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Chart Review of PACU Outcomes for Patients Who Had Ambulatory Shoulder Surgery with Peripheral Nerve Block (PNB) and General Anesthesia Compared to General Anesthesia (GA)

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Open Journal of Anesthesiology 2015年第7期5卷 173-176页

作者： Dennis E. Feierman Eliezer Klinkowitz Charles Keilin Mark Kronenfeld David Rahmani Darlene Saberito Piyush Gupta Department of Anesthesiology Maimonides Medical Center Brooklyn NY USA

Introduction: Today, regional anesthetics are frequently used in combination with general anesthesia. The purpose of two different techniques is to attain distinct goals. We believed that the use of PNB with the minimization of GA in the ambulatory setting would result in a decreased opioid requirement and subsequently fewer treatment-related side effects, more awake and pain free patients and shorter PACU stays and earlier discharges when compared with general anesthesia alone. Methods: Prior to the start of this retrospective chart review, IRB approval was obtained. Patient’s charts were selected based on the following criteria: The control group had a general anesthetic (GA) and did not have a PNB, and the experimental group (PNB/GA) had a PNB and a general anesthetic. Our primary endpoints were PACU length of stay, pain scores, use of opioids and uses of anti-emetics. Results: We found that patient with blocks used less analgesic in the PACU, 3.97 mg vs. 1.39 mg (morphine equivalents). They also appeared to use less antiemetic drugs: 14/19 (patient s without PNB) vs. 7/18. Additionally, the patients that received a block had a statistically shorter PACU stay 107 min vs. 163 min. Conclusion: Patients that did not receive PNB had a significantly longer PACU stay. Additionally the data suggest that the use of PNB’s reduces the use of post-op narcotics, which may be related to the lower use of postoperative antiemetic drugs observed.

关键词： Ambulatory Nerve Block PACU Length of stay

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水稻SGR酵母双杂交诱饵载体的构建及鉴定

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安徽农业科学 2008年第17期36卷 7150-7151,7303页

作者：兰兆吉吴国江中国科学院华南植物园

[目的]揭示SGR基因的作用机理。[方法]构建水稻SGR基因在酵母双杂交体系中的诱饵载体,并对其表达进行鉴定,将目的基因SGR与诱饵质粒载体pGBKT7通过双酶切定向重组构建诱饵质粒pGBKT7-SGR,将pGBKT7-SGR转入酵母菌株Y187中,利用Western印... 详细信息

[目的]揭示SGR基因的作用机理。[方法]构建水稻SGR基因在酵母双杂交体系中的诱饵载体,并对其表达进行鉴定,将目的基因SGR与诱饵质粒载体pGBKT7通过双酶切定向重组构建诱饵质粒pGBKT7-SGR,将pGBKT7-SGR转入酵母菌株Y187中,利用Western印迹法检测其在酵母中的表达,通过缺陷性培养基培养进行自激活检测。[结果]将鉴定正确的重组质粒测序结果与SGR基因比较,序列完全一致,阅读框分析正确。载体pGBKT7-SGR在酵母菌株Y187中可以正确表达出融合蛋白。重组诱饵pGBKT7-SGR质粒对酵母无毒性,其表达产物不能激活酵母菌株Y187的营养缺陷型报告基因。[结论]该重组诱饵质粒可用于酵母双杂交体系,该研究为从cDNA文库筛选水稻诱饵蛋白SGR的互作蛋白奠定了基础。

关键词： stay green 酵母双杂交系统 westem印迹自激活检测

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