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Combined remediation of DDT congeners and cadmium in soil by sphingobacterium sp.D-6 and Sedum alfredii Hance

Journal of Environmental Sciences 2012年第6期24卷 1036-1046页

作者： Hua Fang Wei Zhou Zhengya Cao Feifan Tang Dandan Wang Kailin Liu Xiangwei Wu Xiao'e Yang Yongge Sun Yunlong Yu Department of Plant Protection College of Agriculture & Biotechnology Zhefiang University Hangzhou 310058 China. E-mail: agri@*** Key Laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture Hangzhou 310058 China MOE Key Lab of Environment Remediation & Ecosystem Health College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 China Department of Earth Science Zhejiang University Hangzhou 310027 China State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China

Combined pollution of 1,1,1-trichloro-2,2-bis （4-chlorophenyl） ethane （DDT） and cadmium （Cd） in agricultural soils is of great concern because they present serious risk to food security and human *** order to develop a cost-effective and safe method for the removal of DDTs and Cd in soil,combined remediation of DDTs and Cd in soil by sphingobacterium sp.D-6 and the hyperaccumulator,Sedum alfredii Hance was *** treatment for 210 days,the degradation half-lives of DDTs in soils treated by strain D-6 decreased by 8.1% to 68.0% compared with those in the *** inoculation of strain D-6 into soil decreased the uptake of DDTs by pak choi and *** shoots/roots ratios of *** for the Cd accumulation ranged from 12.32 to *** Cd concentration in soil decreased to 65.8%-71.8% for *** treatment and 14.1%-58.2% for *** and strain D-6 combined treatment,resp.ctively,compared with that in the *** population size of the DDTs-degrading strain,Simpson index （1/D） and soil resp.ratory rate decreased in the early stage of treatment and then gradually increased,ultimately recovering to or exceeding the initial *** results indicated that synchronous incorporation of strain D-6 and *** into soil was found to significantly （p 0.05） enhance the degradation of DDTs in soil and the hyperaccumulation of Cd in *** was concluded that strain D-6 and *** could be used successfully to control DDTs and Cd in contaminated soil.

关键词： bioremediation cadmium sphingobacterium sp. Sedum alfredii Hance 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane

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Mass sp.ctrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3, Shaoxiang XIONG2, Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China 2Center of Mass sp.ctrometry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China 3Graduate School of the Chinese Academy of Sciences, Beijing 100039, China Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China Graduate School of the Chinese Academy of SciencesBeijing 100039 China Center of Mass sp.ctrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the sp.cies sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass sp.ctrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da resp.ctively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might sp.cifically cut the sites with low sulfated domain in heparin.

关键词： electrosp.ay ionization-mass sp.ctrometry heparin disaccharides heparinase high perfor- mance liquid chromatography sphingobacterium sp.

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