检索结果-南通市图书馆

spinal cord injury regeneration using autologous bone marrowderived neurocytes and rat embryonic stem cells:A comparative study in rats

在线全文

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2020年第12期12卷 1591-1602页

作者： Mir Sadat-Ali Dakheel A Al-Dakheel Ayesha Ahmed Haifa A Al-Turki Abdallah S Al-Omran Sadananda Acharya Methal I Al-Bayat Department of Orthopedic Surgery Imam Abdulrahman Bin Faisal University and King Fahd Hospital of the UniversityDammam 31952Saudi Arabia Department of Orthopedic Surgery Imam Abdulrahman Bin Faisal UniversityAlKhobar 31952Saudi Arabia Department of Pathology Imam Abdulrahman Bin Faisal UniversityDammam 31952Saudi Arabia Department of Obstetrics and Gynecology Imam Abdulrahman Bin Faisal UniversityDammam 31142Saudi Arabia Department of Orthopedic Surgery Imam Abdulrahman Bin Faisal University and King Fahd Hospital of the UniversityAlKhobar 31952Saudi Arabia Department of Public Health College of Public HealthImam Abdulrahman Bin Faisal UniversityDammam 31441Saudi Arabia

BACKGROUND spinal cord injury(SCI)is an important cause of traumatic paralysis and is mainly due to motor vehicle ***,there is no definite treatment for spinal cord *** To assess the outcome of rat embryonic stem cells(rESC)and autologous bone marrow-derived neurocytes(ABMDN)treatment in iatrogenic SCI created in rats,and to compare the efficacy of the two different cell *** The study comprised 45 male Wistar rats weighing between 250 and 300 g,which were divided into three groups,the control,rESC and ABMDN *** anesthetized animals underwent exposure of the thoracic 8th to lumbar 1st vertebrae.A T10-thoracic 12th vertebrae laminectomy was performed to expose the spinal cord.A drop-weight injury using a 10 g weight from a height of 25 cm onto the exposed spinal cord was *** wound was closed in *** urinary bladder was manually evacuated twice daily and after each evacuation Ringer lactate 5 mL/100 g was administered,twice daily after each bladder evacuation for the first 7 postoperative *** the 10th day,the rats underwent nerve conduction studies and behavioral assessment[Basso,Beattie,Brenham(BBB)]to confirm *** embryonic stem cells,ABMDN and saline were injected on the 10th *** animals were euthanized after 8 wk and the spinal cord was isolated,removed and placed in 2%formalin for histopathological analysis to assess the healing of neural tissues at the axonal *** All the animals tolerated the procedure *** BBB scale scoring showed that at the end of the first week no recovery was observed in the ***-injection,there was a strong and significant improvement in rats receiving rESC and ABMDN as compared to the control group based on the BBB scale,and the Trainof-four-Watch SX acceleromyography device exhibited statistically significant(Pspinal cord showed maximum vacuolization and least gliosis i

关键词： spinal cord injury Regeneration Embryonic stem cells Autologous bone marrow-derived neurocytes Iatrogenic Stem cells

spinal cord injury： potential neuroprotective therapy based on neural-derived peptides

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第11期11卷 1762-1763页

作者： elisa garcía josé mondragón-caso antonio ibarra Facultad de Ciencias de la Salud Universidad Anahuac Mexico Estado de Mexico Mexico

spinal cord injury （SCI） is a degenerative disease that causes an important neurological impairment, it is a medically complex and life-disrupting condition. SCI has a devastating impact on international health systems due to its irreversible consequences and lack of efficient treatment, affecting mostly young population within their productive years. Global SCI incidence in high-income countries is 40 to 80 new cases per million per year,

关键词： SCI spinal cord injury GME

Influence of spinal cord injury on core regions of motor function

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第3期16卷 567-572页

作者： Xiao-Yan Shen Chun-Ling Tao Lei Ma Jia-Huan Shen Zhi-Ling Li Zhi-Gong Wang Xiao-Ying Lü School of Information Science and Technology Nantong UniversityNantongJiangsu ProvinceChina Co-innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Institute of RF and OE-ICs Southeast UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Bioelectronics Southeast UniversityNanjingJiangsu ProvinceChina

Functional electrical stimulation is an effective way to rebuild hindlimb motor function after spinal cord ***,no site map exists to serve as a reference for implanting stimulator *** this study,rat models of thoracic spinal nerve 9 contusion were established by a heavy-impact method and rat models of T6/8/9 spinal cord injury were established by a transection *** microstimulation was performed to record motion types,site coordinates,and threshold currents induced by *** transection(complete injury),the core region of hip flexion migrated from the T13 to T12 vertebral segment,and the core region of hip extension migrated from the L1 to T13 vertebral *** was affected by post-transection time,but not transection ***,the longer the post-transection time,the longer the distance of *** study provides a reference for spinal electrode implantation after spinal cord *** study was approved by the Institutional Animal Care and Use Committee of Nantong University,China(approval No.20190225-008)on February 26,2019.

