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Detection of spores using polarization image features and BP neural network

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International Journal of Agricultural and Biological Engineering 2024年第5期17卷 213-221页

作者： Yafei Wang Ning Yang Guoxin Ma Mohamed Farag Taha Hanping Mao Xiaodong Zhang Qiang Shi School of Agricultural Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Jiangsu Changdian Technology Co. Ltd.Jiangyin 214400JiangsuChina School of Electrical and Information Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Department of Soil and Water Sciences Faculty of Environmental Agricultural SciencesArish UniversityNorth Sinai 45516Egypt School of Science and Technology Shanghai Open UniversityShanghai 200433China

Timely detection and control of airborne disease is important to improve productivity. This study proposed a novel approach that utilizes micro polarization image features and a backpropagation neural network (BPNN) to classify and identify airborne disease spores in a greenhouse setting. Firstly, disease spores were collected in the greenhouse, and their surface morphological parameters were analyzed. Subsequently, the micropolarization imaging system for disease spores was established, and the micropolarization images of airborne disease spores from greenhouse crops were collected. Then the micropolarization images of airborne disease spores were processed, and the image features of polarization degree and polarization angle of disease spores were extracted. Finally, a disease spore classification model based on the BPNN was ultimately developed. The results showed that the texture position of the surface of the three disease spores was inconsistent, and the texture also showed an irregular shape. Texture information was present on the longitudinal and transverse axes, with the longitudinal axis exhibiting more uneven texture information. The polarization-degree images of the three disease spores exhibit variations in their representation within the entirety of the beam information. The disease spore polarization angle image exhibited the maximum levels of contrast and entropy when the Gabor filter’s direction was set to π/15. The recognition accuracy of cucumber downy mildew spores, tomato gray mildew spores, and cucumber powdery mildew spores were 75.00%, 83.33%, and 96.67%, respectively. The average recognition accuracy of disease spores was 86.67% based on BPNN and micropolarization image features. This study can provide a novel method for the detection of plant disease spores in the greenhouse.

关键词： greenhouse spores micropolarization image BPNN image processing detection

Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials

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Synthetic and Systems Biotechnology 2021年第3期6卷 180-191页

作者： Muhammad Zubair Mohsin Rabia Omer Jiaofang Huang Ali Mohsin Meijin Guo Jiangchao Qian Yingping Zhuang State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237PR China

Bacillus subtilis is a commonly used commercial specie with broad applications in the fields of bioengineering and *** is capable of producing both biofilms and *** are matrix-encased multicellular communities that comprise various components including exopolysaccharides,proteins,extracellular DNA,and poly-γ-glutamic *** biofilms resist environmental conditions such as oxidative stress and hence have applications in bioremediation ***,biofilms and spores can be engineered through biotechnological techniques for environmentally-friendly and safe production of bio-products such as *** ability to withstand with harsh conditions and producing spores makes Bacillus a suitable candidate for surface display *** recent years,the spores of such specie are widely used as it is generally regarded as safe to *** in synthetic biology have enabled the reprogramming of biofilms to improve their functions and enhance the production of value-added ***,there is increased interest in the production of engineered biosensors,biocatalysts,and *** elastic modulus and gel properties of *** biofilms have been utilized to develop living *** review outlines the formation of *** biofilms and *** engineering processes and their increasing application in bioremediation and biocatalysis,as well as the future directions of *** biofilm engineering,are ***,the ability of *** biofilms and spores to fabricate functional living materials with self-regenerating,self-regulating and environmentally responsive characteristics has been *** review aims to resume advances in biological engineering of *** biofilms and spores and their applications.

关键词： Bacillus subtilis Biofilms spores Biocatalysis Bioremediation Biomaterials Synthetic biology

Fertile organs and in situ spores of a matoniaceous fern from the Lower Jurassic of West Hubei

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Chinese Science Bulletin 1999年第14期44卷 1333-1337页

作者： Yongdong Wang Shengwu Mei Acad Sinica Nanjing Inst Geol & Palaeontol Nanjing 210008 Peoples R China

Fossil matoniaceous plants represented by Phlebopteris have been formerly descrinbea and illustrated from the Mesozoic of China mainly based on vegetative leaves, and no information is provided on the fine structure of fertile organs. Recent reinvestigation of the Hsiangchi Flora from the Lower Jurassic of West Hubei, China, obtained a rich collection of well preserved Phlebopteris specimens (both sterile and fertile). Studies on Phlebopteris polypodioides Brongniart using LM and SEM reveal not only the structures of sori and sporangia, but also the details of in situ spores. Comparisons have been made between the in situ spores and other related fossil and extant matoniaceous spores, as well as the dispersed genera.

