somatic embryogenesis is a very efficient way to propagate economically important plants;however, not all genotypes within a species can be propagated using this method, as a combined effect of both genetic and epigen...
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somatic embryogenesis is a very efficient way to propagate economically important plants;however, not all genotypes within a species can be propagated using this method, as a combined effect of both genetic and epigenetic mechanisms may be involved in the response. The aim of the present study was to perform a comparative analysis of the genetic differences through amplified fragment length polymorphism (AFLP) and the epigenetic differences through methylation-sensitive amplified polymorphism (MSAP) of two Agave fourcroydes clonal lines, one highly embryogenic (K33) and the other non-embryogenic (K7). Genetic and epigenetic variabilities existed within each clonal line;however, the polymorphic profiles from the two marker systems allowed us to clearly distinguish the two clonal lines before somatic embryogenesis induction. During the induction, the changes detected were mainly 1) unmethylated fragments in the initial explants that were methylated during induction (methylation events) and 2) fragments with different methylation states in the initial explant that were unmethylated in some stages of the process (demethylation events). K33 showed greater dynamism in relation to methylation/demethylation events, while K7 presented the methylation events in a more constant range and at higher levels during all process.
<正>Study on in vitro cultures of ten cassava cultivars (KU50、TME7、Ebwanateraka、NZ188、NZ199、SC5、SC6、SC8、SC124 and SC205) collected from China and Africa demonstrated the significant different
<正>Study on in vitro cultures of ten cassava cultivars (KU50、TME7、Ebwanateraka、NZ188、NZ199、SC5、SC6、SC8、SC124 and SC205) collected from China and Africa demonstrated the significant different
Protoplasts of embryogenic suspension cells of loblolly pine (Pinus taeda L).were isolated at exponential growth *** of various concentrations of basal medium,levels of BA,and concentrations of inositol on the d...
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Protoplasts of embryogenic suspension cells of loblolly pine (Pinus taeda L).were isolated at exponential growth *** of various concentrations of basal medium,levels of BA,and concentrations of inositol on the differentiation of embryonal suspensor mass (ESM),early stage somatic embryos (ESE) ,and lae stage somatic embryos (LSE) were investigated .A study of the effect of various concentrations of LP basal medium sowed that the optimal basal medium concentration of ESM,ESE,and LSE differentiation was 1.25 LP *** effects of various levels of BA and inositol showed that the optimal concentrations of BA for the formation of ESM,ESE and LSE were 4 mg/L ,2mg/L and 1mg/L,respectively ,and the optimal concentrations of inositol for the ESM ,ESE and LSM formation were 400mg/L,800mg/L and 1,200mg/L,respectively.
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