关键词： model motor neurological function rat recovery repair spinal cord injury

Single-atom cobalt nanozymes promote spinal cord injury recovery by anti-oxidation and neuroprotection

在线全文

学校读者我要写书评

暂无评论

Nano Research 2023年第7期16卷 9752-9759页

作者： Yuxing Jiang Hongtao Rong Yifan Wang Shange Liu Peng Xu Zhen Luo Lamei Guo Tao Zhu(✉) Hongpan Rong Dingsheng Wang Jiatao Zhang Yu Yi Hao Wang Tianjin Medical University General Hospital Tianjin 300052China CAS Center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials and NanosafetyNational Center for Nanoscience and TechnologyBeijing 100190China Beijing Key Laboratory of Construction-Tailorable Advanced Functional Materials and Green Applications Key Laboratory of Medical Molecule Science and Pharmaceutics EngineeringMinistry of Industry and Information TechnologySchool of Materials Science&EngineeringSchool of Chemistry and Chemical EngineeringInstitute of Engineering MedicineBeijing Institute of TechnologyBeijing 100081China Department of Chemistry Tsinghua UniversityBeijing 100084China University of Chinese Academy of Sciences Beijing 100049China

Oxidative stress and inflammation are central pathophysiological processes in a traumatic spinal cord injury(SCI).Antioxidant therapies that reduce the reactive oxygen and nitrogen species(RONS)overgeneration and inflammation are proved promising for improving the ***,efficient and long-lasting antioxidant therapy to eliminate multiple RONS with effective neuroprotection remains ***,a single-atom cobalt nanozyme(Co-SAzyme)with a hollow structure was reported to reduce the RONS and inflammation in the secondary injury of *** SAzymes featuring different single metal-N sites(e.g.,Mn,Fe,Co,Ni,and Cu),this Co-SAzyme showed a versatile property to eliminate hydrogen peroxide(H_(2)O_(2)),superoxide anion(O_(2)·^(-)),hydroxyl radical(·OH),nitric oxide(·NO),and peroxynitrite(ONOO^(-))that overexpressed in the early stage of *** porous hollow structure also allowed the encapsulation and sustained release of minocycline for neuroprotection in *** vitro results showed that the Co-SAzyme reduced the apoptosis and pro-inflammatory cytokine levels of microglial cells under oxidative *** addition,the Co-SAzyme combined with minocycline achieved remarkable improved functional recovery and neural repairs in the SCI-rat model.

关键词： single-atom catalysis spinal cord injury reactive oxygen and nitrogen species anti-inflammation neuroprotection

Synthesis and Characterization of a Silica-Based Drug Delivery System for spinal cord injury Therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2019年第2期11卷 61-80页

作者： Guodong Sun Shenghui Zeng Xu Liu Haishan Shi Renwen Zhang Baocheng Wang Changren Zhou Tao Yu College of Chemistry and Material Sciences Jinan UniversityGuangzhou 510632People’s Republic of China Department of Orthopedics First Affiliated HospitalJinan UniversityGuangzhou 510632People’s Republic of China College of Traditional Chinese Medicine Jinan UniversityGuangzhou 510632People’s Republic of China Tsinghua-Berkeley Shenzhen Institute Tsinghua UniversityShenzhen 518055People’s Republic of China

Acute inflammation is a central component in the progression of spinal cord injury(SCI).Anti-inflammatory drugs used in the clinic are often administered systemically at high doses,which can paradoxically increase inflammation and result in drug toxicity.A cluster-like mesoporous silica/arctigenin/CAQK composite(MSN-FC@ARCG)drug delivery system was designed to avoid systemic side effects of high-dose therapy by enabling site-specific drug delivery to the spinal *** this nanosystem,mesoporous silica was modified with the FITC fluorescent molecule and CAQK peptides that target brain injury and SCI *** size of the nanocarrier was kept at approximately 100 nm to enable penetration of the blood–brain ***,a Chinese herbal medicine,was loaded into the nanosystem to reduce *** in vivo results showed that MSN-FC@ARC-G could attenuate inflammation at the injury *** and morphology experiments suggested that MSN-FC@ARC-G could diminish local microenvironment damage,especially reducing the expression of interleukin-17(IL-17) and IL-17-related inflammatory factors,inhibiting the activation of astrocytes,thus protecting neurons and accelerating the recovery of *** study demonstrated that this novel,silica-based drug delivery system has promising potential for clinical application in SCI therapy.

