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Apparent Densities of stomoxys Species (Diptera, Muscidae) of Different Physiological Ages Caught with Vavoua Trap Differ With Landscape and Trapping Period

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Journal of Zoological Research 2021年第1期3卷 9-14页

作者： Sevidzem S.Lendzele Koumba A.Aubin Zinga-Koumba C.Roland Mintsa-Nguema Rodrigue Jacques F.Mavoungou Ecole Doctorale des Grandes Ecoles(EDGE) LibrevilleGabon Laboratoire d’Ecologie Vectorielle(LEV-IRET) LibrevilleGabon Institut de Recherche en Ecologie Tropicale(IRET) BP 13354LibrevilleGabon Universitédes Sciences et Techniques de MASUKU BP 941FrancevilleGabon

Stable flies(stomoxys spp.)are common pests of livestock in the peri-urban rangelands of Vina Division of the Adamawa *** documented information is available on their diurnal dynamics in relation to physiological age and *** main aim of this study was to determine the trap apparent density(ADT)of stomoxys and associate it to their diurnal activity rhythm and *** traps(n=12)were used for stomoxys *** was carried out for seven days *** days consecutively in August and four days consecutively in October,*** traps were pitched in each of the three biotopes(river,cattle corral and gallery forest)of the four study sites(Galim,Mbidjoro,Velambai and Soukourwo).All female stomoxys captured underwent ovarian dissection for the determination of their physiological ages(parous or nulliparous).A total of 218 stomoxys were caught and identified into four species(*** niger,***,***,and ***)with an overall ADT of 2.59 flies per trap per day and stomoxys niger niger(1.13 *** trap per day)recorded the highest *** richness was study site *** catches of females 113(51.83%)were made than that of their male 105(48.17%)*** recorded the highest stomoxys apparent density(4.90)as compared to other sites with a statistically significant difference(P<0.05).The proportion of parous 87(59.30%)was higher than that of their nulliparous 46(40.71%)*** differed with landscape and capture *** was the most frequent in all biotopes and Galim recorded the highest *** population of female flies was made up of adults(parous)with a bimodal diurnal activity rhythm whereas males had a unimodal activity rhythm.

关键词： Abundance Parity Landscape Trapping period stomoxys Vina Cameroon

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Impact of Manure Storage Time and Temperature on Microbial Composition and Stable Fly (Diptera: Muscidae) Development

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Advances in Microbiology 2019年第3期9卷 248-265页

作者： Janice L. Speshock Jeff A. Brady Jessica Eastman Travis Roach Samantha Hays David Kattes Department of Biology Tarleton State University Stephenville USA Department of Wildlife Sustainability & Ecosystem Sciences Tarleton State University Stephenville USA Texas A&M AgriLife Research & Extension Center Stephenville USA Department of Agriculture & Environmental Science Tarleton State University Stephenville USA

Samples are often frozen for preservation until needed for use. It has been a common practice to store fresh dairy manure in the freezer until needed for fly development studies. However, conflicting data have suggested that freezer temperature and duration of manure may impact fly development studies, and it is likely due to the change in microbial comminutes due to the freezer conditions. In this study manure storage conditions were assessed to ascertain how temperatures impact stable fly, stomoxys calcitrans L., survival to pupation and determine which bacterial populations impacted fly development using massively-parallel sequencing and 16S metagenomic analysis. Stable fly survival to pupation was greater in manure that was stored warm (27?C) or frozen (-20?C or -80?C) for 24 days as compared to fresh manure samples. Refrigeration (4?C) of the manure for 24 days did not affect fly development and slightly decreased the pupal weights. Over 80 bacterial families were detected by sequencing allowing for a more thorough assessment of changes in bacterial populations. Only minor shifts were observed in bacterial family composition in the manure when refrigerated or frozen for 24 days, but significant population changes were observed when the manure was incubated for 24 days at 27?C. Since it is the temperature and incubation time that yielded the greatest pupation rate, it is hypothesized that the manure microbial community impacts the growth and development of stable flies. This study has determined suggested freezer conditions for the best storage of manure samples to maintain bacterial diversity and retain the closest bacterial populations to freshly collected manure. Although untouched, aged (20 days) manure is best to use to assess fly development, it is not always feasible in laboratory experimentations. This study demonstrates the importance of preservation techniques on manure samples, which could also confer to storage of other biological specimens that cont

关键词： stomoxys calcitrans L. Manure Microbiota 16S Metagenomics

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