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somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第4期7卷 291-298页

作者： RAO Abdul Qayyum HUSSAIN S. Sarfraz SHAHZAD M. Saqib BOKHARI S. Yassir Abbas RAZA M. Hashim RAKHA Allah MAJEED A. SHAHID A. Ali SALEEM Zafar HUSNAIN Tayyab RIAZUDDIN S. Institution Centre of Excellence in Molecular Biology University of the Punjab Lahore 53700 Pakistan

Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypo-cotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.

关键词： Cotton Callus somatic embryogenic Wild species Cell suspension culture

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Studies on somatic Embryogenesis and Its Cryopreservation of Litchi

Studies on Somatic Embryogenesis and Its Cryopreservation of...

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2005年全国植物生长物质研讨会

作者： Yuming Xie , Ganjun Yi, Qiuming Zhang( College of Horticulture, Hunan Agricultural University, Hunan, Changsha 410128 Fruit Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou 510640)

＜正＞Litchi（Litchi chinesis Sonn）of the family Sapindaceae is a subtropical tree known for itsdelicious *** it had difficulty in vitro *** are few reports on somaticembryogenesis and germplasm *** experiment was conducted about somaticembryogenesis and its cryopreservation of litchi by vitrification The aim is to establishprocedure of somatic embryogenesis and its cryopreservation,which it will benefit to litchiresearch of biological technique and germplasm *** main research result is asfollows:

关键词： Litchi somatic embryogenic Cryopreservation Vitrification

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