关键词： Phlebopteris Matoniaceae in situ spores Lower Jurassic Hubei.

ALGAL, spores AND POLLEN ASSEMBLAGES FROM LATE PLEISTOCENE IN THE EAST PART OF THE CHINA SEA AND THEIR PALEOENVIRONMENTS

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Science China Chemistry 1989年第7期32卷 850-858页

作者：王开发张玉兰蒋辉 Department of Marine Geology Tongji University Shanghai

The paper deals with fossil spores, pollen and algae from the sediments of 70,00-12,000a B. P. in the eastern sea area of China, distinguishing three spores, pollen and algal zones. Zone Ⅰ indicates that the climate was cold, the sea level was lower, the continental shelf of theDonghai Sea (the East China Sea)was exposed as the plain with various grasses thickened and manylakes andswamps dotted in which lived abundant freshwater algae of Pediastrum, Zygnema, *** hills in the continental margin were covered by the dark coniferous forests of Abies, Piceaand Pinus. The climate of Zone Ⅱ was warm, resulting in the sea level rising and the con-tinental shelf submerged as neritic environment with a variety of diatoms, dinoflagllate andDictyocha. The vegetation of the hills in the continental margin became mixed forests ofconiferous and broad-leaf trees mainly composed of Pinus and Quercus. Zone Ⅲ indicatesthat the climate became cold again and the continental shelf was exposed as plain with thesame landscape as that of Zone Ⅰ. The Huanghe River and the Changjiang River at that timeconverged and flowed across the plain and down to the Okinawa Trough.

关键词： algae spores pollen paleoenvironment

Influence of Long Chain Free Fatty Acids on the Thermal Resistance Reduction of Bacterial spores

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Food and Nutrition Sciences 2013年第9期4卷 150-157页

作者： Brice Mvou Lekogo Louis Coroller Pierre Mafart Ivan Leguerinel Laboratoire Universitaire de Biodiversité et Ecologie Microbienne Université de Brest UEB Quimper France.

Aims: The objective of this study was to investigate the effect of free fatty acid length chain and unsaturated bond number on the heat resistance and recovery media of bacterial spores. Methods and results: For 6 species, bacterial spore heat resistances were estimated at different free fatty acid concentrations added to heating media or in recovery media. The addition of free fatty acids to heating media has a slight influence on the heat resistance of bacterial spores whatever the species or type of acid studied. On the contrary, the addition of free fatty acids to the recovery medium after the heat treatment greatly reduces the ability to bacterial spores to recover and form colonies. This effect varies depending on chain length, unsaturated bond number of fatty acid and on the bacterial strain studied. Conclusion: The presence of free acids in the recovery media is an additive stress which decreases the capability of injured spores to germinate and grow thereafter. Significance and impact of this study: The impact of free fatty acids presented in this study can be taken into account to reduce the thermal intensity of food sterilization in relation to their availability in food matrix.

关键词： Free Fatty Acid Bacterial spores Heat Resistance

Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores

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Surface display of OmpC of Salmonella serovar Pullorum on Ba...

Palynofloral changes in the Upper Paleozoic and Mesozoic of the Deocha-Pachami area, Birbhum Coalfield, West Bengal, India

中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Xixi Dai Minggang Liu Kangcheng Pan Jinlong Yang Chongqing Academy of Animal Science College of Veterinary Medicine Sichuan Agricultural University

Salmonellosis is a major public health problem throughout the ***,there is a huge need for diversified control strategies for Salmonella *** this work,we have assessed the potential use of Bacillus subtilis(***) spores for the expression of a major protective antigen of Salmonella serovar Pullorum,*** expression of OmpC on the surface of spores was determined by immunofluorescence *** immunized with recombinant spores expressing the OmpC antigen presented significant levels of OmpC-specific serum IgG and mucosal SIgA antibodies than in mice immunized with non-recombinant spores(pspores was able to induced a significant level of protection in mice against lethal challenge with Salmonella serovar *** results suggest that *** spores have promising potential in the development of mucosal vaccines against Salmonella infections.