关键词： Silica Drug delivery spinal cord injury Arctigenin Astrocytes

Purinergic signaling systems across comparative models of spinal cord injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2022年第3期17卷 2391-2398页

作者： Eva E.Stefanova Angela L.Scott Department of Pathology and Molecular Medicine McMaster UniversityHamiltonONCanada

Within the last several decades,the scientific community has made substantial progress in elucidating the complex pathophysiology underlying spinal cord ***,despite the many advances using conventional mammalian models,both cellular and axonal regeneration following spinal cord injury have remained out of *** this sense,turning to non-mammalian,regenerative species presents a unique opportunity to identify pro-regenerative cues and chara cterize a spinal cord microenvironment permissive to *** the signaling pathways hypothesized to be dysregulated during spinal cord injury is the purinergic signaling *** addition to its well-known role as energy currency in cells,ATP and its metabolites are small molecule neurotransmitte rs that mediate many diverse cellular processes within the central nervous *** our unde rstanding of the roles of the purinergic system following spinal cord injury is limited,this signaling pathway has been implicated in all injury-induced secondary processes,including cellular death,inflammation,reactive gliosis,and neural *** that the purinergic system is also evolutionarily conserved between mammalian and non-mammalian species,comparisons of these roles may provide important insights into conditions responsible for recovery ***,we compare the secondary processes between key model species and the influence of purinergic signaling in each *** our understanding of this signaling system and pro-regenerative conditions continues to evolve,so does the potential for the development of novel therapeutic interventions for spinal cord injury.

关键词： cell death differenriation glia inflammation neurogenesis proliferation purinergic signaling reactive gliosis regeneration spinal cord injury teleost

Surgical intervention combined with weight-bearing walking training improves neurological recoveries in 320 patients with clinically complete spinal cord injury:a prospective self-controlled study

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第5期16卷 820-829页

作者： Yansheng Liu Jia-Xin Xie Fang Niu Zhexi Xu Pengju Tan Caihong Shen Hongkun Gao Song Liu Zhengwen Ma Kwok-Fai So Wutian Wu Chen Chen Sujuan Gao Xiao-Ming Xu Hui Zhu Kunming International Spine and spinal cord injury Treatment Center Kunming Tongren HospitalKunmingYunnan ProvinceChina Clinical Center for spinal cord injury Kunming General Hospital of Chengdu Military CommandKunmingYunnan ProvinceChina Department of Laboratory Animal Sciences Shanghai Jiao Tong University School of MedicineShanghaiChina Department of Ophthalmology and State Key Laboratory of Brain and Cognitive Sciences The University of Hong KongHong Kong Special Administration RegionChina Guangdong-Hongkong-Macao Institute for Central Nervous System Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina Re-Stem Biotechnology Co.Ltd.SuzhouJiangsu ProvinceChina spinal cord and Brain injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Biostatistics Indiana University School of MedicineIndianapolisINUSA

Although a large number of trials in the SCI field have been conducted,few proven gains have been realized for *** the present study,we determined the efficacy of a novel combination treatment involving surgical intervention and long-term weight-bearing walking training in spinal cord injury(SCI)subjects clinically diagnosed as complete or American spinal injury Association Impairment Scale(AIS)Class A(AIS-A).A total of 320 clinically complete SCI subjects(271 male and 49 female),aged 16–60 years,received early(≤7 days,n=201)or delayed(8–30 days,n=119)surgical interventions to reduce intraspinal or intramedullary *** days post-surgery,all subjects received a weight-bearing walking training with the“Kunming Locomotion Training Program(KLTP)”for a duration of 6 *** neurological deficit and recovery were assessed using the AIS scale and a 10-point Kunming Locomotor Scale(KLS).We found that surgical intervention significantly improved AIS scores measured at 15 days post-surgery as compared to the pre-surgery baseline *** improvement of AIS scores was detected at 3 and 6 months and the KLS further showed significant improvements between all pair-wise comparisons of time points of 15 days,3 or 6 months indicating continued improvement in walking scores during the 6-month *** conclusion,combining surgical intervention within 1 month post-injury and weight-bearing locomotor training promoted continued and statistically significant neurological recoveries in subjects with clinically complete SCI,which generally shows little clinical recovery within the first year after injury and most are permanently *** study was approved by the Science and Research Committee of Kunming General Hospital of PLA and Kunming Tongren Hospital,China and registered at ***(Identifier:NCT04034108)on July 26,2019.

