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sodium 4-phenylbutyrate Attenuates High-fat Diet-induced Impaired Spermatogenesis

Biomedical and Environmental Sciences 2018年第12期31卷 876-882页

作者： WANG Er Hui YAO San Qiao TAO Ling XI Jin Yan Department of Nutrition and Food Hygiene College of Public Health Xinxiang Medical University Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine Xinxiang Medical University

Objective To determine the mitigating effects of sodium 4-phenylbutyrate(4-PBA) on high-fat diet(HFD)-induced spermatogenesis dysfunction. Methods Male rats(n = 30) were randomly divided into three groups: control, HFD, and 4-PBA(HFD +4-PBA). After 13 weeks, rats were euthanized. Testes and epididymis were harvested for further analysis. Sex hormones were detected, and hematoxylin and eosin staining was performed to examine the histological changes in the testes. Semen samples were collected to evaluate sperm quality. Spermatogenic cell apoptosis was detected by TUNEL assay. Results Compared with the control group, the final body weight and body weight gain were significantly higher in HFD-fed rats, while the testicle/body weight ratios were lower(P 4-PBA attenuated HFD-induced histological damage, ameliorated the HFD-induced decrease in serum testosterone(T), and reduced the rate of testicular cell apoptosis(P 4-PBA significantly improved semen parameters in HFD rats(P 4-PBA ameliorated HFD-induced impaired spermatogenesis via inhibition of apoptosis in rats. 4-PBA may have therapeutic value in the treatment of obesity-related impairment of spermatogenesis.

关键词： Male infertility sodium 4-phenylbutyrate Obesity Spermatogenesis Semen Testis

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Growth inhibition and gene induction in human hepatocellular carcinoma cell exposed to sodium 4-phenylbutanoate

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Chinese Medical Journal 2008年第17期121卷 1707-1711页

作者： WANG Chun-ting MENG Mei ZHANG Ji-cheng JIN Chang-jun JIANG Jin-jiao REN Hong-sheng JIANG Jun-mei QIN Cheng-yong YU Dong-qing Department of Medical Intensive Care Unit Department of Digestive Diseases Shandong Province Hospital Affiliated Hospital of Shandong University Jinan Shandong 250021 China

Background sodium 4-phenylbutanoate （NaPB） can induce cellular differentiation and cell cycle arrest. However, its potential anticancer properties in hepatocellular carcinoma and influence on normal liver cell are still unclear. We observed the effects of NaPB on growth inhibition, including differentiation and phase growth arrest in normal liver cell line L-02 and hepatocellular carcinoma cell line Bel-7402. Furthermore, we investigated its mechanism in Bel-7402. Methods Hepatocellular carcinoma cells Bel-7402 and normal liver cell line L-02 were treated with NaPB at different concentrations. Light microscopy was used to find morphological change in cells. Cell cycle was detected by flow cytometry. Expression of acetylating histone H4 and of histones deacetylase 4 （HDAC4） were determined by Western blot. The expression of P21WAF1/CIP1 and E-cadherin were observed through immunocytochemistry. Results NaPB treatment led to time dependent growth inhibition in hepatocellular carcinoma cells Bel-7402. NaPB treatment caused a significant decline in the fraction of S phase cells and a significant increase in Go/G1 cells. NaPB increased the expression of P21wAFVCIP1 and E-cadherin in Bel-7402 and significantly decreased the level of HDAC4 in Bel-7402. NaPB significantly improved the level of acetylating histone H4. The normal liver cell line L-02 showed no distinct changes under treatment with NaPB. Conclusions NaPB inhibited the growth of hepatocellular carcinoma cells Bel-7402 and induced partial differentiation through enhancing the acetylating histones. In Bel-7402, the expressions of P21WAF1/CIP1 and E-cadherin may be related to level of acetylating histones and inhibition of cellular growth. NaPB showed no significant effect on normal liver cells.

关键词： sodium 4-phenylbutyrate histones hepatocellular carcinoma cell line

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