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The　technique　of　double─stranded　RNA：a

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浙江大学学报(农业与生命科学版) 1996年第3期 111-112页

作者：阮红李德葆浙江农业大学生物技术研究所浙江大学生物科学与技术系

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关键词：李德 RNA stranded The technique double 杭州浙江双链

PREDICTION FOR FOLDING STRUCTURE OF THE MAXIMUM BASE PAIRS OF SINGLE-stranded RNA (DNA)

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Chinese Science Bulletin 1984年第12期29卷 1679-1684页

作者：乐树云江寿平 Shanghai Institute of Biochemistry Academia Sinica

A native RNA is a single polynucleotide strand. It has been shown by X-ray diffraction analysis data that a single-stranded RNA can be folded to double helical structure in part by hydrogen bonds between pairing bases (mainly A-U and G-C) and to form loop region in part between unpaired bases. So the high structure of proper alternate arrangement of helical and loop structure can be formed in a single-

关键词： helical alternate stranded RNA pairing bonds bases folded vertex subgraph

Computational Viromics: Applications of the Computational Biology in Viromics Studies

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Virologica Sinica 2021年第5期36卷 1256-1260页

作者： Congyu Lu Yousong Peng Bioinformatics Center College of BiologyHunan Provincial Key Laboratory of Medical VirologyHunan UniversityChangsha 410082China

Viruses are a kind of biological entities which rely on host cells for *** on the genetic materials and replication mode,they can be grouped into double-stranded DNA(dsDNA),single-stranded DNA(ssDNA),doublestranded RNA(dsRNA),positive-sense single-stranded RNA(+ssRNA),negative-sense single-stranded RNA(-ssRNA),ssRNA reverse transcriptase viruses(ssRNART)and dsDNA reverse transcriptase viruses(dsDNA-RT)(Walker et al.2020).Viruses can infect most kinds of biological entities,including viruses,bacteria,archaea and eukaryote(La Scola et al.2008;Fermin,2018).

关键词： al. dsDNA stranded

Generation of pigs with a Belgian Blue mutation in MSTN using CRISPR/Cpf1-assisted ssODN-mediated homologous recombination

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Journal of Integrative Agriculture 2019年第6期18卷 1329-1336页

作者： ZOU Yun-long LI Zhi-yuan ZOU Yun-jing HAO Hai-yang HU Jia-xiang LI Ning LI Qiu-yan State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest UniversityChongqing 400715P.R.China State Key Laboratory for Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijing 100193P.R.China College of Veterinary Medicine China Agricultural UniversityBeijing 100193P.R.China

CRISPR/Cpf1 has emerged recently as an effective tool for genome editing in many organisms,but its use in pigs to generate precise genetic modifications has seldom been ***(MSTN)is a well-characterized negative regulator of muscle development,and natural mutations in this gene cause a double-muscled phenotype in many ***,to the best of our knowledge,no naturally occurring mutation in MSTN has been found in *** addition,no living pig models with sophisticated modifications orthologous to natural mutations in MSTN have yet been *** this study,we exploited the CRISPR/Cpf1 system to introduce a predefined modification orthologous to the natural MSTN mutation found in Belgian Blue cattle(thus known as the Belgian Blue mutation).Our research demonstrated that the cutting efficiency of CRISPR/Cpf1 was 12.3%in mixed porcine fetal fibroblasts in drug free medium,and 41.7%in clonal colonies obtained using G418 ***,the Cpf1-sgRNA vector,ssODN template,and a self-excision cassette were co-transfected into porcine fetal *** G418 selection,8 clonal colonies were examined and 5 with genetic modification were *** these 5,2 harbored the precise 11-bp *** 1 heterozygous clonal colony,2 cloned Duroc piglets were successfully generated,which was heterozygous for the Belgian Blue *** summary,our results demonstrate that CRISPR/Cpf1 system can be used efficiently to generate double-stranded breaks,and also to mediate homologous recombination to introduce precise genomic modifications in pigs.

关键词： MSTN CRISPR/Cpf1 Belgian Blue mutation genetically modified pigs single stranded oligodeoxynucleotide

Identification of a novel hepacivirus in Mongolian gerbil(Meriones unguiculatus)from Shaanxi,China

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Virologica Sinica 2022年第2期37卷 307-310页

作者： Cui-hong An Juan Li Yi-ting Wang Shou-min Nie Wen-hui Chang Hong Zhou Lin Xu Yang-xin Sun Wei-feng Shi Ci-xiu Li Department of Plague and Brucellosis Shaanxi Center for Disease Control and PreventionXi’An 710054China Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan250021China Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong Shandong First Medical University&Shandong Academy of Medical SciencesTaian271000China Department of Pathogen Biology School of Basic MedicineShandong First Medical University&Shandong Academy of Medical SciencesTaian271000China Shaanxi Center for Disease Control and Prevention Xi’An710054China School of Public Health Shandong First Medical University&Shandong Academy of Medical SciencesTaian271000China

Dear Editor,Hepaciviruses,members of the family Flaviviridae,are enveloped viruses containing a single-stranded positive-sense RNA genome of approximately 8.9-10.5 kb in size(Simmonds et al.,2017).To date,15species(Hepacivirus A-N,and P)have been documented within the Hepacivirus genus that show distinct host ranges,including primates,bats,horses,donkeys,cows,and various rodents(Hartlage et al.,2016).

