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Chinese Chemical Letters 2022年第2期33卷 751-756页

作者： Zhihong Wei Boyang Wang Mingcai Xie Daocheng Hong Xin Yang Sushu Wan Weiqing Yang Siyu Lu Yuxi Tian Key Laboratory of Mesoscopic Chemistry of MOE School of Chemistry and Chemical EngineeringJiangsu Key Laboratory of Vehicle Emissions ControlNanjing UniversityNanjing 210023China Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou 450001China Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province Yancheng Institute of TechnologyYancheng 224051China

Carbon dots(CDs), because of their unique properties, are being rapidly developed as important luminescent materials for imaging, sensing, and use in photonic devices. However, most of the reported fundamental properties of the CDs are results of investigations conducted in the solution state, which may be completely different from those conducted in the solid state. In this work, we study the luminescence properties, photostability, and the dynamics of CDs in different matrix environments, from ensemble to the single-particle level. We observed that the properties associated with the emission centers and photostability of CDs were extremely sensitive to the local chemical environment. A better understanding of the dependence of the spectroscopic properties of CDs on the complex local chemical environment is an important step toward finding new ways of controlling the optical properties of CDs and optimizing their use in various applications.

关键词： Carbon dots Matrix effects single-particle Photostability Blinking

Dual Rolling Circle Amplification- Assisted single-particle Fluorescence Profiling of Exosome Heterogeneity for Discriminating Lung Adenocarcinoma from Pulmonary Nodules

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CCS Chemistry 2023年第4期5卷 947-957页

作者： Yan Zhou Haoxiang Li Min Hou Jianjun He Jian-Hui Jiang Hunan Key Laboratory of Organ Fibrosis Department of Pulmonary and Critical Care MedicineThe Third Xiangya HospitalCentral South UniversityChangsha 410013 State Key Laboratory of Chemo/BioSensing and Chemometrics College of Biomedical SciencesCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082

Exosomes secreted by tumor cells carry abundant molecular biomarkers that reflect the status of their originating cells.These tumor-derived exosomes(TDEs)have emerged as attractive diagnostic targets.However,the identification and characterization of highly heterogeneous TDEs remain practically challenging.Here,we report a dual rolling circle amplification(DRCA)-assisted approach for the selective encapsulation of single TDEs for fluorescence microscopic and flow cytometric analysis.TDEs have been targeted by aptamers that recognized their surface tumor marker and exosomal marker CD63,following DRCA that produced entangling polymeric DNA chains,resulting in facile particle enlargement that allows single-particle fluorescence profiling of exosome heterogeneity.We have demonstrated the use of a dual-marker positive ratio for exosome differentiation and applied division and multiplication operations for normalized andmagnified marker heterogeneity analysis.We further applied this assay to distinguish lung adenocarcinoma and pulmonary nodule patients and found an accuracy of 90%.We anticipate promising transformations of this straightforward assay into clinically implantable diagnostic methods.

关键词： rolling circle amplification exosomes single-particle fluorescence analysis cancer diagnosis

single-particle Tracking for the Quantification of Membrane Protein Dynamics in Living Plant Cells

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Molecular Plant 2018年第11期11卷 1315-1327页

作者： Yaning Cui Meng Yu Xiaomin Yao Jingjing Xing Jinxing Lin Xiaojuan Li Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry UniversityBeijing 100083China College of Biological Sciences &Biotechnology Beijing Forestry UniversityBeijing 100083China Institute of Plant Stress Biology State Key Laboratory of Cotton BiologyDepartment of BiologyHenan UniversityJinming StreetKaifeng 475001China

The plasma membrane is a sophisticated,organized,and highly heterogeneous structure that compartmentalizes cellular processes.To decipher the biological processes involving membrane proteins,it is necessary to analyze their spatiotemporal dynamics.However,it is difficult to directly assess the dynamics and interactions of biomolecules in living cells using traditional biochemical methods.singleparticle tracking (SPT)methods for imaging and tracking single particles conjugated with fluorescent probes offer an ideal approach to acquire valuable and complementary information about dynamic intracellular processes.SPT can be used to quantitatively monitor the diverse motions of individual particles in living cells.SPT also provides super-spatiotemporal resolution that allows early-stage or rapid response information to be obtained for a better understanding of molecular basis of associated signal transduction processes.More importantly,SPT can be used to detect the motion paths of individual biomolecules in vivo and in situ,thus unveiling the dynamic behavior of the biomolecules that support developmental processes in living cells.In this review,we give an overview of SPT methods,from image acquisition to the detection of single particles,as well as tracking and data analysis.We also discuss recent applications of SPT methods in the field ofplant biology to reveal the complex biological functions of membrane proteins.

