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TabHLH95-TaNF-YB1 module promotes grain starch synthesis in bread wheat

Journal of Genetics and Genomics 2023年第11期50卷 883-894页

作者： Yunchuan Liu Wei Xi Xiaolu Wang Huifang Li Hongxia Liu Tian Li Jian Hou Xu Liu Chenyang Hao Xueyong Zhang Key Laboratory of Crop Gene Resources and Germplasm Enhancement Ministry of Agriculture and Rural Affairs/The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Aridland Crop Science(Gansu Agricultural University)/Gansu Provincial Key Laboratory of Crop Improvement&Germplasm Enhancement LanzhouGansu 730070China College of Agronomy Gansu Agricultural UniversityLanzhouGansu 730070China

starch is the most abundant substance in wheat(Triticum aestivum L.)endosperm and provides the major carbohydrate energy for human daily *** synthesis-related(SSR)genes are believed to be spatiotemporally specific,but their transcriptional regulation remains unclear in ***,we investigate the role of the basic helix-loop-helix(bHLH)transcription factor TabHLH95 in starch ***95 is preferentially expressed in the developing grains in wheat and encodes a nucleus localized protein without autoactivation *** Tabhlh95 knockout mutants display smaller grain size and less starch content than wild type,whereas overexpression of TabHLH95 enhances starch accumulation and significantly improves thousand grain *** analysis reveals that the expression of multiple SSR genes is significantly reduced in the Tabhlh95 ***95 binds to the promoters of ADP-glucose pyrophosphorylase large subunit 1(AGPL1-1D/-1B),AGPL2-5D,and isoamylase(ISA1-7D)and enhances their ***,TabHLH95 interacts with the nuclear factor Y(NF-Y)family transcription factor TaNF-YB1,thereby synergistically regulating starch *** results suggest that the TabHLH95-TaNF-YB1 complex positively modulates starch synthesis and grain weight by regulating the expression of a subset of SSR genes,thus providing a good potential approach for genetic improvement of grain productivity in wheat.

关键词： starch synthesis TabHLH95-TaNF-YB1 module Synergistic regulation Grain weight Wheat

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Modulation of starch synthesis in Arabidopsis via phytochrome B-mediated light signal transduction

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Journal of Integrative Plant Biology 2024年第5期66卷 973-985页

作者： Qingbiao Shi Ying Xia Na Xue Qibin Wang Qing Tao Mingjing Li Di Xu Xiaofei Wang Fanying Kong Haisen Zhang Gang Li National Key Laboratory of Wheat Improvement College of Life SciencesShandong Agricultural UniversityTai'an 271018China National Key Laboratory of Wheat Improvement College of AgronomyShandong Agricultural UniversityTai'an 271018China

starch is a major storage carbohydrate in plants and is critical in crop yield and *** synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise molecular mechanisms governing this process remain largely *** this study,we revealed that high red to far-red(high R:FR)light significantly induces the synthesis of leaf starch and the expression of synthesis-related genes,whereas low R:FR light suppress these *** phytochrome B(phyB),the primary R and FR photoreceptor,was identified as a critical positive regulator in this *** of phyB,basic leucine zipper transcription factor ELONGATED HYPOCOTYL5(HY5)was found to enhance starch synthesis,whereas the basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORs(PIF3,PIF4,and PIF5)inhibit starch synthesis in Arabidopsis ***,HY5 and PIFs directly compete for binding to a shared G-box cis-element in the promoter region of genes encoding starch synthases GBSS,SS3,and SS4,which leads to antagonistic regulation of their expression and,consequently,starch *** findings highlight the vital role of phyB in enhancing starch synthesis by stabilizing HY5 and facilitating PIFs degradation under high R:FR light ***,under low R:FR light,PIFs predominantly inhibit starch *** study provides insight into the physiological and molecular functions of phyB and its downstream transcription factors HY5 and PIFs in starch synthesis regulation,shedding light on the regulatory mechanism by which plants synchronize dynamic light signals with metabolic cues to module starch synthesis.

