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Preliminary　Research　on　the　p53　Gene　Rearrangements　in　the　Evolution　of　Chronic　Myelogenous　Leukemia　to　Blast　Crisis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1994年第4期14卷 204-208页

作者：陈敬春刘树茂费洪宝龚维龙 Research Unit of Hematological Genetics Institute of Hematology Xiehe Hospital Tongji Medical University Wuhan

DNA from 36 patients with chronic myelogenous leukemia (CML) at various clinical stages and 6 cases of acute leukemia was investigated for alterations of the p53 gene by southern blot *** of the p53 gene were seen in 3 of 12 (25.00%) cases of blast crisis and accelerated phase (AP) of CML and in only one of 18 chronic phrase (CP),just as has been reported previously. Meanwhile,by restriction fragment length polymorphism (RFLP) analysis the Bgl II site polymorphism in the p53 gene was also found. The frequency in Chinese people detected here was 0.392,which was strikingly higher than that in some other countries(P<0. 001).These results suggested that the alterations of the p53 gene, for example,p53 rearrangements,were probably responsible for the progression of BC in some CML patients, and that the frequency of Bgl II polymorphism in the p53 gene might be related to the population distribution.

关键词： chronic myelogenous leukemia blast crisis p53 gene southern blot analysis restriction fragment length polymorphism.

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Preliminary Study on bcr Rearrangement in Leukemia

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1994年第1期14卷 20-23页

作者：金润铭杨爱德费洪宝 Research Unit of Hematological Genetics Institute of Hematology Xiehe Hospital Tongji Medical University Wuhan

southern blot analysis was conducted in 15 patients by using a 1.2 Kb Hind 1 Ⅲ/Bgl Ⅱfragment from the 3'end of the bcr region and a 2.0 Kb Bgl Ⅱ/Hind Ⅲ fragment from the 5' end of the bcr as *** the 15 patients in our group,11 with chronic myelocytic leukemia (CML),3 with Ph-negative acute lymphocytic leukenma(ALL).one with myelodysplastic syndrome ZMDS).Bcr rearrangements were detected in 9 patients with CML and were negative in the rest of the *** results showed that the identification of bcr rearrangement was very important in the diagnosis of Ph-positive leukemias.

关键词： southern blot analysis bcr rearrangement leukemia

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Transgenic Date Palm Containing Endotoxin Cry3Aa Gene

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Journal of Agricultural Science and Technology(A) 2017年第4期7卷 246-257页

作者： Awatef Mahmoud Badr-Elden Ibrahim Abd El-Maksoud Ibrahim Hamdy Ahmed Emaral Mahdia Farid Gabr Ahmed Abbas Nower Tamer Mahfouz Abd Elaziem Department of Plant Biotechnology Genetic Engineering and Biotechnology Research Institute (GEBRI) University of Sadat CitySadat City 32897 Egypt Department of Plant Genetic Resources Desert Research Center Cairo 11753 Egypt

Date palm, like all other crops, is very sensitive to the injury by many insect pests, which may lead to the death of the affected plant and causes decrease in yield. In the present study, an efficient Agrobacterium for genetic transformation was successfully achieved for well known date palm （Phoenix dactylifera L.） cv. Medjool and Khalas using callus as explant. Embryogenic callus were recorded 100% mortality when cultured on MS medium containing 100 mg/L kanamycin with different cultivars, thus it was chosen for the selection of transformed explants. Embryogenic callus of Medjool and Khalas were incubated with Agrobacterium tumefaciens strain LBA 4404 for 0.5, 1, 2, 4 and 24 h on LB medium. After the incubation periods, embryogenic callus was transferred to MS medium with 0.1 mg/L NAA, 0.05 mg/L BA, 250 mg/L carbenicillin and 100 mg/L kanamycin for detection of transgenic embryogenic callus. Polymerase chain reaction （PCR） was used for the rapid screening of Cry3Aa gene. For screening, total genomic DNA was isolated from transformants. Using primer specific to Cry3Aa gene （forward and reverse）, a PCR product with a size of about 2,000 bp was amplified when all nucleic acid from the transformants were utilized as templates. PCR analysis confirmed the appearance of the transgene of 2,000 bp in one individual plantlet. Presence and integration of foreign Cry3Aa gene in regenerated kanamycin resistant embryogenic callus was also confirmed by southern blot hybridization. It was found that one transgenic embryogenic callus for both Medjool and Khalas showed a single copy of gene integration. These results signify the successful transfer of Cry3Aa gene into date palm plant.

关键词： Date palm Agrobacterium tumefaciens Cry3Aa gene southern blot analysis transformation.

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