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The storability and Its Regulatory Mechanism of HuanghuaPear(Pyrus pyrifolia Nakai.)Fruit as Influencedby Postharvest Treatments

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Agricultural Sciences in China 2002年第11期1卷 1238-1245页

作者： DOU Shi-juan, CHEN Kun-song, LU Jun-liang and ZHENG Jin-tu(Laboratory of Fruit Molecular Physiology and Biotechnology , Zhejiang University , Hangzhou 310029 ,P. R. China Ningbo Forestry Bureau, Ningbo 315010 ,P. R. China) Laboratory of Fruit Molecular Physiology and Biotechnology Zhejiang University Hangzhou 310029 P.R.China Ningbo Forestry Bureau Ningbo 315010P.R.China

Different temperatures and PEF packing treatments were carried out on postharvest Huanghua pear fruit to investigate their effects on fruit storability and the regulatory mechanism. LOX activity, O2- content, AOS activity, ACC synthase activity, ACC content, ACC oxidase activity and ethylene production changed with peaks in the ripening fruit at 20℃ and were inhibited by cold storage, incidence of fruit woolness and fruit decay were lightened as well. Low temperature combined with PEF packing (PEF1 and PEF2) treatments could further improve the fruit storability, maintain preferable quality. There was no significant difference between PEF1 and PEF2 both during cold storage at 1℃ and shelf life at 20℃. The recommended storage period of Huanghua fruit was two months at It and could be extended one month longer with PEF packing treatments.

关键词： Huanghua pear fruit Ripening Senescence PEF storability Mechanism Regulation

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The Status and Strategy on the Relationship Between Lipoxygenase-3 in Rice(Oryza sativa L.) Embryos and storability

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Agricultural Sciences in China 2002年第7期1卷 798-804页

作者： SHEN Wen-biao, ZHENG Tian-qing, ZHAI Hu-qu and WAN Jian-min( State Key Laboratory for Crop Genetics and Germplasm Enhancement /Rice Research Institute, Nanjing Agricultural University, Nanjing 210095 ,P.R. China ) State Key Laboratory for Crop Genetics and Germplasm Enhancement/Rice Research Institute Nanjing Agricultural University Nanjing 210095 P.R.China

The degradation of lipids was mainly responsible for the reduction of rice quality and production of stale flavor during its storage. It was proved that LOX-3, the major component of the isozymes accounting for 80% - 90% of total activity, was the key enzyme in above process. Further results indicated that the lack of LOX-3 in Daw Dam led to decrease of lipid peroxidation, alleviating the accumulation of stale flavor during storage, which is caused by hexanal, pentanal and pentanol compounds etc. Genetic survey displayed that the absence of LOX-3 was controlled by a single Mendelian recessive gene. On the other hand, three cloned LOX genes and deduced LOX genes encoding some purified LOXs could not be contributed to the embryo LOX genes by comparing biochemical characterizations of these expressed products and some purified LOXs with known LOXs in rice embryos. The future research should focus on the further influences of LOX-3-null on rice storability. Meanwhile, single-base-trans version in coding region, insertion, deletion and replacement in promoter of LOX genes were related with lipoxygenase-null genotype of soybean and pea. Therefore, the cloning of the recessive gene controlling the null of LOX-3 and its corresponding dominant gene in rice seed embryos will contribute to the understanding of the null molecular mechanism, elucidating the differences of LOX genes expression and regulation in rice embryos. In the last, the breeding strategy of new storable rice, including the theoretical and practical concepts, was surveyed.

关键词： Rice (Oryza saliva) Embryo Lipoxygenase-3 storability

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Viability of recalcitrant Araucaria angustifolia seeds in storage and in a soil seed bank

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Journal of Forestry Research 2020年第6期31卷 2413-2422页

作者： Ezequiel Gasparin Jose M.R.Faria Anderson CJose Olivia AOTonetti Rodrigo Ade Melo Henk W.M.Hilhorst Departamento de Ciencias Florestais Universidade Federal de Santa Maria(UFSM)Avenida Roraima1000CamobiSanta MariaRS 97105-900Brazil Departamento de Ciencias Florestais Universidade Federal de Lavras(UFLA)Caixa Postal 3017LavrasMGBrazi1 Wageningen Seed Lab Laboratory of Plant PhysiologyWageningen UniversityDroevendaalsesteeg 16708 PB WageningenThe Netherlands

Araucaria angustifolia(Bertol.)Kuntze is a representative species of the Mixed Ombrophilous Forest in the Atlantic Forest Biome of *** development of a germplasm conservation protocol for long-term seed bank storage is compromised for this species,as it is sensitive to ***,in situ establishment of a soil seed bank in its natural habitat may be *** study evaluates the storability of two provenances of *** seeds and their behavior in an artificial soil seed bank in two forest environments(understory and edge).Results show that both seed provenances may be stored at 5C for approximately 12 months,retaining high *** subsequent decrease in germination was associated with a reduction and an increase in seed water content,as well as with increased electrical *** the understory environment,seed viability was above 85%for the first 60 days,and at the end of the experiment(270 days),seedlings ***,at the forest edge,there was a total loss of seed viability after 120 days associated with a reduction in water content and high *** is concluded,therefore,that short-term storage of *** seeds is possible in a cold room,which is fundamental to supply seed demand outside the production *** cover conservation is important for regeneration and conservation of the species.

