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Quantitative trait loci identification reveals zinc finger protein CONSTANS-LIKE 4 as the key candidate gene of stigma color in watermelon(Citrullus lanatus)

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Journal of Integrative Agriculture 2024年第7期23卷 2292-2305页

作者： Shuang Pei Zexu Wu Ziqiao Ji Zheng Liu Zicheng Zhu Feishi Luan Shi Liu Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and RuralAffairs/College of Horticulture and Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030China Institute of Rural Revitalization Science and Technology Heilongjiang Academy of Agricultural SciencesHarbin 150028China

stigma color is a critical agronomic trait in watermelon that plays an important role in ***,there are few reports on the regulation of stigma color in *** this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow *** segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma ***,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated ***97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon *** expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing *** color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma *** data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma *** study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.

关键词： watermelon stigma color gene mapping zinc finger protein CONSTANS-LIKE 4

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QTL-seq identifies major quantitative trait loci of stigma color in melon

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Horticultural Plant Journal 2021年第4期7卷 318-326页

作者： Aohan Qiao Xufeng Fang Shi Liu Hongyu Liu Peng Gao Feishi Luan Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural AffairsNortheast Agricultural UniversityHarbin 150030China College of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030China

stigma color plays an important role in *** nature,melon(Cucumis melo L.)stigmas are either yellow or green;however,a review of the literature found no report on how stigma color affects pollination and fruit development in ***,we used an F_(2)melon population derived from a cross between‘MR-1’(P_(1),with green stigmas)and‘M1–32’(P_(2),with yellow stigmas),and performed genetic analysis and *** results of bulked segregant analysis allowed the identification of genetic loci controlling stigma color on chromosomes 6 and *** F2 population consisting of 150 individuals was used for initial mapping.A genetic map of 304.17 cM was constructed using 37 cleaved amplified polymorphism sequence(CAPS)*** identified one major quantitative trait locus(QTL)and one minor QTL for stigma *** major QTL GS8.1 was further mapped to a 4.13 cM interval between CAPS markers 8C-10 and 8C-16,which explained 27.04%of the phenotypic *** addition,GS6.1 was mapped between E-49 and 6A-7,explaining 18.6%of the phenotypic *** study provides a theoretical basis for the fine mapping and cloning of melon genes controlling stigma color.

关键词： Cucumis melo L. stigma color BSA QTL chlorophyll content CAPS

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