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Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles

Burns & Trauma 2021年第1期9卷 475-487页

作者： Yijie Zhang Enhejirigala Bin Yao Zhao Li Wei Song Jianjun Li Dongzhen Zhu Yuzhen Wang Xianlan Duan Xingyu Yuan Sha Huang Xiaobing Fu Research Center for Tissue Repair and Regeneration Medical Innovation Research Department and the Fourth Medical CenterChinese PLA General Hospital and PLA Medical CollegeBeijing 100048China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationChinese PLA General Hospital and PLA Medical CollegeBeijing 100853China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China College of Graduate Tianjin Medical UniversityTianjin 300070China Institute of Basic Medical Research Inner Mongolia Medical UniversityHohhot 010110Inner MongoliaChina The Shenzhen Key Laboratory of Health Sciences and Technology Graduate School at ShenzhenTsinghua UniversityShenzhen 518055GuangdongChina Department of General Surgery the Sixth Medical CenterChinese PLA General HospitalBeijing 100048China Department of Burn and Plastic Surgery Air Force Hospital of Chinese PLA Central Theater CommandDatong 037000ShanxiChina School of Medicine Nankai UniversityTianjin 300071China

Background:Sweat glands(SGs)and hair follicles(HFs)are two important cutaneous appendages that play crucial roles in homeostatic maintenance and thermoregulation,and their interaction is involved in wound *** can be regenerated from mesenchymal stem cell-laden 3D bioprinted scaffolds,based on our previous studies,whereas regeneration of HFs could not be achieved in the same *** to the lack of an in vitro model,the underlying molecular mechanism of the interaction between SGs and HFs in regeneration could not be fully *** purpose of the present study was to establish an in vitro model of skin constructs with SGs and HFs and explore the interaction between these two appendages in ***:To investigate the interaction effects between SGs and HFs during their regeneration processes,a combined model was created by seeding HF spheroids on 3D printed SG *** interaction between SG scaffolds and HF spheroids was detected using RNA expression and immunofluorescence *** effects of microenvironmental cues on SG and HF regeneration were analysed by altering seed cell types and plantar dermis homogenate in the ***:According to this model,we overcame the difficulties in simultaneously inducing SG and HF regeneration and explored the interaction effects between SG scaffolds and HF ***,HF spheroids promoted both SG and HF differentiation in SG scaffolds,while SG scaffolds promoted SG differentiation but had little effect on HF potency in HF ***,microenvironmental factors(plantar dermis homogenate)in SG scaffolds effectively promoted SG and HF genesis in HF spheroids,no matter what the seed cell type in SG scaffolds was,and the promotion effects were ***:Our approach elucidated a new model for SG and HF formation in vitro and provided an applicable platform to investigate the interaction between SGs and HFs in *** platform might facilitate 3D skin con

关键词： Skin regeneration Sweat glands Hair follicle 3D bioprinting spheroid culture Skin constructs

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Breast Cancer MCF-7 Cell spheroid culture for Drug Discovery and Development

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Journal of Cancer Therapy 2022年第3期13卷 117-130页

作者： Guangping Chen William Liu Bingfang Yan Department of Physiological Sciences College of Veterinary Medicine Oklahoma State University Stillwater OK USA College of Pharmacy University of Cincinnati Cincinnati OH USA

In vitro 3D cancer spheroids (tumoroids) exhibit a drug resistance profile similar to that found in solid tumors. 3D spheroid culture methods recreate more physiologically relevant microenvironments for cells. Therefore, these models are more appropriate for cancer drug screening. We have recently developed a protocol for MCF-7 cell spheroid culture, and used this method to test the effects of different types of drugs on this estrogen-dependent breast cancer cell spheroid. Our results demonstrated that MCF-7 cells can grow spheroid in medium using a low attachment plate. We managed to grow one spheroid in each well, and the spheroid can grow over a month, the size of the spheroid can grow over a hundred times in volume. Our targeted drug experimental results suggest that estrogen sulfotransferase, steroid sulfatase, and G protein-coupled estrogen receptor may play critical roles in MCF-7 cell spheroid growth, while estrogen receptors α and β may not play an essential role in MCF-7 spheroid growth. Organoids are the miniatures of in vivo tissues and reiterate the in vivo microenvironment of a specific organ, best fit for the in vitro studies of diseases and drug development. Tumoroid, developed from cancer cell lines or patients’ tumor tissue, is the best in vitro model of in vivo tumors. 3D spheroid technology will be the best future method for drug development of cancers and other diseases. Our reported method can be developed clinically to develop personalized drugs when the patient’s tumor tissues are used to develop a spheroid culture for drug screening.

关键词： MCF-7 Cell spheroid culture 3D Cell culture Estrogen-Dependent Breast Cancer Cancer Drug Development Personalized Cancer Drug Development

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