关键词： Arabidopsis thaliana HY5 light signal phyB PIFs starch synthesis

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Effect of high temperature on the expressions of genes encoding starch synthesis enzymes in developing rice endosperms

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Journal of Integrative Agriculture 2015年第4期14卷 642-659页

作者： CAO Zhen-zhen PAN Gang WANG Fu-biao WEI Ke-su LI Zhao-wei SHI Chun-hai GENG Wei CHENG Fang-min College of Agriculture and Biotechnology Zhejiang University

High temperature is the major environmental factor affecting grain starch properties of cooking rice cultivars. In this study, two non-waxy indica rice genotypes, cv. 9311 and its mutant with extremely high amylose phenotype（9311eha） were used to study the differential expressions of genes in starch synthesis and their responses to high temperature（HT）. Significant increase in apparent amylose content and hot-water-soluble starch content in mutant 9311 eha were genetically caused by a substitution from AGTTATA to AGGTATA at the leader intron 5′ splice site in Wx gene. This mutation resulted in different m RNA transcript levels, m RNA splicing efficiencies and protein levels of Wx between the two rice genotypes, which also lead to the genotype-dependent alteration in the temporal pattern of Wx transcription and granule-bound starch synthase（GBSS） activity in response to HT. However, changes in the activities of other starch synthesizing enzymes and their expressions of distinct isoform genes were not significant with the Wx gene mutation, thus only minor difference in the particle size of starch granule, chain-length distribution and gelatinization enthalpy were found between the two genotypes. The temporal-specific expression of multiple isoform genes responsive to different temperature regiments indicated that the reduction of GBSS transcript expression under HT was generally accompanied by the decreased expressions of SSSIIa, SSSIIIa and SBEIIb. Consequently, high temperature-ripened grains in 9311 eha showed high proportion of intermediate and long B chains and somewhat lower level of short A chain compared to the wildtype. The temperature-dependent alteration of amylose content was not only attributed to the reduced expression of GBSS, but also associated with the complimentary effect of SSSIIa and SBEIIb.

关键词： rice starch synthesis gene expression amylopectin strucrute high temperature

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Transcription factor ZmNAC126 plays an important role in transcriptional regulation of maize starch synthesis-related genes

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The Crop Journal 2021年第1期9卷 192-203页

作者： Qianlin Xiao Yayun Wang Hui Li Chunxia Zhang Bin Wei Yongbin Wang Huanhuan Huang Yangping Li Guowu Yu Hanmei Liu Junjie Zhang Yinghong Liu Yufeng Hu Yubi Huang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Agronomy and Biotechnology Southwest UniversityChongqing 400716China College of Agronomy Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Life Science Sichuan Agricultural UniversityYa'an 625014SichuanChina Maize Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Sichuan Seed Station Chengdu 610041SichuanChina

Maize(Zea mays L.)is one of the most important food crops in the world,and starch is the main component of its *** regulation plays a vital role in starch ***,it is not well understood in *** report the identification of the transcription factor ZmNAC126 and its role in regulation of starch synthesis in *** expression of ZmNAC126 was higher in maize endosperm and kernels than in roots or ***126 shared a similar expression pattern with starch synthesis genes during seed development,and its expression pattern was also consistent with the accumulation of ***126 is a typical transcription factor with a transactivation domain between positions 201 and 227 of the amino acid sequence,is located in the nucleus,and binds to CACG repeats in *** one-hybrid assay revealed that ZmNAC126 bound the promoters of ZmGBSSI,ZmSSIIa,ZmSSIV,ZmISA1,and *** overexpression of ZmNAC126 in maize endosperm increased the activities of promoters pZmSh2,pZmBt2,pZmGBSSI,pZmSSIIIa,and pZmBT1 but inhibited the activities of pZmISA1 and ***126 thus acts in starch synthesis by transcriptionally regulating targeted starch synthesis-related genes in maize kernels.

关键词： Maize starch synthesis ZmNAC126 Co-expression Transcription regulation

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Moderate drought alleviates the damage to grain quality at high temperatures by improving the starch synthesis of inferior grains in japonica rice

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Journal of Integrative Agriculture 2022年第10期21卷 3094-3101页

作者： TONG Hui DUAN Hua WANG Sheng-jun SU Jing-ping SUN Yue LIU Yan-qing TANG Liang LIU Xue-jun CHEN Wen-fu Rice Research Institute Shenyang Agricultural UniversityShenyang 110866P.R.China Tianjin Key Laboratory of Crop Genetics and Breeding Tianjin Crop Research InstituteTianjin 300384P.R.China

In agricultural production,temperature and moisture are important factors affecting grain yield and *** moderate drought at the grain-filling stage can effectively alleviate the damage caused by high temperature,the specific regulatory mechanism driving the effect of moderate drought at the high temperature on starch synthesis is still *** explore the effects and mechanisms of high temperature and moderate drought on rice starch synthesis at the grainfilling stage,the activities of enzymes and expression levels of the genes involved in starch synthesis under four different treatments involving high temperature and/or water stress(CK,HT,WS,and HT+WS)were investigated in this *** starch synthesis of a japonica inbred rice was measured under the four treatments during the grain *** results show that the effects of high temperature and moderate drought on grain filling mainly occur in the inferior grains of *** the regulation of enzymes involved in starch synthesis and the expression levels of their main genes,the synthesis of rice starch can be ***,the high temperature and moderate drought were antagonistic,and moderate drought can alleviate the damage to grain quality at a high temperature by improving the starch synthesis of inferior grains in japonica *** study provides a basis for stress-resistance cultivation and breeding strategies of rice with high temperature tolerance.

