检索结果-南通市图书馆

Acta Pharmacologica Sinica 2004年第12期25卷 81-85页

作者： Hong XIE, Ke-ying CHEN, Pei-hong ZHU2 Unit of Cell Signal Transduction, Key Laboratory of Neuroscience, Shanghai Institutes for BiologicalSciences, Chinese Academy ofSciences, Shanghai200031, China 中科院上海生命科学院 Unit of Cell Signal Transduction Key Laboratory of Neuroscience CAS 上海 200031

AIM: To explore whether the differential effects of Zn2+ on ryanodine binding to the sarcoplasmic reticulum (SR) of skeletal and cardiac muscles resulted from different permeability of the SR to Zn2+. METHODS: [3H]ryanodine binding assays were performed to examine the effect of Zn2+ on ryanodine binding to the SR in the presence of pyrithione sodium (PyNa), a specific Zn2+ ionophore. RESULTS: As a control, PyNa up to 50 μmol/L did not induce any effect on ryanodine binding to the SR of cardiac muscle. But PyNa 1-100 μmol/L increased ryanodine binding in skeletal muscle with maximum binding (222.2 %±20.9 % of the control) and inhibited ryanodine binding to 50 % of the control at about 500 μmol/L. In the presence of PyNa 10 and 50 μmol/L the dose-dependence of the effect of Zn2+ in cardiac muscle was still monophasic and not changed by PyNa, while the biphasic effect of Zn2+ in skeletal muscle became monophasic. CONCLUSION: Different permeability of the SR to Zn2+ may account for the differential effects of Zn2+on ryanodine binding in skeletal and cardiac muscles. PyNa is not a strictly specific Zn2+ ionophore.

关键词： zinc ion pyrithione radioligand binding ryanodine receptor skeletal muscle myocardium

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor L.in growing pigs by transcriptomics, metabolomics and lipidomics

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2020年第2期11卷 527-544页

作者： Sandra Meyer Denise K.Gessner Maria S.Braune Theresa Friedhoff Erika Most Marcus Höring Gerhard Liebisch Holger Zorn Klaus Eder Robert Ringseis Institute of Animal Nutrition and Nutrition Physiology Justus Liebig University GiessenHeinrich-Buff-Ring 26-3235392 GiessenGiessenGermany Institute of Clinical Chemistry and Laboratory Medicine University Hospital of RegensburgFranz-Josef-Strauss-Allee 1193053 RegensburgGermany Institute of Food Chemistry and Food Biotechnology Justus Liebig University GiessenHeinrich-Buff-Ring 1735392 GiessenGiessenGermany Fraunhofer Institute for Molecular Biology and Applied Ecology Winchester Str.235394 GiessenGiessenGermany

Background:The hypothesis was tested that insect meal(IM)as protein source influences intermediary metabolism of growing pigs.To test this,30 male,5-week-old crossbred pigs were randomly assigned to 3 groups of 10 pigs each with similar body weights(BW)and fed isonitrogenous diets either without(CON)or with 5%IM(IM5)or 10%IM(IM10)from Tenebrio molitor L.for 4 weeks and key metabolic tissues(liver,muscle,plasma)were analyzed using omics-techniques.Results:Most performance parameters did not differ across the groups,whereas ileal digestibilities of most amino acids were 6.7 to 15.6%-units lower in IM10 than in CON(Pskeletal muscle revealed a total of 166 and 198,respectively,transcripts differentially expressed between IM10 and CON(P<0.05).Plasma metabolomics revealed higher concentrations of alanine,citrulline,glutamate,proline,serine,tyrosine and valine and a lower concentration of asparagine in IM10 than in CON(P<0.05).Only one out of fourteen quantifiable amino acid metabolites,namely methionine sulfoxide(MetS),in plasma was elevated by 45%and 71%in IM5 and IM10,respectively,compared to CON(P<0.05).Plasma concentrations of both,major carnitine/acylcarnitine species and bile acids were not different across groups.Lipidomics of liver and plasma demonstrated no differences in the concentrations of triacylglycerols,cholesterol and the main phospholipids,lysophospholipids and sphingolipids between groups.The percentages of all individual phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species in the liver showed no differences between groups,except those with 6 double bonds(PC 38:6,PC 40:6,PE 38:6,PE 40:6),which were markedly lower in IM10 than in CON(P<0.05).In line with this,the percentage of C22:6n-3 in hepatic total lipids was lower in IM10 than in the other groups(P<0.05).Conclusions:Comprehensive analyzes of the transcriptome,lipidome and metabolome of key metabolic tissues indicate that partial or complete replacement of a conventional protein source by IM in the die

关键词： Animal nutrition Insect meal Lipidome Liver Metabolism Metabolome Pigs Plasma skeletal muscle Transcriptome