关键词： single-particle tracking dynamics stoichiometry dwell time colocalization

Error analysis in correlation computation of single particle reconstruction technique

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Science China(Technological Sciences) 1999年第3期42卷 260-265页

作者：胡悦隋森芳 Department of Biological Sciences and Biotechnology Tsinghua University Beijing China

The single particle reconstruction technique has become particularly important in the structure analysis of hiomaeromolecules. The problem of reconstructing a picture from identical samples polluted by colored noises is studied, and the alignment error in the correlation computation of single particle reconstruction technique is analyzed systematically. The concept of systematic error is introduced, and the explicit form of the systematic error is given under the weak noise approximation. The influence of the systematic error on the reconstructed picture is discussed also, and an analytical formula for correcting the distortion in the picture reconstruction is obtained.

关键词： single-particle reconstruction correlation function alignment colored noise.

single-particle resonances in a deformed relativistic potential

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Science China(Physics,Mechanics & Astronomy) 2010年第4期53卷 773-778页

作者： VRETENAR Dario Physics Department Faculty of Science University of Zagreb Croatia

The positive-parity single-neutron levels in an axially-deformed relativistic quadrupole Woods-Saxon potential are analyzed. Neutron states are obtained as the solutions of the corresponding single-particle Dirac equation, using the coupled-channels method in the coordinate space. The evolution of the levels close to the continuum threshold and, in particular, the occurrence of singleneutron resonant states as the functions of the axial deformation parameter 0 β 0.5, are examined using the eigenphase representation. Calculations are performed for different values of the radius of the potential (R/r 0 ) 3 , corresponding to a variation of the mass number A.

关键词： single-particle resonance Dirac equation quadrupole deformation coupled-channels method

Rapid ultrasensitive monitoring the single-particle surface-enhanced Raman scattering (SERS) using a dark-field microspectroscopy assisted system

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中国化学快报：英文版 2020年第2期31卷 473-475页

作者： Shaochuang Liu Yilun Ying Yitao Long School of Chemistry and Molecular Engineering East China University of Science and TechnologyShanghai 200237China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

The observation of single-particle surface-enhanced Raman scattering(SERS) has generated considerable interest both in the nanomaterials filed and in the single-particle spectroscopy community.It is a challenge to realize rapid,facile,and high throughput SERS at single nanoparticle level.Here,without the complex experimental device and difficult experimental operations,a general single-particle SERS technique has been achieved by using dark-field-assisted surface-enhanced Raman spectroscopy(DFSERS).This advanced method provides in-situ characterization of the chemical reaction performance at single gold nanorod.

关键词： SERS Dark-field microscope single-particle detection Plasmon resonance scattering Gold nanorod Chemical reaction monitoring

The Experiment of Obtaining Micron Beam in the single-particle Microbeam Facility of the Institute of Plasma Physics

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Plasma Science and Technology 2003年第4期5卷 1927-1931页

作者：吴瑜李军陈斌胡素华张俊王绍虎余增亮 Institute of Plasma Physics Chinese Academy of SciencesHefei 230031China Institute of Plasma Physics Chinese Acadeny of SciencesHefei 230031China

The Experiments, methods and results of obtaining micron beam in the Microbeam Facility of the Institute of Plasma Physics were discussed in this paper. The H+2 beam was accelerated by the Van de GraafF electrostatic accelerator, and the collimator at the end of the beam line is a 60 μm thick stainless steel chip. And as a result, particle tracks on the solid track probes (CR39 film) etched in the solution of NaOH showed that the beam can go through the collimator with a small aperure (2000, 300, 55, 30, or 10 μm) and 3.5 μm thick vacuum film (Mylar). Besides the CR39 method, the beam was measured by an energy spectrum detector after the 10 μm diameter aperture and the 3.5 μm thick vacuum film too.

