4-galactosyltransferase(Cps8) and α-2,3-sialyltransferase(Cps8K)involved in the biosynthesis of the tetrasaccharide repeating unit of serotypeⅧcapsular polysaccharide(CPS)of Group B Streptococci(GBS),were systematically ***,these recombinant enzymes were employed for one-pot three-enzyme efficient synthesis of the tetrasaccharide repeating unit of GBS serotypeⅧCPS using the chemically synthesized Glca-PP-(CH2)ir-OPh as the starting substrate in a satisfying yieldof 87%.

关键词： streptococcus agalactiae Capsular polysaccharide Oligosaccharides Enzyme catalysis Biosynthesis

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Distribution and Prevalence of Serotypes of Group B streptococcus Isolated from Pregnant Women in 30 Countries:A Systematic Review

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Maternal-Fetal Medicine 2023年第2期5卷 97-103页

作者： Marta Maria Silva Érica Alcântara Silva Caline Novais Teixeira Oliveira Maria Luísa Cordeiro Santos Cláudio Lima Souza Fabrício Freire de Melo Márcio Vasconcelos Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaBahia 45.029-094Brazil

Objective:This review aimed to compile scientific data on the distribution and prevalence of group Bstreptococcus (GBS) serotypes isolated from pregnant women across 30 countries from 2010 to ***:This was a systematic review that addresses the distribution and prevalence of GBS in pregnant *** search included studies published between January 2010 and December 2019 in PubMed,Virtual Health Library (BVS),ScienceDirect,Scientific Electronic Library Online (SciELO),and LILACS *** also surveyed relevant articles published in English,Spanish,and Portuguese between February and April *** articles,communication,short report,theses,and dissertations were *** prevalence of GBS colonization,method for capsular serotyping,antimicrobial resistance,distribution and prevalence of serotypes were extracted from each ***:In all,795 publications were *** applying the eligibility criteria,48 articles were included for the final systematic analysis;most articles were from Asia and were published during the years 2014 to *** the identification of serotypes,most studies used the polymerase chain reaction *** were records of all 10 GBS serotypes,namely,Ia,Ib,and II–IX,among the countries *** susceptibility and resistance to antibiotics were addressed in 37.5% of the publications ***:This review showed that GBS serotypes are distributed differently in the 30 analyzed countries,with serotypes Ia,Ib,and II to V being the most ***,our results highlighted the relationship of GBS with maternal colonization,implications for neonates,and antibiotic resistance.

关键词： streptococcus agalactiae Serotype Pregnant women

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The relationship between sexually transmitted microorganisms and seminal quality in asymptomatic men

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Asian Journal of Urology 2022年第4期9卷 473-479页

作者： Valentina Velasquez Rivera Walter D.Cardona Maya Jenniffer Puerta-Suarez Department of Microbiology and Parasitology Medical SchoolUniversity of AntioquiaAntioquiaColombia

Objective:To detect DNA of different microorganisms,in semen samples from apparently healthy men and correlate their presence with seminal ***:Semensamples from 81 healthy volunteers were collected,and semen parameters were *** extraction was performed using the phenol-chloroform technique,and the micro-organisms were detected by the amplification of specific primers using polymerase chain ***:DNA from at least one of the microorganisms was detected in 78 *** most frequent microorganism found in semen were:Lactobacillus spp.(70%),Neisseria gonorrhoeae(***)(36%),streptococcus epidermidis(64%),Klebsiella pneumoniae(56%),Staphylococcus aureus(32%),Chlamydia trachomatis(***)(28%),Pseudomonas aeru-ginosa(27%).The seminal parameters of all semen samples were over the lower reference values fornormal *** compare with negative samples,seminal volume was higher for the Escherichia coli positive samples and lower for Pseudomonas aeruginosa positive *** samples positive for Staphylococcus aureus had worse sperm *** frequency of progressive motility was higher in positive samples for *** and *** semen samples for *** had a higher concentration per ***:It is common to find microorganisms in semen of asymptomatic men,including those responsible for sexually transmitted *** treatment is recommended only in those individuals with a sexually transmitted infection(*** and ***)and always promote condom use.

关键词： Chlamydia trachomatis Neisseria gonorrhoeae Enterococcus faecalis streptococcus agalactiae Staphylococcus spp. Seminal quality

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In Vitro Antibacterial Effect of Traditional Chinese Medicine on Main Pathogenic Bacteria of Recessive Mastitis in Dairy Cows

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Animal Husbandry and Feed Science 2017年第1期9卷 34-36页

作者： Gao Guisheng Hu Tiefeng An Jiwei Gao Guangping Shi Qiumei Shao Xinhua Zhang Yanying Hebei Provincial Key Laboratory of Veterinary Preventive Medicine Hebei Normal University of Science and TechnologyChangli 066600China Animaland Veterinary Bureau of Laoting County Tangshan 063600China Hebei Xinhua Veterinary Drug Co. Ltd.Luancheng 051430China

The inhibition zone diameter, minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of 20 kinds of traditional Chi- nese medicine on Staphylococcus aureus and streptococcus agalactiae isolated from recessive mastitis were studied using plate test and tube test. The results showed that S. aureus and S. agalactiae were sensitive to all these 20 kinds of traditional Chinese medicine, and were more sensitive to seven kinds of traditional Chinese medicine, including Taraxtwum mongolicum, Coptis chinensis, Forsythia suspense, Andrographis paniculata, Carthamus tinctorius, Scutellaria baicalensis and Den- dranthema indicum.

关键词： Traditional Chinese medicine streptococcus agalactiae Inhibition zone diameter MIC MBC

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