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Preparation of Anti-HER2 Monoclonal Antibody-paclitaxel Immunoconjugate and Its Biological Evaluation

Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第6期31卷 735-740页

作者：刘东徐艳娇饶子超陈兆聪 Department of Pharmacy Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Biochemistry & Molecular Biology Tongji Medical College Huazhong University of Science and Technology

Anti-HER2 monoclonal antibody （Sc7301）-paclitaxel （TAX） immunoconjugate was pre- pared and its specific binding to tumor cells was investigated in this study. Sc7301 was conjugated to TAX by the active ester method and then the TAX-Sc7301 immunoconjugate was obtained. After purification and labeling by Cyano-fluorescein isothiocyanate （FITC）, the specific binding of TAX-Sc7301 to HER2-positive tumor cells （SKOV3） and HER2-negative tumor cells （HepG2） was evaluated respectively. TAX-Sc7301 （20 nmol/L） showed distinct specific binding to SKOV3 cells rather than HepG2 cells. And the uptake of the immunoconjugate by SKOV3 cells was increased with the TAX-So7301 concentration （3-48 nmol/L） and the incubation time （P〈0.05）. It was concluded that the TAX-Sc7301 immunoconjugate is ootentially applicable as a targeted agent against HER2-10ositive tumor cells.

关键词： paclitaxel anti-HER2 monoclonal antibody immunoconjugate specific binding

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Sample preparation method to improve the efficiency of high-throughput single-molecule force spectroscopy

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Biophysics Reports 2019年第4期5卷 176-183页

作者： Lei Jin Li Kou Yanan Zeng Chunguang Hu Xiaodong Hu State Key Laboratory of Precision Measuring Technology & Instruments Tianjin University Nanchang Institute for Microtechnology of Tianjin University College of Engineering and Technology Tianjin Agricultural University

Inefficient sample preparation methods hinder the performance of high-throughput single-molecule force spectroscopy(H-SMFS) for viscous damping among reactants and unstable linkage. Here, we demonstrated a sample preparation method for H-SMFS systems to achieve a higher ratio of effective target molecules per sample cell by gas-phase silanization and reactant hydrophobization. Digital holographic centrifugal force microscopy(DH-CFM) was used to verify its performance. The experimental result indicated that the DNA stretching success ratio was improved from 0.89% to 13.5%. This enhanced efficiency preparation method has potential application for force-based DNA stretching experiments and other modifying procedures.

关键词： specific binding Digital holographic centrifugal force microscopy Sample preparation High-throughput single-molecule force spectroscopy

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