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Induced Neural stem cells Generated from Rat Fibroblasts

Genomics, Proteomics & Bioinformatics 2013年第5期11卷 312-319页

作者： Guangjun Xi Pingfang Hu Cunye Qu Shenfeng Qiu Chang Tong Qi-Long Ying Eli and Edythe Broad Center for Regenerative Medicine and stem cell Research at USC Department of Cell and NeurobiologyUniversity of Southern California Department of Neurology Wuxi People’s Hospital Affliated to Nanjing Medical University Department of Gastroenterology Changzheng HospitalSecond Military Medical University Zilkha Neurogenetic Institute University of Southern California

The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem （ES） cell cultures. Using a cocktail of cytokines and small molecules, we dem- onstrate that primitive neural stem （NS） cells derived from mouse ES cells and rat embryos can be maintained. Furthermore, using the same set of cytokines and small molecules, we show that induced NS （iNS） cells can be generated from rat fibroblasts by forced expression of the transcrip- tional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.

关键词： Induced neural stem cells stem cell self-renewal Fibroblasts Rat Induced pluripotent stemcells

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A single copy of large tumor suppressor 1 or large tumor suppressor 2 is sufficient for normal hematopoiesis

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Chinese Medical Journal 2020年第16期133卷 1943-1951页

作者： Zhi-Gang Li Xue-Mei Fu Cheng-Yan Chai Fang-Fang Sun Fei-Fei Xiao Yong-Xiu Huang Kai Yao Jie-Ping Chen Yu Hou Department of Hematology Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 430008China Medical Research Center Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 430008China College of Life Sciences and Health Wuhan University of Science and TechnologyWuhanHubei 430081China

Background:Hematopoietic stem cells(HSCs)have the ability to differentiate into all subsets of blood cells and *** tumor suppressor 1(LATS1)and large tumor suppressor 2(LATS2)kinases are essential for cell cycle regulation,organism fitness,genome integrity,and cancer ***,we investigated whether Lats1 and Lats2 are critical for the maintenance of the selfrenewal and quiescence capacities of HSCs in ***:Quantitative reverse transcription-polymerase chain reaction was used to determine the expression levels of Lats1 and Lats2 in subsets of progenitor cells and mature bone marrow cells.A clustered regularly interspaced short palindromic repeats system was used to generate Lats1 or Lats2 knockout *** blood cell counts were used to compare the absolute number of white blood cells,lymphocytes,monocytes,neutrophils,and platelets between Lats1 or Lats2 heterozygotes and *** cytometry was used to assess the size of hematopoietic progenitor cells(HPCs)and HSC pools in Lats1 or Lats2 heterozygotes and *** comparison between the two groups was analyzed using Student’s t ***:Lats1 and Lats2 were widely expressed in hematopoietic cells with higher expression levels in primitive hematopoietic cells than in mature ***1 or Lats2 knockout mice were generated,with the homozygotes showing embryonic *** size of the HPC and HSC pools in Lats1(HPC:wild-type[WT]***,220,426.77±54,384.796 vs.221,149.4±42,688.29,P=0.988;HSC:WT ***,2498.932±347.856 vs.3249.763±370.412,P=0.105)or Lats2(HPC:WT ***,425,540.52±99,721.86 vs.467,127.8±89,574.48,P=0.527;HSC:WT ***,4760.545±1518.01 vs.5327.437±873.297,P=0.502)heterozygotes were not ***,the depletion of Lats1 or Lats2 did not affect the overall survival of the heterozygotes(Lats1:P=0.654;Lats2:P=0.152).Conclusion:These results indicate that a single allele of Lats1 or Lats2 may be sufficient for nor

关键词： Hippo pathway Hematopoietic stem cells stem cell self-renewal

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