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Effect of siRNA interference on nerve growth factor in intervertebral disc inflammation rats

Asian Pacific Journal of Tropical Medicine 2014年第9期7卷 739-743页

作者： Ming-Lei Lang Ai-Lin Qin Jian-Min Li Peng Fu Department of Orthopedics Qilu Hospital Affiliated to Shandong University Shengli Oilfield Central Hospital Department of Orthopedics Gaomi People's Hospital

Objective:To investigate the inhibition effect of siRNA interference on NGF induced by inflammatory factor IL-6,and JUL—1 so as to provide novel targets for clinical treatment of discogenic low back ***:The intervertebral disc nucleus and annulus fibrosus cells of rats were separated-The cells were co-cultured with different concentrations(10 nmol/L,20nmol/L,50 nmol/L,100 nmol/L)of IL-6 and IL-1β.The NGF-siRNA was leaded into the cocultured cells with its import ability assessed by flow cytometry instrument tests,hefore and after which the NCF mRNA expression was detected by real-time Q-PCR and the NGF content was detected by ***:Flow cytometry instrument test results showed that the NGFsiRNA cell conversion rate was 99.8%.Real-time Q-PCR detection results showed that compared with negative control group,the NGF mRNA expression of co-cultured cells treated by 10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/L IL-6 and IL-1βwere respectively raised 3.4,3.7,4.7,3.7 times which were all significantly down-regulated after the import of *** detection results showed that compared with negative control group,the NGF content of cocultured medium treated by 10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/L I-L6 and IL-1βwere respectively raised 2.9,3.3,4.5,7.4 times which were all significantly decreased after the import of ***:These molecular biological results suggest that inflammatory factor IL-6 and IL-1βcould stimulate NCF on intervertebral disc cells in vitro culture model and its efficiency is concentration dependent,while siRNA interference can inhibit the stimulation effect of IL-6 and IL-1βon intervertebral disc cell,which provides a new targets for the clinical treatment of discogenic low back pain.

关键词： RNA small interfering Nerve growth factor Low back pain Intervertebral disk displacement

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Mechanism underlying the effect of Liujunzi decoction(六君子汤)on advanced-stage non-small cell lung cancer in patients after first-line chemotherapy

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Journal of Traditional Chinese Medicine 2022年第1期42卷 108-115页

作者： XIN Xiaoli WANG Guodong HAN Ru JIANG Yi LIU Chang LIU Lingshuang XU Zhenye Department of Oncology Longhua Hospital affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200032China Department of Orthopedics Longhua Hospital affiliated to Shanghai University of Traditional Chinese MedicineShanghai 20032China Department of human resources Shandong Academy of Occupational Health and Occupational MedicineJinan 250000China

OBJECTIVE:To further clarify the anticancer mechanisms of Liujunzi decoction(六君子汤)and provide possible targets for the treatment of advanced-stage nonsmall cell lung cancer(NSCLC)by re-analyzing differential gene expression profile of peripheral blood mononuclear cells(PBMCs)from Liujunzi decoctiontreated NSCLC patients receiving first-line ***:The PBMC gene expression microarray data set GSE61926 was retrieved from a high throughput gene expression *** expressed genes(DEGs)were screened by paired sample t-test and the multiple ratio *** ontology and Kyoto encyclopedia of genes and genomes(KEGG)pathway analyses were performed using the DAVID *** protein–protein interaction(PPI)network was constructed using interaction gene library retrieval tools and Cytoscape ***:A total of 162 DEGs were identified,with 67 upregulated genes and 95 downregulated *** functional distribution of Gene Oncology(GO)genes showed that DEGs were mostly concentrated in extracellular regions,calcium ion binding,and transcriptase *** pathway analysis showed that cytokine–cytokine receptor interactions were significantly *** network analysis screened out the top 10 central protein-coding genes with the highest nodal degree:IL2,PIWIL4,DICER1,PIWIL2,SAA1,XCL1,IL22RA1,ARHGAP11A,DCP1A,and *** them,the central protein-coding gene with the highest node degree was *** addition,the central protein-coding genes with high node degrees and high molecular complex detection(MCODE)scores were PIWIL4,DICER1,PIWIL2,and DCP1A,all of which are related to tumor ***:One signaling pathway and 10 central protein-coding genes related to anticancer mechanisms were screened by re-analysis of GSE61926 ***2,PIWIL4,DICER1,PIWIL2,and DCP1 A may have important roles in the mechanism of Liujunzi decoction treatment against *** results suggest that the anticancer mechanism of Liujunzi decoction ma

关键词： carcinoma non-small-cell lung antineoplastic agents computational biology RNA small interfering Liujunzi decoction

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