关键词： American spinal injury Association Impairment Scale–A functional recovery human intramedullary decompression spinal cord injury surgical intervention walking training

Cortical transcriptome analysis after spinal cord injury reveals the regenerative mechanism of central nervous system in CRMP2 knock-in mice

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第7期16卷 1258-1265页

作者： Ayaka Sugeno Wenhui Piao Miki Yamazaki Kiyofumi Takahashi Koji Arikawa Hiroko Matsunaga Masahito Hosokawa Daisuke Tominaga Yoshio Goshima Haruko Takeyama Toshio Ohshima Laboratory for Molecular Brain Science Department of Life Science and Medical BioscienceGraduate School of Advanced Science and EngineeringWaseda UniversityTokyoJapan Biomolecular Engineering Laboratory Department of Life Science and Medical BioscienceGraduate School of Advanced Science and EngineeringWaseda UniversityTokyoJapan Computational Bio Big-Data Open Innovation Laboratory(CBBD-OIL) National Institute of Advanced Industrial Science and Technology(AIST)TokyoJapan Research Organization for Nano and Life Innovation Waseda UniversityTokyoJapan Institute for Advanced Research of Biosystem Dynamics Waseda Research Institute for Science and EngineeringGraduate School of Advanced Science and EngineeringWaseda UniversityTokyoJapan Cellular and Molecular Biotechnology Research Institute National Institute of Advanced Industrial Science and Technology(AIST)TsukubaJapan Department of Molecular Pharmacology and Neurobiology Graduate School of MedicineYokohama City UniversityYokohamaJapan

Recent studies have shown that mutation at Ser522 causes inhibition of collapsin response mediator protein 2(CRMP2) phosphorylation and induces axon elongation and partial recovery of the lost sensorimotor function after spinal cord injury(SCI).We aimed to reveal the intracellular mechanism in axotomized neurons in the CRMP2 knock-in(CRMP2KI) mouse model by performing transcriptome analysis in mouse sensorimotor cortex using micro-dissection punching *** to that, we analyzed the structural pathophysiology in axotomized or neighboring neurons after SCI and found that somatic atrophy and dendritic spine reduction in sensorimotor cortex were suppressed in CRMP2KI *** analysis of the transcriptome has aided in the identification of four hemoglobin genes Hba-a1, Hba-a2, Hbb-bs, and Hbb-bt that are significantly upregulated in wild-type mice with concomitant upregulation of genes involved in the oxidative phosphorylation and ribosomal pathways after ***, we observed substantial upregulation in channel activity genes and downregulation of genes regulating vesicles, synaptic function, glial cell differentiation in CRMP2KI ***, the transcriptome profile of CRMP2KI mice has been discussed wherein energy metabolism and neuronal pathways were found to be differentially *** results showed that CRMP2KI mice displayed improved SCI pathophysiology not only via microtubule stabilization in neurons, but also possibly via the whole metabolic system in the central nervous system, response changes in glial cells, and *** together, we reveal new insights on SCI pathophysiology and the regenerative mechanism of central nervous system by the inhibition of CRMP2 phosphorylation at *** these experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee at Waseda University, Japan(2017-A027 approved on March 21, 2017;2018-A003 approved on March 25, 2018;2019-A026 approved on March

关键词： CNS regeneration cortex CRMP2 hemoglobin metabolic pathway spinal cord injury spine transcriptome

Photo-responsive Carboxymethyl Chitosan/Laponite Hydrogel as a Potential spinal cord injury Scaffold:Characterization and Cytocompatibility Study

在线全文

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2024年第6期39卷 1628-1636页

作者： Jayanti Parajuli LI Yongtao CHANG Likun YE Liyuan HAN Yingchao YIN Yixia State Key Laboratory of Advanced Technology for Material Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Biomedical Materials and Engineering Research Center of Hubei Province Wuhan University of TechnologyWuhan 430070China

We synthesized photo-responsive carboxymethyl chitosan(CMC-MA)via free radical polymerization and utilized nanoclay laponite(LAP)as an inorganic crosslinking agent to develop an injectable and 3D-printable CMC-MA/LAP *** determined the optimal ratio of 2.5 w/v%CMC-MA/7.5 w/v%LAP based on injection molding,compression modulus,swelling properties,rheological properties,and 3D printing properties of the hydrogel ***-vitro cytocompatibility experiments showed that both CMC-MA and CMC-MA/LAP hydrogel had no inhibitory effect on cell proliferation and can promote cell growth when cultured on the surface of the hydrogel ***,the hydrogel containing LAP particles significantly facilitated cell adhesion(>60%)compared with the hydrogel without LAP(20%).Our findings demonstrate that the CMC-MA/LAP hydrogel has great potential for tissue repair in neural tissue engineering.

关键词： neural injectable hydrogels spinal cord injury CMC-MA/LAP hydrogel cytocompatibility