检索结果-南通市图书馆

On-chip detection of a single nucleotide polymorphism without polymerase amplification

Nano Research 2014年第9期7卷 1302-1310页

作者： Jinhee Han Matthew Tan Lakshmana Sudheendra Robert H. Weiss Ian M. Kennedy Department of Mechanical and Aerospace Engineering University of California Davis California 95616 USA Division of Nephrology Department of Internal Medicine University of California Davis California 95616 USA Medical Service Sacramento VA Medical Center Sacramento California 95655 USA

A nanoparticle-assembled photonic crystal （PC） array was used to detect single nucleotide polymorphism （SNP）. The assay platform with PC nanostructure enhanced the fluorescent signal from nanoparticle-hybridized DNA complexes due to phase matching of excitation and emission. Nanoparticles coupled with probe DNA were trapped into nanowells in an array by using an electrophoretic particle entrapment system. The PC/DNA assay platform was able to identify a 1 base pair （bp） difference in synthesized nucleotide sequences that mimicked the mutation seen in a feline model of human autosomal dominant polycystic kidney disease （PKD） with a sensitivity of 0.9 fg/mL （50 aM）-sensitivity, which corresponds to 30 oligos/array. The reliability of the PC/DNA assay platform to detect SNP in a real sample was demonstrated by using genomic DNA （gDNA） extracted from the urine and blood of two PKD-wild type and three PKD positive cats. The standard curves for PKD positive （PKD＋） and negative （PKD-） DNA were created using two feline-urine samples. An additional three urine samples were analyzed in a similar fashion and showed satisfactory agreement with the standard curve, confirming the presence of the mutation in affected urine. The limit of detection （LOD） was 0.005 ng/mL which corresponds to 6 fg per array for gDNA in urine and blood. The PC system demonstrated the ability to detect a number of genome equivalents for the PKD SNP that was very similar to the results reported with real time polymerase chain reaction （PCR）. The favorable comparison with quantitative PCR suggests that the PC technology may find application well beyond the detection of the PKD SNP, into areas where a simple, cheap and portable nucleic acid analvsis is desirable.

关键词： photonic crystal array single nucleotidepolymorphisms DNA polycystic kidney disease real time polymerasechain reaction (PCR)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Breastfeeding and genetic factors in the etiology of inflammatory bowel disease in children

引用

World Journal of Gastroenterology 2009年第3期15卷 270-279页

作者： Theresa A Mikhailov Sylvia E Furner Department of Pediatrics Division of Critical CareMedical College of Wisconsin9000 W.Wisconsin AvenueMS# 681MilwaukeeWI 53226United States School of Public Health Division of Epidemiology and BiostatisticsUniversity of Illinois Chicago 1603 W.Taylor St.M/C 923ChicagoIL 60612United States

Inflammatory bowel disease is a chronic,debilitating disorder of the gastrointestinal *** etiology of inflammatory bowel disease has not been elucidated,but is thought to be multifactorial with both environmental and genetic influences.A large body of research has been conducted to elucidate the etiology of inflammatory bowel *** article reviews this literature,emphasizing the studies of breastfeeding and the studies of genetic factors,particularly NOD2 polymorphisms.

关键词： Inflammatory bowel disease Crohn'sdisease Ulcerative colitis Etiology Risk factors Protective factors NOD2/CARD15 single nucleotidepolymorphisms

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inherited susceptibility for aggressive prostate cancer

引用

Asian Journal of Andrology 2012年第3期14卷 415-418,I0006页

作者： William B Isaacs The Brady Urological Institute Johns Hopkins Medical institutions Baltimore MD 21287 USA

Whether or not there is inherited basis for prostate cancer aggressiveness is not clear, but advances in DNA analysis should provide an answer to this question in the very near future.

关键词： aggressiveness germline Genome Wide Association Studies GWAS inherited linkage mutation single nucleotidepolymorphisms S N Ps susceptibility

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The association of lumican polymorphisms and high myopia in a Southern Chinese population

引用

International Journal of Ophthalmology(English edition) 2017年第10期10卷 1516-1520页

作者： Gui-Fang Wang Qing-Shan Ji Bing Qi Guo-Cheng Yu Lian Liu Jing-Xiang Zhong Department of Ophthalmology Loudi Central Hospital of Hunan Department of Ophthalmology the First Affiliated Hospital of Jinan University Department of Ophthalmology the Affiliated Anhui Provincial Hospital of Anhui Medical University

AIM：To investigate the correlation between lumican（LUM）gene and high myopia in a Southern Chinese ***：The study comprised of 95 high myopia patients with a spherical equivalent≤-6.5 diopters（D）.The control group recruited 95 individuals with a spherical equivalent ranging from-0.5 D to＋0.5 *** sequencing was used to detect the single nucleotide polymorphisms（SNPs）of LUM gene in coding *** distributions were tested for Hardy-Weinberg *** and allelic frequencies were analyzed through Chi-square test or Fisher’s exact ***：We identified 3 SNPs of the LUM gene：LUM c.32（rs577456426）,LUM c.507（rs17853500）and LUM c.849（rs181915277）.Among the three SNPs,the genotype and allele frequencies of rs17853500 showed a significant difference between patients and control subjects（P〈0.05）.However,there were no significant differences in rs181915277and rs577456426 between the two groups（P〉0.05）.CONCLUSION：LUM c.507 polymorphism may be a risk factor for the pathogenesis of high myopia in the Southern Chinese population.

关键词： lumican sclem high myopia single nucleotidepolymorphisms

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：