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Study on the simultaneously Quantitative Detection for β-Lactoglobulin and Lactoferrin of Cow Milk by Using Protein Chip Technique

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Biomedical and Environmental Sciences 2017年第12期30卷 875-886页

作者： YIN Ji Yong HUO Jun Sheng MA Xin Xin SUN Jing HUANG Jian National Institute for Nutrition and Health Chinese Center for Disease Control and Prevention Beijing 100050China

Objective To research a protein chip method which can simultaneously quantitative detect β-Lactoglobulin （β-L） and Lactoferrin （Lf） at one time. Methods Protein chip printer was used to print both anti-β-L antibodies and anti-Lf antibodies on each block of protein chip. And then an improved sandwich detection method was applied while the other two detecting antibodies for the two antigens were added in the block after they were mixed. The detection conditions of the quantitative detection for simultaneous measurement of β-L and Lf with protein chip were optimized and evaluated. Based on these detected conditions, two standard curves of the two proteins were simultaneously established on one protein chip. Finally, the new detection method was evaluated by using the analysis of precision and accuracy. Results By comparison experiment, mouse monoclonal antibodies of the two antigens were chosen as the printing probe. The concentrations of β-L and Lf probes were 0.5 mg/mL and 0.5 mg/mL, respectively, while the titers of detection antibodies both of β-L and Lf were 1：2,000. Intra- and inter-assay variability was between 4.88% and 38.33% for all tests. The regression coefficients of protein chip comparing with ELISA for β-L and Lf were better than 0.734, and both of the two regression coefficients were statistically significant （r = 0.734, t = 2.644, P = 0.038; and r = 0.774, t = 2.998, P = 0.024）. Conclusion A protein chip method of simultaneously quantitative detection for β-L and Lf has been established and this method is worthy in further application.

关键词： Protein chip simultaneously Quantitative detection β-Lactoglobulin Lactoferrin

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维普期刊数据库

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A multifunctional integrated simultaneously online screening microfluidic biochip for the examination of “efficacy-toxicity” and compatibility of medicine

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Chinese Chemical Letters 2019年第2期30卷 403-405页

作者： Hechen Wang Tianjiao Li Yongrui Bao Shuai Wang Xiansheng Meng College of Liaoning University of Traditional Chinese Medicine Liaoning University of Traditional Chinese Medicine-Agilent Technologies Modern TCM and Multi-Omics Research Collaboration Lab Liaoning University of Traditional Chinese Medicine

A multifunctional integrated microfluidic biochip device was engineered to estimate the activity-toxicity and composition principle of medicine in a cell model in vitro. This biochip could be used for disease cells and healthy cells in two modules of "Yin-Yang" on the same chip for detecting the medicine efficacytoxicity simultaneously, as well as adjust different gradient ratios of concentration through the Christmas tree structure in both "Yin-Yang" modules autonomously for detecting the best compatibility of medicine in maximum efficacy and minimal toxicity. In the applicability experiment, the best concentration of three chemical compounds including dinatin, diosmetin and cisplatin, were detected using the biochip and traditional 96-cell plate. Biochip assays showed perfect positive correlation compared with the results of traditional 96-cell plate, in addition presented advantages as less detection time and much lower price than the traditional 96-cell plate, which indicated the biochip is both convenient and ***, the novel microfluidic chip-based multifunctional integrated system congregated the virtues of high throughput, rapid, sensitive, specific, cost-effective, and similar to the physical environment of the human body, which was especially suitable for the medicine efficacy-toxicity and compatibility evaluation.

关键词： Multifunctional integrated microfluidic biochip Cirsium setosum(Wild.) Efficacy-toxicity of medicines Compatibility of medicines simultaneously online screening

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Method for simultaneously and directly measuring all six-DOF motion errors of a rotary axis

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Chinese Optics Letters 2019年第1期17卷 47-51页

作者： Jiakun Li Qibo Feng Chuanchen Bao Bin Zhang Key Laboratory of Luminescence and Optical Information Ministry of Education Beijing Jiaotong University

We report a method for simultaneously and directly measuring all six-degrees-of-freedom(six-DOF) motion errors of a rotary axis. Such a method combines the principles of laser interferometry and laser collimation measurement. One reference rotary axis and two retro-reflectors are used to achieve simultaneous sensitivity to all six errors in a full-circle measuring range. As no separation models are required, our method is capable of dynamically measuring these errors in real time and conveniently determining the origin of the errors. An automatic measuring device is built. The effectiveness of our method is experimentally demonstrated.

关键词： simultaneously directly measuring six-DOF motion errors

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Simultaneous Identification of Vehicular Parameters and Structural Damages in Bridge

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Wuhan University Journal of Natural Sciences 2018年第1期23卷 84-92页

作者： ZHANG Xiaozhong SUN Guomin SUN Yanhua ZHAO Zhifeng School of Civil and Architectural Engineering Guizhou University of Engineering Science

In this paper, we present a method for simultaneously identifying the vehicular parameters and the structural damage of bridges. By using the dynamic response data of bridge in coupled vibration state and the algorithm for the inverse problem, the vehicle-bridge coupling model is built through combining the motion equations of both vehicle and the bridge based on their interaction force relationship at contact point. Load shape function method and Newmark iterative method are used to solve the vibration response of the coupled system. Penalty function method and regularization method are interchangeable in the process until the error is less than the allowable value. The proposed method is applied on a single-span girders bridge, and the recognition results verify the feasibility, high accuracy and robustness of the method.

关键词： vehicle-bridge coupling model vehicular parameters structural damage in bridges identification method simultaneously recognition accuracy better robustness

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Construction and optimization of a base editor based on the MS2 system

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Animal Models and Experimental Medicine 2019年第3期2卷 185-190页

作者： Shuzhen Zhang Songjie Feng Wen Jiang Xingxu Huang Jieping Chen Department of Hematology Southwest HospitalThird Military Medical University(Army Medical University)ChongqingChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina

Background:Catalytic defect Cas9‐cytosine deaminase fusion is widely used in base *** Multiple copy numbers of the MS2 binding site(MBS)can recruit multiple MS2 coat proteins(MCPs),which are usually applied to amplify *** study aimed to apply the MS2 signal amplification system to the base editing system in order to achieve simultaneous mutations of multiple bases at the target genome ***:Multiple copy numbers of the MS2 were ligated to the 3′‐end of sgRNA,and MCP was fused to the 5′‐end of cytosine *** MS2 was recognized by MCP to recruit cytosine deaminase for base substitutions(C‐T)at the target *** Cas9 variants,different cytosine deaminases and different copy numbers of MS2 were tested in this system,and the different versions of base editors were compared by editing efficiency and ***:In this study,dCas9,nCas9(D10A)and nCas9(H840A)were *** these 3 Cas9 variants,dCas9 exhibited higher base mutation *** cytosine deaminases were then applied and the efficiency of rAPOBEC1 deaminase was found to be higher than *** also increased the copy numbers of MS2 linked to sgRNA from 2 to ***,the sgRNA‐12x MS2 did not improve the editing efficiency or increase the editing ***:An optimal version of base editor based on the MS2 system,MS2‐BErAPOBEC1(sgRNA‐2x MS2,MCP‐rAPOBEC1 and dCas9),was *** tool can simultaneously mutate multiple bases at the target site,providing a new approach for the study of genome functions.

关键词： base editing functional screening MS2‐MCP simultaneously multiple mutagenesis

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