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Formation of microchimerism in rat small bowel transplantation by spienocyte infusion

World Journal of Gastroenterology 2006年第26期12卷 4166-4169页

作者： Da-Xun Piao Tao Jiang Lian-Xin Liu An-Long Zhu Shao-Feng Jin Ying-Hui Guan Department of General Surgery the First Affiliated Hospital of Harbin Medical University Harbin 150001 Heilongjiang Province China Department of General Surgery the Affiliated Hospital of Harbin Institute of Technology Harbin 150001 Heilongjiang Province China Department of General Surgery the Fourth Affiliated Hospital of Harbin Medical University Harbin 150001 Heilongjiang Province China

AIM： To investigate the effect of donor splenocyte infusion combined with cyclosporine A （CsA） on rejection of rat small bowel transplantation （SBT）. METHODS： Male Sprague-Dawley （SD） rats and female Wistar rats weighing 230-270 g were used as donors and recipients respectively in the study. Heterotopic small bowel transplantation was performed. The rats were divided into three groups： group one receiving allotransplantation （SD→Wistar）, group two receiving allotransplantation （SD→Wistar）＋ donor splenocyte infusion, group three receiving allotransplantation （SD →Wistar）＋ donor splenocyte infusion ＋ CsA followed by CsA 10 mg/kg per day after transplantation, in which recipient Wistar rats were injected with 2 ×10^8 SD splenocytes 28 d before transplantation, and treated with CsA after transplantation. Finally, the specific DNA fragment of donor Y chromosome was detected in recipient peripheral blood and skin by PCR. The survival time after small bowel transplantation was observed. Gross and histopathological examinations were performed. RESULTS： The survival time after small bowel transplantation was 7.1 ± 1.2 d in group 1, 18.4 ± 3.6 d in group 2 and 31.5± 3.1 d in group 3. The survival time was significant longer （P 〈 0.01） in group 3 than in groups 1 and 2. The gross and histopathological examination showed that the rejection degree in group 3 was lower than that in groups 1 and ***： Donor splenocyte infusion combined with CsA decreases remarkably the rejection and prolongs the survival time after rat small bowel transplantation.

关键词： Microchimerism splenocyte infusion Immunologic tolerance Small bowel transplantation

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