检索结果-南通市图书馆

Molecular study on methicillin-resistant staphylococcus aureus strains isolated from dogs and associated personnel in Jordan

引用

Asian Pacific Journal of Tropical Biomedicine 2015年第11期5卷 902-908页

作者： Yaser Hamadeh Tarazi Ahmed Mahmoud Almajali Mustafa Mohammad Kheer Ababneh Humam Shawket Ahmed Adnan Saleem Jaran Department of Basic Veterinary Medical Sciences Faculty of Veterinary MedicineJordan University of Science and Technology Department of Biological Sciences Faculty of ScienceAl Al-Bayt University

Objective: To determine the prevalence, genetic relatedness, and pattern of antimicrobial susceptibility in methicillin-resistant staphylococcus aureus(S. aureus)(MRSA) isolated from household dogs, farm dogs, and stray dogs, compared to isolates from their associated ***: MRSA was isolated from 250 nasal swabs(150 swabs from dogs and 100 swabs from humans). PCR assays were used to detect the presence of both the nuc and mec A genes,which con firmed the identity of S. aureus isolates and the presence of methicillin resistance,respectively. Disk diffusion was used to determine the antibiotic susceptibility against 15 antimicrobial agents along with an E-test that determined the minimum inhibitory concentration for oxacillin. Pulsed field gel electrophoresis was conducted to determine the genetic relatedness of MRSA isolates from dogs to those from associated and unassociated ***: The prevalence of S. aureus in dogs and humans was 12.7% and 10.0%respectively, while the prevalence of MRSA isolates in dogs and humans was 5.3% and5.0%, respectively. The prevalence of MRSA isolates in household dogs, farm dogs, and stray dogs was 7.8%, 4.7%, and 0.0%, respectively. MRSA isolates demonstrated a significantly higher rate of multi-resistance against three or more antimicrobial agents than methicillin-susceptible S. aureus(MSSA). Trimethoprim-sulphamethoxazole and chloramphenicol were the most effective antibiotics against all MRSA isolates. Pulsed field gel electrophoresis revealed a strong association between dog MRSA isolates and MRSA isolates from strongly associated ***: MRSA is prevalent in house dogs, as well as in dog rearing centers and among their strongly associated personnel. A strong association was found between the MRSA isolates from dogs and those from humans who are in close contact. In addition,MRSA isolates showed a high rate of multi-resistance compared to MSSA isolates.

关键词： staphylococcus aureus Methicillin-resistant staphy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparative study on production,purification of penicillin by Penicillium chrysogenum isolated from soil and citrus samples

引用

Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 15-19页

作者： S Anto Jeya Dayalan Pramod Darwin Prakash S Department of Biotechnology Udaya School of Engineering

Objective:To explore various unexplored locations where Penicillium *** be available and study the production of penicillin from the isolated Penicillium *** different media with altered carbohydrate ***:The collected soil samples were screened for the isolation of Penicillium chrysogenum(***) by soil dilution *** isolated Penicillium species were further grown in different production media with changes in the carbohydrate *** extracted penicillin from various isolates was analyzed by HPLC for the efficacy of the *** the products were screened with various bacterial species including methicillin resistant staphylococcus aureus(MRSA).And the work was extended to find the possible action on MRSA,along with characterization using other ***:From the various soil and citrus samples used for analysis,only the soil sample from Government General Hospital of Bangalore,India,and Sanjay Gandhi Hospital,Bangalore,India,showed some potential growth of the desired fungi *** production media showed varied range of growth of *** production of penicillin was obtained in maltose which proved maximum zone of inhibition during *** of penicillin on pathogens,like wild Escherichia coli strain,Klebsiella spp.,and MRSA,gave quite interesting results such as no activity on the later strain as it is *** data provided the analytical and confirmation details of the penicillin ***,the penicillin produced from the soil sample of Government General Hospital had the high milli absorbance unit of 441.5 mAu compared with that of the penicillin produced from Sanjay Gandhi Hospital sample,8S.S2 ***,there was a considerable change in quantity of the penicillin produced from both the ***: The Penicillium *** be possibly rich in hospital contaminants and its *** research focuses on various unexplored sources o

关键词： Penicillium chrysogenum Soil Citrus Penicillin Bioefficacy Antibiotic activity Penicillium spp. Production Soil dilution plate staphy lococcus aureus Characterization Production media Zone of inhibition Carbohydrate source Isolation Efficacy Pathogen

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：