关键词： unguiculatus stranded envelope

Characterization of an infectious pancreatic necrosis virus from rainbow trout fry(Onhorhynchus mykiss) in West Ukraine

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Virologica Sinica 2015年第3期30卷 231-233页

作者： Yuriy P Rud Maria I Maistrenko Leonid P Buchatskiy Laboratory of Biotechnology in Aquaculture Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine Department of Molecular Biology Biotechnology and Biophysics Institute of High Technologies Taras Shevchenko National University of Kyiv Laboratory of Physico-Chemical Biology ESC Institute of BiologyTaras Shevchenko National University of Kyiv

Dear Editor,Aquatic birnaviruses are a group within the family Birnaviridae that comprise isolates from fish and shellfish from both fresh-and seawater(Woo and Bruno,1999).Members of the family Birnaviridae are icosahedral viruses approximately 60 nm in diameter composed of five polypeptides and two segments of double-stranded RNA(Dixon et al.,2008).In this report,an aquatic birnavirus was isolated from rainbow trout fry,Onhorhynchus myki-

关键词： trout shellfish infectious Dixon isolates Bruno aquatic stranded fresh comprise

RNA-Directed DNA Methylation Links Viral Disease and Plant Architecture in Rice

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Molecular Plant 2020年第6期13卷 814-816页

作者： Taiji Kawakatsu Institute of Agrobiological Sciences National Agriculture and Food Organization3-1-3 KannondaiTsukubaIbaraki 305-8604Japan

Cytosine methylation is an epigenetic modification of DNA that can affect gene expression and silence transposable element(TE)*** plants,cytosine methylation occurs in three distinct sequence contexts:CG,CHG,and CHH(where H=C,A,or T)(Law and Jacobsen,2010).The RNA-directed DNA methylation(RdDM)pathway catalyzes all types of de novo cytosine methylation,especially within small TEs or at the edges of large *** brief,two plant-specific RNA polymerases,Pol IV and Pol V,independently synthesize relatively short single-stranded RNAs at RdDM target regions(Law and Jacobsen,2010).Pol IV transcripts are converted to double-stranded RNA by RNA-DEPENDENT RNA POLYMERASE *** double-stranded RNAs are processed into 24-nt small interfering RNAs(siRNAs)by DICER-LIKE *** 4 binds these siRNAs and is targeted to RdDM target loci,with Pol V transcripts as ***,DOMAINS REARRANGED METHYLTRANSFERASE 2 is recruited to RdDM target regions through an indirect interaction with ARGONAUTE 4 and catalyzes cytosine methylation.

关键词： Plant stranded Jacobsen

Molecular Characterization of Human Respiratory Adenoviruses Infection in Xining City, China In 2018

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Virologica Sinica 2021年第3期36卷 545-549页

作者： Juan Yu Shengcang Zhao Huaxiang Rao Department of Basic Medical Sciences Changzhi Medical CollegeChangzhi 046000China Department of Public Health and Preventive Medicine Changzhi Medical CollegeChangzhi 046000China Center of Hygiene Inspection Qinghai Center for Disease Control and PreventionXining 810007China Institute for Communicable Disease Control and Prevention Qinghai Center for Disease Control and PreventionXining 810007China

Dear Editor,Adenoviruses are non-enveloped,double-stranded DNA viruses belonging to the Adenoviridae *** date,100unique genotypes of adenoviruses have been identified,from species A to G,could cause respiratory tract,... 详细信息

关键词： Human stranded envelope

New prevalence estimate of Torque Teno virus(TTV) infection in healthy population and patients with chronic viral hepatitis in Jiujiang, China

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Virologica Sinica 2015年第3期30卷 218-220页

作者： Jin Peng Yueyue Fang Xuesen Zhao Yihong Peng Jiujiang Women's & Children's Hospital Department of Microbiology School of Basic Medical SciencePeking University

Dear Editor,Torque Teno virus(TTV)is a nonenveloped human DNA virus that was isolated from the serum of a patient with transfusion-transmitted hepatitis with unknown etiology in 1997(Nishizawa et al.,1997).TTV is the first human virus with a single-stranded circular DNA genome to be identified,and is recently classified as the Alphatorquevirus genus of the Anelloviridae family by the International Committee on Taxonomy of Viruses(ICTV)(King et al.,2011).TTV shows very high genetic

关键词： prevalence DNA transfusion circular classified etiology stranded transmitted comparable serological