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spy chemistry enables stable protein immobilization on iron oxide nanoparticles with enhanced magnetic properties

Journal of Materials Science & Technology 2023年第30期161卷 161-169页

作者： Chu Shi Jingyi Sheng Guangxiang Si Yan Li Yan Shen Ning Gu Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210009China School of Biomedical Engineering and Informatics Nanjing Medical UniversityNanjing 211166China Medical School Nanjing UniversityNanjing 210093China

Iron oxide nanoparticles(IONPs)modified with functional proteins hold great promise in the biomedical ***,conventional protein modification strategies,such as adsorption and covalent coupling,are either unstable or nonspecific,or may result in the changes of protein structure and ultimately the loss of protein *** of active proteins on small-sized IONPs with a particle size of less than 30 nm is especially difficult due to their high surface ***,we developed a universal modifica-tion method based on spy chemistry for rapid and stable protein immobilization on small-sized IONPs,which only requires the presence of active groups on the surface of nanoparticles that can couple with *** short,the spyCatcher peptides were first coated on the surface of IONPs by cross-linking with activated groups,and then the spyTag peptide fused with a model protein(enhanced green fluo-rescent protein,EGFP)was engineered(spyTag-EGFP)and directly coupled to spyCatcher-modified IONPs by self-assembly,which is spontaneous and robust while avoiding the effect of chemical reactions on functional protein *** obtained EGFP-functionalized IONPs exhibited enhanced and stable green fluorescence and improved magnetic *** addition,the cell internalization efficiency of EGFP-functionalized IONPs was significantly increased as compared to unmodified IONPs,providing an ideal solution for efficient cell labeling and *** conclusion,here we report a rapid and easy strategy for EGFP immobilization on IONPs based on spy chemistry,which could be further adapted to other functional proteins in the ***-modified IONPs and spyTag-X(arbitrary functional fusion proteins)hold great potential to be applied as a versatile platform for protein immobilization on IONPs and enable its multifunctional application in the future.

关键词： Iron oxide nanoparticles spy chemistry Protein immobilization Magnetic resonance imaging Cell labeling

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