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Edwardsiella tarda and Aeromonas hydrophila isolated from diseased southern flounder(Paralichthys lethostigma)are virulent to channel catfish and Nile tilapia

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Journal of Coastal Life Medicine 2014年第5期 337-343页

作者： Julia W.Pridgeon Phillip H.Klesius Gregory A.Lewbart Harry V.Daniels Megan Jacob Aquatic Animal Health Research Unit USDA-ARS990 Wire RoadAuburnAL 36832United States of America College of Veterinary Medicine North Carolina State University1060 William Moore DriveRaleighNC 27607United States of America Department of Applied Ecology North Carolina State UniversityRaleighNC 27695United States of America

Objective:To identify bacteria isolated from diseased southern flounder and determine whether they are virulent to channel catfish and Nile ***:Gram-negative bacteria isolates were recovered from five tissues of diseased southern flounder(Paralichthys lethostigma).The isolates were subjected to biochemical and molecular identification followed by virulence study in ***:Based on biochemical analysis,the 25 isolates were found to share homologies with either Edwardsiella tarda(***)or Aeromonas hydrophila(***).Based on sequencing results of partial 16S rRNA gene,15 isolates shared 100%identities with the 16S rRNA sequence of previously identified *** strain TX1,whereas the other 10 isolates shared 100%identities with the 16S rRNA sequence of previously identified *** strain *** healthy fish were exposed to flounder isolate by intracoelomic injection,the LD50 values of flounder isolate *** to channel catfish or Nile tilapia[(10±2)g]were 6.1×10^(4)and 1.1×10^(7)CFU/fish,respectively,whereas that of flounder isolate *** to channel catfish and Nile tilapia were 1.4×10^(7)and 5.6×10^(7)CFU/fish,***:This is the first report that *** and *** isolated from diseased southern flounder are virulent to catfish and tilapia.

关键词： Edwardsiella tarda Aeromonas hydrophila southern flounder Pathogen Virulence

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Induction of diploid gynogenesis in southern flounder Paralichthys lethostigma with left-eyed flounder Paralichthys olivaceus sperm

Induction of diploid gynogenesis in southern flounder Parali...

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江苏省遗传学会第七届二次代表大会暨学术研讨会

作者： Jian-He Xu1,2,, Feng You2 , Bin-Lun Yan1,Pei-Jun Zhang2, Bi-Xiang Jing1 (1Huaihai Institute of Technology, Key Laboratory of Marine Biotechnology of Jiangsu Province, Lianyungang 222005,China 2 Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China

＜正＞southern flounder Paralichthys lethostigma exhibits sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males.

关键词： southern flounder Paralichthys oliveaceus heterologous sperm gynogenesis cold shock

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