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sophocarpine attenuates liver fibrosis by inhibiting the TLR4 signaling pathway in rats

World Journal of Gastroenterology 2014年第7期20卷 1822-1832页

作者： Hui Qian Jian Shi Ting-Ting Fan Jiao Lv Si-Wen Chen Chun-Yan Song Zhi-Wu Zheng Wei-Fen Xie Yue-Xiang Chen Department of Gastroenterology Shanghai Changzheng HospitalSecond Military Medical University Department of Gastroenterology the 411~(th) Hospital of PLA

AIM: To explore the effect of sophocarpine on experimental liver fibrosis and the potential mechanism involved.

关键词： Liver fibrosis sophocarpine Toll-like receptor 4 Hepatic stellate cells Cytokines

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Protection of sophocarpine on Colonic Barrier in DSS-induced Acute Colitis in Mice by Increasing Expression of HNF4α

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Chinese Herbal Medicines 2015年第3期7卷 261-266页

作者： Jian-mei Zhang Ya-bi Zhu Hong-guang Li Shuang-mei Luan Chun-yan Song Xing Deng Yue-xiang Chen Department of Gastroenterology Lishui People's Hospital Department of Gastroenterology The Fourth Affiliated Hospital of Zhejiang University School of Medicine Department of Gastroenterology Changzheng Hospital Second Military Medical University

Objective To investigate the possible protective effects of sophocarpine on mucosal injury and epithelial barrier disruption on dextran sulfate sodium （DSS）-induced acute colitis. Methods Male BALB/c mice were randomly divided into three groups. The mice in normal group were given normal water, and those in model and sophocarpine- treated groups were given 2.5% DSS for 6 d to induce acute colitis. sophocarpine （30 mg/kg） was ip administered once daily during the study period. Severity of colitis was evaluated by disease activity index （DAI）, histological injury and inflammatory cytokine production including tumor necrosis factor-α （TNF-α）, interleukin-lβ （IL-Iβ）, and monocyte chemoattractant protein-1 （MCP-1）. The colonic barrier disruption was assessed by testing the expression of junctional adhesion molecule-1 （JAM-1）, E-cadherin （E-CAD）, and desmocollin-2 （DSC-2） in colon mucosa. Expression of HNF4~ in colon mucosa was detected by immunohistochemistry staining and real-time RT-PCR, respectively. Results Compared with normal group, DAI, colonic shortening, and histopathological injury in model group were elevated （P 〈 0.05）, but reduced in sophocarpine-treated group （P 〈 0.05）. Compared with model group, the mRNA expression of inflammatory cytokines （TNF-α, IL-16, MCP-1） were obviously lower in sophocarpine-treated group （P 〈 0.05）, while the cellular junction proteins （E-CAD, JAM-l, and DSC-2） were higher （P 〈 0.05）. The expression of HNF4α at mRNA and protein levels was decreased significantly in model group, but increased apparently in sophocarpine-treated group. Conclusion sophocarpine can enhance the expression of HNF4α, promote the expression of colonic intrecellular junctions, thus, maintain the integrity of the colonic barrier and inhibit the colitis process. We suggest that sophocarpine could enhance the production of cellular junction proteins to protect the intestinal barrier fuction, at least partly, in HNF4α-dependent pathway.

关键词： colitis dextran sulfate sodium hepatocyte nuclear factor 4α inflammatory cytokines sophocarpine

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PROTECTIVE ROLE OF sophocarpine IN COXSACKIEVIRUS B3 INFECTION IN CULTURED RAT CARDIOMYOCYTES

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Journal of Shanghai Second Medical University(Foreign Language Edition) 2007年第1期19卷 65-68页

作者：张星宇陈曙霞钱富荣刘晶星 Department of Emergency Renji Hospital School of Medicine Shanghai Jiaotong University Department Cardiovascular Research Renji Hospital School of Medicine Shanghai Jiaotong University Department of Microbiology School of Medicine Shanghai Jiaotong University

Objective To observe the anti-CVB3 （ Coxsackievirus B3 ） effect of sophocarpine （SC） extracted from Sophora flavescens, a traditional Chinese herb in vitro. Methods Cardiomyocytes from the neonatal rat were cultured to establish the viral myocarditis model The cells were divided into four groups： infected group （ infected by CVB3 ） , SC treated group （ added SC 100 μg/mL after viral infection ）, SC control group （ added SC 100 μg/mL only）, and normal control group. The cytopathic effect （CPE） and the beating frequency of the myocardial cells were observed and the LDH levels in the supernatant were measured at day 2,3, and 5. The cultured myocytes were added different concentrations of SC （ 12. 5 -400 μg/mL ） after infection with CVB3, the CPE was observed and the concentrations of LDH were measured and compared at day 2, 3, and 5. Results In the SC treated group （ 100 μg/mL ） , the cytopathic effect was lighter and the LDH level was lower than the infected group. SC in a concentration of 12. 5 - 300 μg/mL could relieve the CPE and lower the LDH level, while in a higher concentration （400 μ/m ） , it exacerbated the CPE caused by the virus, and the LDH levels were higher than the infected cells. Conclusion SC in certain concentration could protect the cultured rat cardiomyocytes from CVB3 infection.

关键词： sophocarpine coxsackievirus B3 rat beating cardiac myocytes

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