关键词： single-particle microbeam facility micron beam

Interaction of silver nanoparticles with mediterranean agricultural soils: Lab-controlled adsorption and desorption studies

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Journal of Environmental Sciences 2019年第9期31卷 205-216页

作者： Laura Torrent Eva Margui Ignasi Queralt Manuela Hidalgo Monica Iglesias Department of Chemistry University of Girona C/M. Aurelia Capmany 69 17003 Girona Spain Institute of Environmental Assessment and Water Research (IDAEA-CSIC) C. Jordi Girona 28-26 08034 Barcelona Spain

The production of silver nanoparticles(AgNPs) has increased tremendously during recent years due to their antibacterial and physicochemical properties. As a consequence, these particles are released inevitably into the environment, with soil being the main sink of disposal. Soil interactions have an effect on AgNP mobility, transport and bioavailability. To understand AgNP adsorption processes, lab-controlled kinetic studies were performed.Batch tests performed with five different Mediterranean agricultural soils showed that cation exchange capacity and electrical conductivity are the main parameters controlling the adsorption processes. The adsorption kinetics of different sized(40, 75, 100 and 200 nm)and coated(citrate, polyvinylpyrrolidone and polyethyleneglycol(PEG)) AgNPs indicated that these nanoparticle properties have also an effect on the adsorption processes.To assess the mobility and bioavailability of AgNPs and to determine if their form is maintained during adsorption/desorption processes, loaded soils were submitted to leaching tests three weeks after batch adsorption studies. The DIN 38414-S4 extraction method indicated that AgNPs were strongly retained on soils, and single-particle inductively coupled plasma mass spectrometry confirmed that silver particles maintained their nanoform, except for 100 nm PEG-AgNPs and 40 nm citrate-coated AgNPs. The DTPA(diethylenetriaminepentaacetic acid) leaching test was more effective in extracting silver,but there was no presence of AgNPs in almost all of these leachates.

关键词： Silver nanoparticles Agricultural soils Sorption kinetics Leaching tests single-particle inductively coupled plasma mass spectrometry

The Progress of a Microbeam Facility in the Institute of Plasma Physics

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Plasma Science and Technology 2003年第2期5卷 1761-1764页

作者：吴瑜张束清袁航余增亮张俊胡素华李军王旭飞陈斌时钟涛王绍虎 Institute of Plasma Physics Chinese Academy of Sciences

The progress of a microbeam facility in the Institute of Plasma Physics was discussed in this paper. This kind of equipment can supply single-particle beam which may be implanted into cells in micrometer-radius and measured by a new outstanding detector among global microbeam systems. Measurements by some plain targets showed that the highest current after the accelerator tube can be larger than 20 /μA, the H_2^+ current before the second bending magnet is near 0.9 /μA, the current after the second bending magnet is near 0.8 μA, and the current of the beam line (after a 2-mm diameter aperture) is near 0.25 nA which is enough for the single-particle microbeam experiment. It took scientists 3 months to do their microbeam experiment after setting up the accelerator beam line and get the microbeam from this equipment. Two pre-collimators were installed between the 2-mm diameter aperture and the collimator to survey the beam. Tracks on the CR39 film etched in the solution of NaOH showed that the beam can go through the collimator including a 10 μm diameter aperture and the 3.5 μm thick vacuum sealing film (Mylar). A new method, which is called optimization of the beam quality, was put forward in this paper, in order to get smaller diameter of beam-spot in microbeam system.

关键词： single-particle microbeam beam spot pre-detection

Fringe visibility for two qubits interacting with a macroscopic medium

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Communications in Theoretical Physics 2020年第4期72卷 37-44页

作者：李玉恒刘彦军卢竞周兰 Department of Physics and Key Laboratory for Matter Microstructure and Function of Hunan Province Hunan Normal UniversityChangsha 410081China College of Physics Mechanical and Electronical CollegeShijiazhuang UniversityShijiazhuang050035HebeiChina

We study both the two-particle and single-particle fringe visibility in the generalized version of the Nakazato–Pascazio model where two qubits interact with a finite length one-dimensional array.Both the two-particle and single-particle fringe visibilities are investigated with different initial states of the particles spin.For different initial states of the particles spin,the two-particle fringe visibility either decreases or increases over time,and may even decrease first and increase later.Due to the interaction between the particles and the one-dimensional array,the single-particle fringe visibility increases over time when the initial state of the two particles spin is independent.The single-particle fringe visibility is equal to 0 as the two-particle spin is initially in the completely entangled state or in the singlet state.

关键词： two-particle fringe visibility single-particle fringe visibility Nakazato-Pascazio generalized model