关键词： high temperature moderate drought antagonism starch synthesis japonica rice

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Molecular cloning,characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm,Lilium brownii ***

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第6期22卷 476-491页

作者： Yun WU Minyi SUN Shiqi LI Ruihan MIN Cong GAO Qundan LYU Ziming REN Yiping XIA Department of Landscape Architecture School of Civil Engineering and ArchitectureZhejiang Sci-Tech UniversityHangzhou 310018China Genomics and Genetic Engineering Laboratory of Ornamental Plants College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Chemical Biology Center Lishui Institute of Agriculture and Forestry SciencesLishui 323000China

starch is the predominant compound in bulb scales,and previous studies have shown that bulblet development is closely associated with starch ***,how starch synthesis affects bulbification at the molecular level is *** this study,we demonstrate that Lilium brownii ***,a wild lily with a giant bulb in nature,and ***,the native species,have different starch levels and characteristics according to cytological and ultra-structural *** cloned the complete sequence of three key gene-encoding enzymes(LbgAGPS,LbgGBSS,and LbgSSⅢ)during starch synthesis by rapid amplification of 5’and 3’complementary DNA(cDNA)ends(RACE)*** analysis revealed that the proteins deduced by these genes contain the canonical conserved *** phylogenetic trees confirmed the evolutionary relationships with proteins from other species,including monocotyledons and *** transcript levels of various tissues and time course samples obtained during bulblet development uncovered relatively high expression levels in bulblets and gradual increase expression accompanying bulblet ***,a set of single nucleotide polymorphisms(SNPs)was discovered in the AGPS genes of four lily genotypes,and a purifying selection fashion was predicted according to the non-synonymous/synonymous(Ka/Ks)*** together,our results suggested that key starch-synthesizing genes might play important roles in bulblet development and lead to distinctive phenotypes in bulblet size.

关键词： Lilium starch synthesis Bulblet development Single nucleotide polymorphism(SNP) Expression pattern

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Diurnal Changes in the Transcription Profiles of Genes Encoding starch synthesis-related Enzymes in Wheat Grains under Field Conditions

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Agricultural Science & Technology 2017年第4期18卷 565-569,574页

作者： Lina WANG Qiaoxia HAN Xianghong XIAO Tiancai GUO Guozhang KANG National Key Laboratory of Wheat and Maize Crop Science Henan Agricultural University Zhengzhou 450002 China Collaborative Innovation Center of Henan Food Crops Henan Agricultural University Zhengzhou 450002 China National Engineering Research Centre for Wheat Henan Agricultural University Zhengzhou 450002 China

[Objective] During the filling stage of plant growth and development, storage starch is diurnally synthesized and accumulated in the grains from cereal crops, but the underlying molecular mechanism is unclear. [Method] In this study, grains from the bread wheat cultivar Zhoumai 18 grown in fields were harvested at 15 d after anthesis, and quantitative real-time reverse transcription polymerase chain reaction(qPCR) was used to measure the transcriptional levels of 26 genes encoding starch synthesis-related enzymes at 2 h intervals throughout a diurnal cycle. [Result] Our findings indicated that storage starch was persistently synthesized in wheat grains throughout a 24 h period. The diurnal patterns of the transcriptional levels of 26 genes in wheat grains were classified into two groups. The 13 genes in Group 1 were temporally and highly expressed in wheat grains,and their encoded proteins could play crucial roles in starch synthesis. The other 13 genes in Group 2 were characterized by low or no transcription in wheat grains throughout a diurnal cycle, suggesting their function in the synthesis or degradation of transitory starches in wheat grains. [Conclusion] These results provide information on the molecular mechanism of storage starch synthesis in higher plants.

关键词： Diurnal change Gene expression starch synthesis Transcriptional level Triticum aestivum L.

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Expression Regulation of Seed Storage Proteinand starch synthesis in Common Wheat

Expression Regulation of Seed Storage Proteinand Starch Synt...

