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A revisit to staining reagents for neuronal tissues

Annals of Eye Science 2022年第1期7卷 51-65页

作者： Alexandra Rosario Ashley Howell Sanjoy K.Bhattacharya Miami Integrative Metabolomics Research Center Bascom Palmer Eye InstituteUniversity of MiamiMiamiFloridaUSA

In the early days of deciphering the injured neuronal tissues led to the realization that contrast is necessary to discern the parts of the recovering tissues from the damaged *** attempts relied on available(and often naturally occurring)staining ***,the active ingredients of most of them were small *** the advent of time,the knowledge of chemistry helped identify compounds and conditions for *** staining reagents were even found to enhance the visibility of the *** impregnation identification of Golgi bodies was discovered in owl optic *** reagents since the late 1800s were widely used across all disciplines and for nerve tissue and became a key contributor to advancement in nerve-related *** use of these reagents provided insight into the organization of the neuronal tissues and helped distinguish nerve degeneration from *** neuronal staining reagents have played a fundamental role in the clinical research facilitating the identification of biological mechanisms underlying eye and neuropsychiatric *** found a lack of systematic description of all staining reagents,whether they had been used historically or currently *** is a lack of readily available information for optimal staining of different neuronal tissues for a given *** present here a grouping of the reagents based on their target location:(I)the central nervous system(CNS),(II)the peripheral nervous system(PNS),or(III)*** biochemical reactions of most of the staining reagents is based on acidic or basic pH and specific reaction partners such as organelle or biomolecules that exists within the given tissue *** present here a summary of the chemical composition,optimal staining condition,use for given neuronal tissue and,where possible,historic *** biomolecules such as lipids and metabolites lack specific *** being non-specific the reagents enhance contrast a

关键词： staining reagent neuroregeneration silver impregnation method Mallory staining hematoxylin-eosin staining

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