Protective effects of taurine against muscle damage induced by diquat in 35 days weaned piglets

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2020年第4期11卷 1175-1188页

作者： Chaoyue Wen Fengna Li Qiuping Guo Lingyu Zhang Yehui Duan Wenlong Wang Jianzhong Li Shanping He Wen Chen Yulong Yin Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process Changsha 410125China Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production Changsha 410125China National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Changsha 410125China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central Ministry of AgricultureChangsha 410125China Laboratory of Animal Nutrition and Human Health Hunan Normal UniversityChangsha 410081HunanChina Hunan International Joint Laboratory of Animal Intestinal Ecology and Health Hunan Normal UniversityChangsha 410081HunanChina Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation College of Life ScienceHunan Normal UniversityChangsha 410081HunanChina University of Chinese Academy of Sciences Beijing 100039China.

Background: Oxidative stress is a key factor that influences piglets' health. Taurine plays an imperative role in keeping the biological system from damage. This study was conducted to investigate the protective effect of taurine against muscle injury due to the secondary effect of diquat toxicity.Results: Our study found that taurine effectively and dose-dependently alleviated the diquat toxicity induced rise of feed/gain, with a concurrent improvement of carcass lean percentage. The plasma content of taurine was considerably increased in a dose-dependent manner. Consequently, dietary taurine efficiently improved the activity of plasma antioxidant enzymes. Furthermore, taurine attenuated muscle damage by restoring mitochondrial micromorphology, suppressing protein degradation and reducing the percentage of apoptotic cells in the skeletal muscle. Taurine supplementation also suppressed the genes expression levels of the antioxidant-, mitochondrial biogenesis-, and muscle atrophy-related genes in the skeletal muscle of piglets with oxidative stress.Conclusions: These results showed that the dose of 0.60% taurine supplementation in the diet could attenuate skeletal muscle injury induced by diquat toxicity. It is suggested that taurine could be a potential nutritional intervention strategy to improve growth performance.

关键词： Mitochondrial morphology Oxidative stress Piglets skeletal muscle Taurine

The role of mitochondria in redox signaling of muscle homeostasis

在线全文

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2020年第5期9卷 386-393页

作者： Li Li Ji Dongwook Yeo Chounghun Kang Tianou Zhang The Laboratory of Physiological Hygiene and Exercise Science University of Minnesota Twin CitiesMinneapolisMN 55455USA Department of Orthopedic Surgery Mayo ClinicRochesterMN 55905USA Department of Physical Education Inha UniversityIncheon 22212Republic of Korea Department of Kinesiology Health and NutritionUniversity of Texas at San AntonioSan AntonioTX 78249USA

In the past,contraction-induced production of reactive oxygen species(ROS)has been implicated in oxidative stress to skeletal muscle.As research advances,clear evidence has revealed a more complete role of ROS under both physiologic and pathologic conditions.Central to the role of ROS is the redox signaling pathways that control exercise-induced major physiologic and cellular responses and adaptations,such as mitochondrial biogenesis,mitophagy,mitochondrial morphologic dynamics,antioxidant defense,and inflammation.The current review focuses on how muscle contraction and immobilization may activate or inhibit redox signalings and their impact on muscle mitochondrial homeostasis and physiologic implications.

关键词： Antioxidant Exercise Peroxisome-proliferator-activated receptorγcoactivator 1-α Reactive oxygen species Redox signaling skeletal muscle

The SWI/SNF chromatin-remodeling factors BAF60a, b, and c in nutrient signaling and metabolic control

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2018年第2期9卷 207-215页

作者： Ruo-Ran Wang Ran Pan Wenjing Zhang Junfen Fu Jiandie D. Lin Zhuo-Xian Meng Department of Pathology and Pathophysiology Key Laboratory of Disease Proteomics of Zhejiang Province School of Medicine Chronic Disease Research Institute of School of Public Health Zhejiang University Hangzhou 310058 China Children's Hospital Zhejiang University School of Medicine Hangzhou 310003 China Life Sciences Institute and Department of Cell & Developmental Biology University of Michigan Medical Center Ann Arbor MI 48109 USA

Metabolic syndrome has become a global epidemic that adversely affects human health. Both genetic and environmental factors contribute to the pathogenesis of metabolic disorders; however, the mechanisms that integrate these cues to regulate metabolic physiology and the development of metabolic disorders remain incompletely defined. Emerging evidence suggests that SWlISNF chromatin.remodeling complexes are critical for directing metabolic reprogramming and adaptation in response to nutritional and other physiological sigrials. The ATP-dependent SWl/SNF ing complexes comprise up to 11 subunits, among which the BAF60 subunit serves as a key link between the core complexes and specific transcriptional factors. The BAF60 subunit has three members, BAF60a, b, and c. The distinct tissue distribution patterns and regulatory mechanisms of BAF60 proteins confer each isoform with specialized functions in different m^abolic cell types. In this review, we summarize the emerging roles and mechanisms of BAF60 proteins in the regulation of nutrient sensing and energy metabolism under physiological and disease conditions.