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第八届全国小麦基因组学及分子育种大会

作者： Lisha Shen Guangbin Luo Yanhong Song Shuyi Song Wenlong Yang Jiazhu Sun Kehui Zhan Aimin Zhang Dongcheng Liu State Key Laboratory of Plant Cell and Chromosome Engineering National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyChinese Academy of sciences College of Agronomy The Collaborative Innovation Center of Grain Crops in HenanHenan Agricultural University

Seed storage proteins(SSPs) and starch are main components in wheat grain,and their contents and compositions determine the flour processing quality and yield,*** expression levels of SSP genes and starch synthesis related genes(SSRGs) are positively correlated with the final accumulation of SSPs and ***,the process of SSP and starch synthesis was poorly understood,and only a few transcription factors(TF,***,R2 R3 MYB and Dof) have been *** this work,the transcriptome profiling of maturing endosperms[5,10,15,20 and 25 days post anthesis(DPAs)]in Chinese Spring were investigated though both RNA-Seq and miRNA-Seq *** the grain-filling stages,the expression of SSP genes initiated at 5 DP A,peaked at 20 DP A,and then declined *** shared similar expression model,which started at 5 DP A,and reached at their peaks at 10 DP A and then decreased,but the accumulation of amylose and amylopectin had different patterns along with the grain filling *** the RNA-Seq assay,74 TFs were observed co-expressed with SSP genes and SSRGs,while 61 miRNAs had similar expression pattern with SSP genes and SSRGs according to the miRNA-Seq *** TFs and miRNAs might regulate the SSP gene and SSRG expression due to their co-existence in the filling wheat ***,except these previously reported bZIP,R2 R3 MYB and Dof,much more gene families might involve in the SSP and starch synthesis,***,AIL5,ESR1,MADS,DRFL,GL9 H2,KAN2 and WRKY,though a detailed work on these genes was necessary in the ***,nine novel miRNAs were also *** data would provide the fundamental information for us to understand the SSP and starch synthesis in common wheat.

关键词： Seed storage protein starch synthesis Transcription fector miRNA Expression regulation

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FGW1, a protein containing DUF630 and DUF632 domains, regulates grain size and filling in Oryza sativa L.

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The Crop Journal 2023年第5期11卷 1390-1400页

作者： Yangyang Li Peilong He Xiaowen Wang Hongyan Chen Jile Ni Weijiang Tian Xiaobo Zhang Zhibo Cui Guanghua He Xianchun Sang Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China Guizhou University of Traditional Chinese Medicine School of Pharmacy Guiyang 550025GuizhouChina

Grain filling influences grain size and quality in cereal crops. The molecular mechanisms that regulate grain endosperm development remain elusive. In this study, we characterized a filling-defective and grain width mutant, fgw1, whose mutation increased rice seed width mainly via cell division and expansion in grains. Sucrose contents were higher but starch contents lower in the fgw1 mutant during the grainfilling stage, resulting in inferior endosperm of opaque, white appearance with loosely packed starch granules. Map-based cloning revealed that FGW1 encoded a protein containing DUF630/DUF632domains, localized in the plasma membrane with preferential expression in the panicle. RNA interference in FGW1 resulted in increased grain width and weight, whereas overexpression of FGW1 led to slightly narrower kernels and better grain filling. In a yeast two-hybrid assay, FGW1 interacted directly with the 14–3–3 protein GF14f, bimolecular fluorescence complementation verified that the site of interaction was the membrane, and the mutated FGW1 protein failed to interact with GF14f. The expression of GF14f was down-regulated in fgw1, and the activities of AGPase, StSase, and SuSase in the endosperm of fgw1increased similarly to those of a reported GF14f-RNAi. Transcriptome analysis indicated that FGW1 also regulates cellular processes and carbohydrate metabolism. Thus, FGW1 regulated grain formation via the GF14f pathway.

关键词： Seed size Grain filling DUF630/DUF632 starch synthesis Rice

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Cloning and characterization of a glucose 6-phosphate／phosphate translocator from Oryza sativa

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Journal of Zhejiang University Science 2003年第3期4卷 331-335页

作者：姜华武佃蔚敏刘非燕吴平 TheStateKeyLaboratoryofPlantPhysiologyandBiochemistry CollegeofLifesciencesZhejiangUniversityHangzhou310029China

Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6 phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6 phosphate/phosphate translocator ( GPT ) was isolated from a cDNA library of immature seeds of rice and named as OsGPT . The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000 grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6 phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids.

关键词： Glucose 6 phosphate/phosphate translocator starch synthesis Rice ( Oryza sativa L.)

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