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Guijiajiao(Colla Carapacis et Plastri,CCP)prevents male infertility via gut microbiota modulation

Chinese Journal of Natural Medicines 2023年第6期21卷 403-410页

作者： SHENG Wen XU Wenjing DING Jin LU Baowei LIU Lumei HE Qinghu ZHOU Qing College of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410000China Andrology Laboratory Hunan University of Chinese MedicineChangsha 410000China Department of Dermatology The First Affiliated Hospital of Hunan University of Chinese MedicineChangsha 410000China Department of Andrology Affiliated Bao'an Hospital of Traditional Chinese MedicineThe Seventh Clinical Medical CollegeGuangzhou University of Traditional Chinese MedicineShenzhen 518000 College of Integrated Traditional Chinese and Western Medicine Hunan University of Chinese MedicineChangsha 410000 College of Traditional Chinese Medicine Hunan University of MedicineChangsha 410000 Department of Andrology The First Hospital of Hunan University of Chinese MedicineChangsha 410000

Male infertility is a significant cause of psychosocial and marital distress in approximately 50% of couples who are unable to conceive,with male factors being the underlying cause.Guijiajiao(Colla Carapacis et Plastri,CCP)is a Traditional Chinese Medicine commonly used to treat male infertility.The present study aimed to investigate the potential mechanisms underlying the preventive effects of CCP on male infertility.An infertile male rat model was established using cyclophosphamide(CTX),and CCP was administered for both treatment and prevention.Fecal microbiota transplantation(FMT)was also performed to explore the role of gut microbiota in the CCP-mediated prevention of male infertility in rats.Sperm motility and concentration were determined using a semiautomatic sperm classification analyzer.Subsequently,histopathological analysis using HE staining was performed to examine the changes in the small intestine and testis.Moreover,the serum levels of lipopolysaccharide(LPS)and testosterone were measured by ELISA.In addition,immunohistochemistry was conducted to detect CD3 expression in the small intestine,while RT-qPCR was employed to assess the expressions of interleukin-1 beta(IL-1β),cluster of differentiation 3(CD3),Monocyte chemoattractant protein-1(MCP-1),and C-X-C motif chemokine ligand 10(CXCL-10)in the small intestine and epididymis.Finally,gut microbiota was analyzed by 16S rRNA sequencing.CCP improved sperm motility,number,and concentration in CTX-induced infertile male rats.CCP increased the serum testosterone level,inhibited the immune cell infiltration of the intestinal lamina propria,and promoted the aggregation of CD3+T cells in CTX-induced male infertility rats.CCP also inhibited the expressions of MCP-1,CXCL-10,and IL-1βin the epididymis of male infertility rats.At the genus level,CTX led to a reduction in the abundance of Lactobacillus,Clostridia_UCG.014,and Romboutsia in the intestinal tract of rats.In contrast,CCP decreased the abundance of Ruminococcus and increased the abundance of Romboutsi

关键词： Colla Carapacis et Plastri Fecal microbiota transplantation Male infertility Gut microbiota spermatogenesis ability

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