关键词： BAF60a BAF60b BAF60c chromatin- remodeling SWI/SNF energy metabolism nutrient sensing glucose lipid skeletal muscle liver

The mRNA Binding Proteome of Proliferating and Differentiated muscle Cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2020年第4期18卷 384-396页

作者： Monika Hiller Miriam Geissler George Janssen Peter van Veelen Annemieke Aartsma-Rus Pietro Spitali Department of Human Genetics Leiden University Medical CenterLeiden 2333 ZCthe Netherlands Center for Proteomics and Metabolomics Leiden University Medical CenterLeiden 2333 ZCthe Netherlands

muscle formation is a coordinated process driven by extensive gene expression changes where single cells fuse together to form multinucleated muscle fibers. Newly synthesized m RNAs are then regulated by RNA binding proteins(RBPs), affecting post-transcriptional transcript metabolism. Here, we determined how large-scale gene expression changes affect the catalog of RBPs by studying proliferating and differentiated muscle cells in healthy and dystrophic conditions. Transcriptomic analysis showed that the expression of more than 7000 genes was affected during myogenesis. We identified 769 RBPs, of which 294 were muscle-specific and 49 were uniquely shared with cardiomyocytes. A subset of 32 RBPs(half of which were muscle-specific) was found to be preferentially associated with target mRNAs in either myoblasts(MBs) or myotubes(MTs). A large proportion of catalytic proteins were bound to mRNAs even though they lack classical RNA binding domains. Finally, we showed how the identification of cell-specific RBPs enabled the identification of biomarkers that can separate healthy individuals from dystrophic patients. Our data show how interactome data can shed light on new basic RNA biology as well as provide cell-specific data that can be used for diagnostic purposes.

关键词： RNA binding protein Interactome Duchenne muscular dystrophy Proteomics skeletal muscle

Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2021年第4期12卷 1662-1674页

作者： Jinsol Jeong Kwang-Hwan Choi Seung-Hun Kim Dong-Kyung Lee Jong-Nam Oh Mingyun Lee Gyung Cheol Choe Chang-Kyu Lee Department of Agricultural Biotechnology Animal Biotechnology Majorand Research Institute of Agriculture and Life ScienceSeoul National UniversitySeoul 08826South Korea Present address:Research and Development Center Space F corporationHwasung-siGyeonggi-do 18471South Korea Institute of Green Bio Science and Technology Seoul National UniversityPyeong ChangKangwon-do 25354South Korea

Background:Myogenic transdifferentiation can be accomplished through ectopic MYOD1 expression,which is facilitated by various signaling pathways associated with myogenesis.In this study,we attempted to transdifferentiate pig embryonic fibroblasts(PEFs)myogenically into skeletal muscle through overexpression of the pig MYOD1 gene and modulation of the FGF,TGF-β,WNT,and cAMP signaling pathways.Results:The MYOD1 overexpression vector was constructed based on comparative sequence analysis,demonstrating that pig MYOD1 has evolutionarily conserved domains across various species.Although forced MYOD1 expression through these vectors triggered the expression of endogenous muscle markers,transdifferentiated muscle cells from fibroblasts were not observed.Therefore,various signaling molecules,including FGF2,SB431542,CHIR99021,and forskolin,along with MYOD1 overexpression were applied to enhance the myogenic reprogramming.The modified conditions led to the derivation of myotubes and activation of muscle markers in PEFs,as determined by qPCR and immunostaining.Notably,a sarcomere-like structure was observed,indicating that terminally differentiated skeletal muscle could be obtained from transdifferentiated cells.Conclusions:In summary,we established a protocol for reprogramming MYOD1-overexpressing PEFs into the mature skeletal muscle using signaling molecules.Our myogenic reprogramming can be used as a cell source for muscle disease models in regenerative medicine and the production of cultured meat in cellular agriculture.

