检索结果-南通市图书馆

Unlocking a new target for streptomycetes strain improvement

Synthetic and Systems Biotechnology 2020年第1期5卷 33-34页

作者： Yuan Chen Jackie Metz RenéK.Miller-Xavier Guojun Wang Harbor Branch Oceanographic Institute Florida Atlantic University5600 U.S.1Fort PierceFlorida34946United States Research Institute of Agri-engineering Technology Fujian Academy of Agricultural SciencesFuzhou350003China

Bacteria of the Streptomyces genus are well-known producers of secondary metabolites of high medical *** contributed nearly 60%of current antibiotics(i.e.,vancomycin,daptomycin and tetracycline),as well as antifungals,antiparasitics(avermectins),anticancer drugs(doxorubicin),immunosuppressants and others[1].Therefore,in the past several decades,Streptomyces bacteria or the therein involved biosynthetic pathways have long been the central topic of strain improvement,metabolic engineering,and *** of this research has focused on regulatory elements;however,it has been a challenge due to the complex regulatory network that controls Streptomyces secondary metabolism and its complex fungus-like morphological differentiation[2].On agar plates,production of secondary metabolites coincides with the switch from vegetative growth(substrate mycelium)to aerial mycelium hyphae and subsequent spore formation,whereas in a liquid culture,early stationary phase when cells stop growing marks the initiation of secondary metabolism.

关键词： Polyketide Streptomyces strain improvement Triacylglycerol β-oxidation Acetyl-CoA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain

引用

Frontiers of Chemical Science and Engineering 2012年第2期6卷 174-178页

作者： Jixian GONG Nan DUAN Xueming ZHAO School of Textiles Tianjin Polytechnic UniversityTianjin 300160China School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China

Evolutionary engineering is a novel whole- genome wide engineering strategy inspired by natural evolution for strain improvement. Astaxanthin has been widely used in cosmetics, pharmaceutical and health care food due to its capability of quenching active oxygen. strain improvement ofPhaffia rhodozyma, one of the main sources for natural astaxanthin, is of commercial interest for astaxanthin production. In this study a selection procedure was developed for adaptive evolution of P. rhodozyma strains under endogenetic selective pressure induced by additive in environmental niches. Six agents, which can induce active oxygen in cells, were added to the culture medium respectively to produce selective pressure in process of evolution. The initial strain, P. rhodozyma AS2-1557, was mutagenized to acquire the initial strain population, which was then cultivated for 550 h at selective pressure and the culture was transferred every 48h. Finally, six evolved strains were selected after 150 generations of evolution. The evolved strains produced up to 48.2% more astaxanthin than the initial strain. Our procedure may provide a promising alternative for improvement of highproduction strain.

关键词： evolutionary engineering astaxanthin strain improvement

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Remarkable enhancement of bleomycin production through precise amplification of its biosynthetic gene cluster in Streptomyces verticillus

引用

Science China(Life Sciences) 2022年第6期65卷 1248-1256页

作者： Hong Li Wenyan Gao Yifan Cui Yuanyuan Pan Gang Liu State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100864China

Amplification of biosynthetic gene clusters is important to increase secondary metabolite ***,the copy number of amplified gene clusters is difficult to control *** this study,the tandem amplification of a 70 kb bleomycin biosynthetic gene cluster was precisely regulated through the combined strategy of a Zou A-dependent DNA amplification system and double-reporter-guided recombinant selection in Streptomyces verticillus *** production of bleomycin in the recombinant strain containing six copies of the bleomycin gene cluster was 9.59-fold higher than that in the wild-type *** combined strategy used in this study is powerful and applicable for precisely regulating the amplification of gene clusters and improving the corresponding secondary metabolite production.

关键词： bleomycin precise regulation amplification of gene cluster strain improvement

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Improved production of spiramycin by mutant Streptomyces ambofaciens

引用

Journal of Zhejiang University Science 2004年第6期5卷 689-695页

作者：金志华岑沛霖 DepartmentofBiologicalandPharmaceuticalEngineering NingboInstituteofTechnologyZhejiangUniversityNingbo315100China InstituteofBioengineering CollegeofMaterialsScienceandChemicalEngineeringZhejiangUniversityHangzhou310027China

strain improvement and medium optimization to increase the productivity of spiramycin were carried out. Of oil tolerant mutant strains screened, one mutant, Streptomyces ambofaciens XC 2-37, produced 9% more spiramycin than the parent strain S. ambofaciens XC 1-29. The effects of soybean oil and propyl alcohol on spiramycin production with S. ambofaciens XC 2-37 were studied. The potency of S. ambofaciens XC 2-37 was improved by 61.8% with addition of 2% soybean oil in the fermentation medium and 0.4% propyl alcohol at 24 hours after incubation. The suitable time for feeding propyl alcohol is at 24 hours after incubation in flask fermentation and at 20 hours after incubation in fermentor fermentation. The new process with S. ambofaciens XC 2-37 was scaled up for industrial scale production of spiramycin in a 60 m3 fermentor in Xinchang Pharmaceutical Factory, Zhejiang Medicine Company, Ltd., China, and the potency and productivity of fermentation were improved by 42.9%.

关键词： Spiramycin Streptomyces ambofaciens strain improvement Fermentation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Regulation of antibiotic biosynthesis in actinomycetes:Perspectives and challenges

引用

Synthetic and Systems Biotechnology 2018年第4期3卷 229-235页

作者： Junhong Wei Lang He Guoqing Niu Biotechnology Research Center Southwest UniversityChongqing400715China State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqing400715China Academy of Agricultural Sciences Southwest UniversityChongqing400715China

Actinomycetes are the main sources of *** onset and level of production of each antibiotic is subject to complex control by multi-level *** regulators exert their functions at hierarchical *** the lower level,cluster-situated regulators(CSRs)directly control the transcription of neighboring genes within the gene ***-level pleiotropic and global regulators exert their functions mainly through modulating the transcription of *** in understanding of the regulation of antibiotic biosynthesis in actinomycetes have inspired us to engineer these regulators for strain improvement and antibiotic discovery.

关键词： Antibiotic biosynthesis Regulation Actinomycetes Genetic manipulation strain improvement Antibiotic discovery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：