关键词： Brazilian pine Desiccation sensitivity Forest cover Soil seed bank storability

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The Seed Water Sorption Isotherm and Antioxidant-Defensive Mechanisms of <i>Hordeum vulgare</i>L. Primed Seeds

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American Journal of Plant Sciences 2018年第12期9卷 2385-2407页

作者： Pinpinatt Junhaeng Pitipong Thobunluepop Wanchai Chanprasert Damrongvudhi Onwimol Sutkhet Nakasathien Elke Pawelzik Department of Agronomy Faculty of Agriculture Kasetsart University Bangkok Thailand Division of Quality of Plant Products Department of Crop Sciences Faculty of Agricultural Sciences Georg-August-Universit&auml t Gö ttingen G&ouml ttingen Germany

The key successful for seed priming technique was the management of seed imbibition process. This study was aimed to indicate the seed moisture sorption characteristics of Hordeum vulgare L. cv. Samerng 2 in relation to the seed priming technique and its effect on the seed qualities. The experiment was designed by Completely Randomized Design (CRD) with four replications. Seed hydro-priming technique was done under 25 degree Celsius, and 40% - 60% RH. Barley seed moisture content was determined every hour after imbibition for 24 hours. Then, primed barley seed qualities were evaluated at 6, 8, 10, 12, 14, and 16 hours of hydrotime. The results revealed that barley seeds were imbibed rapidly from 10.55% to 37.69% seed moisture content during 0 - 9 hours of imbibition. The constantly seed moisture content was continued until 17 hours of imbibition. Radicle was protruded with 42.74% seed moisture content. Seed moisture sorption characteristic equation was Y = 0.0055x3- 0.267x2 + 4.5931x + 13.348. Coefficient of determination (R2) was 0.916. Primed seed germination was not affected by hydrotime. Primed seeds were improved in speed of germination and seedling growth rate. The best primed barley seed vigorous was noted at 14 - 16 hours of hydrotime. In addition, catalase activity (CAT) and superoxide dismutase activity (SOD) were not significantly different for non-primed and 14 - 16 hours primed seeds. However, lipoxygenase activity (LOX) was increased and malondialdehyde (MDA) content was decreased for 14 - 16 hours primed seeds. Subsequently, 14 - 16 hours primed barley seeds can maintain high seed germination and vigor for 8 - 12 months under 5 degree Celsius. Thus, it could be concluded that hydro-priming for 14 - 16 hours enhances barley seed quality by reducing germination time, induced defenses mechanism, and could be stored for 8 - 12 months.

关键词： Seed Moisture Sorption Characteristic Barley Seed Hydro-Priming Seed Germination Speed of Germination storability Defenses Mechanism

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Feasibility of using polypropylene ground cover upon consideration of long-term responses of sweet cherry nutrition and profitability

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Agricultural Sciences 2012年第4期3卷 594-601页

作者： Xinhua Yin Clark F. Seavert Assistant Professor Department of Plant Sciences The University of Tennessee 605 Airways Blvd. Jackson TN 38301 Department of Agricultural and Resource Economics Ballard Extension Hall Oregon State University Corvallis OR 97331

The impacts of synthetic polypropylene ground cover in the row area of sweet cherry (Prunus avium L.) trees (‘Regina’/’Gisela 6’) on soil nutrient availability, tree mineral nutrition and productivity, and economic returns were investigated on a Van Horn fine sandy loam soil at Hood River OR, from 2000 to 2007. Treatments included 2.44-m wide synthetic fabric ground cover made of black, woven poly-propylene over the row area of cherry trees (woven fabric), and no ground cover but with herbicide applications in the row area with the same width as the polypropylene ground cover (herbicide strip) – standard industry practice. This article reports the plant nutrition and soil fertility results of 2006 and 2007 and profitability and feasibility results of 2000 to 2007. Tree leaf nitrogen (N) concentrations were significantly higher with 9 to 14% increases using woven fabric compared with herbicide strip in 2006 and 2007. However, leaf sulfur (S) concentrations were significantly lowered with woven fabric in the two seasons. Woven fabric resulted in fruit with comparable quality and possible greater storability under enhanced fruit yields than herbicide strip in both years. Woven fabric was more profitable than herbicide strip based on an additional net present value of $2,606 ha-1 by the end of this study. Woven fabric had annual gross returns greater than annual costs in the fourth year after planting by $8,181 ha-1 relative to herbicide strip, and had cumulative net returns greater than total costs of all previous years in the sixth year after planting by $17,796 ha-1 over herbicide strip. However, to establish a sweet cherry orchard with woven fabric, the grower would spend an additional $4,332 ha-1 over herbicide strip. In conclusion, woven fabric is a profitable and sustainable in-row ground management alternative to herbicide strip for orchards from a long-term perspective.

关键词： Polypropylene Ground Cover Sweet Cherry Leaf Nutrition Fruit storability Annual Gross Returns Annual Cost Annual Net Returns Cumulative Net Returns Present Value

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