关键词： MYOD1 Pig Sequence analysis skeletal muscle Transdifferentiation

Defective maintenance of intracellular Ca2+ homeostasis is linked to increased muscle fatigability in the MG29 null mice

在线全文

学校读者我要写书评

暂无评论

Cell Research 2004年第5期14卷 373-378页

作者： Marco A. P. BROTTO Ramakrishnan Y. NAGARAJ Leticia S. BROTTO Thomas M. NOSEK Department of Physiology and Biophysics UMDNJ-Robert Wood Johnson Medical School Piscataway NJ 08854 USA School of Medicine Case Western Reserve University Cleveland OH 44106

Mitsugumin 29 (MG29) is a transmembrane protein that is normally found in the triad junction of skeletal muscle. Our previous studies have shown that targeted deletion of mg29 from the skeletal muscle resulted in abnormality of the triad junction structure, and also increased susceptibility to muscle fatigue. To elucidate the basis of these effects, we investigated the properties of Ca2+-uptake and -release in toxin-skinned Extensor Digitorium Longus (EDL) muscle fibers from control and mg29 knockout mice. Compared with the control muscle, submaximal Ca2+-uptake into the sarcoplasmic reticulum (SR) was slower and the storage of Ca2+ inside the SR was less in the mutant muscle, due to increased leakage process of Ca2+ movement across the SR. The leakage pathway is associated with the increased sensitivity of Ca2+/caffeine -induced Ca2+ release to myoplasmic Ca2+. Therefore, the increased fatigability of mutant EDL muscles can result from a combination of a slowing of Ca2+ uptake, modification of Ca2+-induced Ca2+ release (CICR), and a reduction in total SR Ca2+ content.

关键词： MG29 mutant skeletal muscle skinned fibers ECC Ca2+ uptake CICR.

Global transcriptomic analysis reveals Lnc-ADAMTS9 exerting an essential role in myogenesis through modulating the ERK signaling pathway

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2021年第2期12卷 520-533页

作者： Liqi Wang Ting He Xin Zhang Yubo Wang Kai Qiu Ning Jiao Linjuan He Jingdong Yin State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityNo.2 Yuanmingyuan West RoadBeijing 100193China

Background:Long non-coding RNAs(lncRNAs)are emerging key regulators involved in a variety of biological processes such as cell differentiation and development.The balance between myogenesis and adipogenesis is crucial for skeletal muscle homeostasis in humans and meat quality in farm animals.The present study aimed to reveal the global transcriptomic profiles of adipogenic(Adi-)and myogenic(Myo-)precursors derived from porcine skeletal muscle and identify lncRNAs involved in the modulation of myogenesis homeostasis in porcine skeletal muscle.Results:In this study,a total of 655 novel individual lncRNAs including differentially expressed 24 lncRNAs,and 755 differentially expressed mRNAs were identified(fold change≥2 or≤0.5 and adjusted Pskeletal muscle.Although Lnc-ADAMTS9 knock-down did not alter the mRNA expression of ADAMTS9,we demonstrated that Lnc-ADAMTS9 can promote myogenic proliferation and myogenic differentiation of myogenic precursors through inhibiting the ERK/MAPK signaling pathway.Conclusion:We deciphered a comprehensive catalog of mRNAs and lncRNAs that might be involved in the regulation of myogenesis and adipogenesis homeostasis in the skeletal muscle of pigs.The Lnc-ADAMTS9 exerts an essential role in myogenesis through the ERK signaling pathway.

关键词： Adipogenic precursors LncRNA Myogenic differentiation Myogenic precursors Myogenic proliferation Pigs skeletal muscle

Transferrin receptor 1 plays an important role in muscle development and denervation-induced muscular atrophy

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第7期16卷 1308-1316页

作者： Ying Li Juan-Xian Cheng Hai-Hong Yang Li-Ping Chen Feng-Jiao Liu Yan Wu Ming Fan Hai-Tao Wu Department of Neurobiology Beijing Institute of Basic Medical SciencesBeijingChina Department of Anesthesiology the General Hospital of Western Theater CommandChengduSichuan ProvinceChina Chinese Institute for Brain Research(CIBR) BeijingChina Key Laboratory of Neuroregeneration Coinnovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina

Previous studies demonstrate an accumulation of transferrin and transferrin receptor 1(TfR1) in regenerating peripheral nerves.However, the expression and function of transferrin and TfR1 in the denervated skeletal muscle remain poorly understood.In this study, a mouse model of denervation was produced by complete tear of the left brachial plexus nerve.RNA-sequencing revealed that transferrin expression in the denervated skeletal muscle was upregulated, while TfR1 expression was downregulated.We also investigated the function of TfR1 during development and in adult skeletal muscles in mice with inducible deletion or loss of TfR1.The ablation of TfR1 in skeletal muscle in early development caused severe muscular atrophy and early death.In comparison, deletion of TfR1 in adult skeletal muscles did not affect survival or glucose metabolism, but caused skeletal muscle atrophy and motor functional impairment, similar to the muscular atrophy phenotype observed after denervation.These findings suggest that TfR1 plays an important role in muscle development and denervation-induced muscular atrophy.This study was approved by the Institutional Animal Care and Use Committee of Beijing Institute of Basic Medical Sciences, China(approval No.SYXK 2017-C023) on June 1, 2018.

关键词： brachial plexus nerve innervation iron motor dysfunction muscle atrophy signal skeletal muscle transferrin