关键词： Bacillus subtilis spores Recombinant Surface display Salmonella pullorum OmpC Mucosal vaccines

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Science China Earth Sciences 2009年第12期52卷 1932-1952页

作者： VIJAYA Birbal Sahni Institute of Palaeobotany 53 University Road Lucknow 226007 India

The study of Upper Paleozoic and Mesozoic palynomorphs in three boreholes from the Deocha-Pachami area, Birbhum Coalfield, West Bengal, India, has allowed dating of the Talchir, Barakar, Dubrajpur, and Rajmahal formations, and revealed many hiatuses. The lowermost unit, the Talchir Formation, yielded earliest Permian palynomorphs. The Barakar Formation, which includes coal-bearing strata, was previously dated as Early Permian. However, data presented herein indicate an Early Permian to earliest Triassic age for this unit,containing actually the Karharbari, Barakar s.s., Kulti, and Ranigang formations as well as the basal part of the Panchet Formation. The overlying Dubrajpur Formation is Jurassic (Callovian to Tithonian), with an unconformity at its base. The uppermost Dubrajpur Formation is Tithonian-Berriasian. The palynomorphs from the intertrappeans within the Rajmahal Formation suggest an Early Cretaceous age. The revised ages of the Barakar and Dubrajpur formations are of major regional significance. The distribution patterns of spore-pollen may provide a broad spectrum of paleoclimate during Permian, Late Jurassic, and Early Cretaceous times, as there is no record of marine signatures in the study area.

关键词： Talchir to Rajmahal formations Upper Paleozoic Mesozoic spores and pollen biostratigraphy India

A New Species of Coniopteris moguqiensis sp. nov. from the Middle Jurassic Wanbao Formation in Eastern Inner Mongolia, China

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Acta Geologica Sinica(English Edition) 2019年第5期93卷 1317-1324页

作者： ZHANG Yujin LIU Bingcai LIANG Fei Shenyang Center of Geological Survey China Geological SurveyShenyang 110034China College of Paleontology Shenyang Normal UniversityShenyang 110034China Key Lab of Evolution of Past Life in NE Asia Ministry of Natural ResourcesShenyang 110034China State Key Laboratory of Palaeobiology and Stratigraphy(Nanjing Institute of Geology and Palaeontology CAS)Nanjing 210008China

In recent years, an increasing number of plant fossil leaves and petrified woods have been discovered from the Middle Jurassic Wanbao Formation in Moguqi Town of Inner Mongolia, NE China. Here, we describe a new species of Coniopteris moguqiensis sp. nov. preserved as a fragment with fertile and sterile pinnules. The sterile ultimate pinnules are elongate ovate with sphenopteriod type venation, and fertile pinnules are usually isolated, bipinnate at least with the sorus apical, elliptical, 1 mm in diameter;sporangia are almost globular, 100-150 μm in diameter, and the annulus is vertical. In situ spores are rounded-triangular in polar view, 25-30 μm in diameter with sides straight and slightly convex;trilete, laesurae are thin and slightly straight;the exine surface is usually psilate under the light microscope but finely reticuloid sculptured on the proximal view under a scanning electronic microscope. The fern genus Coniopteris usually suggests a warm and humid environment, which is consistent with the palaeoclimatic conditions of petrified wood and megafossil plants. The new discovery further supplements the floral composition of the Wanbao Formation, providing new material for understanding the evolutionary trend and classification of Coniopteris.

关键词： Coniopteris Wanbao Formation in situ spores Middle Jurassic Inner Mongolia

A high-resolution history of vegetation and climate history on Sunda Shelf since the last glaciation

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Science China Earth Sciences 2007年第1期50卷 75-80页

作者： Karl STATTEGGER Institute of Geosciences University of Kiel Olshausenstrasse 40 Kiel 24118 Germany

This paper presents 16500-year-long high-resolution pollen and spore records from sediments of core 18287 on the continental slope of the southern South China Sea. In the period of 16.5―13.9 ka B.P., the low-mountain rainforest dominated the continental slope of the South China Sea. And in the period of 13.9―10.2 ka B.P., the lowland rainforest and ferns expanded greatly, while the low-mountain rainforest shrank, which indicated a warming at the last deglaciation. Also during this period, the pollen sedi-mentation rates reduced sufficiently. This might imply a rise of the sea level and therefore the sub-mergence of the shelf, resulting in the broadening of the distance between the source area and the slope. After 10.2 ka B.P, decreasing of the fern indicates the early Holocene (10.2―7 ka B.P.) is a cold period, while the increasing of fern marks the rising temperature (7―3.6 ka B.P.).

关键词： Sunda Slope pollen and spores vegetation last deglaciation Holocene.