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silver nanoparticles-decorated and mesoporous silica coated single-walled carbon nanotubes with an enhanced antibacterial activity for killing drug-resistant bacteria

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Nano Research 2020年第2期13卷 389-400页

作者： Yu Zhu Jia Xu Yanmao Wang Cang Chen Hongchen Gu Yimin Chai Yao Wang Shanghai Jiao Tong University Affiliated Sixth Peoples Hospital School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200030China

The mounting threat of antibiotic-resistant bacterial infections has made it imperative to develop innovative antibacterial *** we propose a novel antibacterial nanoplatform of silver nanoparticles-decorated and mesoporous silica coated single-walled carbon nanotubes constructed via a N-[3-(trimethoxysiltyl)propyl]ethylene diamine(TSD)-mediated method(SWCNTs@mSiO2-TSD@Ag).In this system,the outer mesoporous silica shells are able to improve the dispersibility of SWCNTs,which will increase their contact area with bacteria cell ***,the large number of mesopores in silica layers act as microreactors for in situ synthesis of Ag NPs with controlled small size and uniform distribution,which induces an enhanced antibacterial *** with TSD modified mesoporous silica coated single-walled carbon nanotubes(SWCNTs@mSiO2-TSD)and commercialAg NPs,this combination nanosystem of SWCNTs@mSiO2-TSD@Ag exhibits much stronger antibacterial performance against multi-drug-resistant bacteria Escherichia coli(***)and Staphylococcus aureus(***)in vitro through damaging the bacterial cell membranes and a fast release of silver ***,the in vivo rat skin infection model verifies that SWCNTS@mSiO2-TSD@Ag have remarkably improved abilities of bacterial clearance,wound healing promoting as well as outstanding ***,this novel nanoplatform indicates promising potentials as a safe and powerful tool for the treatment of clinical drug-resistant infections.

关键词： silver nanoparticles single-walled carbon nanotubes drug-resistant bacteria enhanced antibacterial activity wound infections

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silver nanoparticles embedded temperature-sensitive nanofibrous membrane as a smart free-standing SERS substrate

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Chinese Chemical Letters 2019年第12期30卷 2021-2026页

作者： Xiaoyun Liu Liying Wang Siyuan Chen Liusheng Zha Research Center for Analysis and Measurement Donghua UniversityShanghai 201620China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials&College of Materials Science and Engineering Donghua UniversityShanghai 201620China

To develop a smart free-standing surface enhanced Raman scattering(SERS) substrate,silver nanoparticles(AgNPs) embedded temperature-sensitive nanofibrous membrane was fabricated by electrospinning their aqueous solution containing the copolymer poly(N-isopropylacrylamide-co-Nhydroxymethylacrylamide),followed by heat treatment to form crosslinking structure within its constituent *** avoid negative effect of the additive like stabilizer and the reactant like reductant on their SERS efficiency,the AgNPs were in-situ synthesized through reducing Ag^+ions dissolved in the polymer solution by ultraviolet *** prepared hybrid nanofibrous membrane with high stability in aqueous medium can reach its swelling or deswelling equilibrium state within 15 seconds with the medium temperature changing between 25℃and 50℃*** it was used as a free-standing SERS substrate,10^-12 mol/L of 4-nitrothiophenol in aqueous solution can be detected at room temperature,and elevating detection temperature can further lower its low detection *** its generated SERS signal has desirable reproducibility,it can be used as SERS substrate for quantitative ***,the hybrid membrane as SERS substrate is capable of real-time monitoring the reduction of 4-nitrothiophenol into 4-aminothiophenol catalyzed by its embedded AgNPs,and the detected intermediate indicates that the reaction proceeds via a condensation route.

关键词： Nanofibrous membrane silver nanoparticles Electrospinning Temperature-sensitiveness SERS substrate

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mRNA transcriptome profiling of human hepatocellular carcinoma cells HepG2 treated with Catharanthus roseus-silver nanoparticles

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World Journal of Hepatology 2023年第3期15卷 393-409页

作者： Nur Asna Azhar Siti Aishah Abu Bakar Marimuthu Citartan Nor Hazwani Ahmad Department of Biomedical Science Advanced Medical and Dental InstituteUniversiti Sains MalaysiaKepala Batas 13200Pulau PinangMalaysia Liver Malignancies Research Program Advanced Medical and Dental InstituteUniversiti Sains MalaysiaKepala Batas 13200Pulau PinangMalaysia Faculty of Bioresources and Food Industry Universiti Sultan Zainal AbidinBesut CampusBesut 22200TerengganuMalaysia

BACKGROUND The demand for the development of cancer nanomedicine has increased due to its great therapeutic value that can overcome the limitations of conventional cancer ***,the presence of various bioactive compounds in crude plant extracts used for the synthesis of silver nanoparticles(AgNPs)makes its precise mechanisms of action *** To assessed the mRNA transcriptome profiling of human HepG2 cells exposed to Catharanthus roseus ***(***)-*** The proliferative activity of hepatocellular carcinoma(HepG2)and normal human liver(THLE3)cells treated with *** were measured using MTT *** RNA samples were extracted and sequenced using BGIseq500 *** is followed by data filtering,mapping,gene expression analysis,differentially expression genes analysis,Gene Ontology analysis,and pathway *** The mean IC 50 values of *** on HepG2 was 4.38±1.59μg/mL while on THLE3 cells was 800±1.55μg/*** profiling revealed an alteration of 296 *** induced the expression of stress-associated genes such as MT,HSP and *** signalling pathways were potentially activated through MAPK,TNF and TGF pathways that are responsible for apoptosis and cell cycle *** alteration of ARF6,EHD2,FGFR3,RhoA,EEA1,VPS28,VPS25,and TSG101 indicated the uptake of ***-AgNPs via both clathrin-dependent and clathrinindependent *** This study provides new insights into gene expression study of biosynthesised AgNPs on cancer *** cytotoxicity effect is mediated by the aberrant gene alteration,and more interestingly the unique selective antiproliferative properties indicate the *** as an ideal anticancer candidate.

关键词： Catharanthus roseus HepG2 silver nanoparticles Transcriptome oxidative stress Apoptosis Cell cycle

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Application of silver nanoparticles in electrically conductive adhesives with silver micro flakes

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China Welding 2022年第2期31卷 23-28页

作者：刘昊马瑞赵丁伟崔志远张微微王建强 Harbin Institute of Technology(Shenzhen) Shenzhen 518054China Academy of Defense Technology China Aerospace Science and Industry Corp.Beijing 100854China

This study has been conducted to evaluate the application of silver nanoparticles(NPs)in Electrically Conductive Adhesives(ECAs),filled with hybrid silver flakes and NPs,and silver flakes as a control sample,at a filler loading of 78 wt.%,83 wt.%and 88 wt.%and cured at 150℃and 180℃,*** results show that the electrical and thermal conductivities of ECAs were improved with the increasing of filler loading and curing *** silver NPs in silver flakes negatively affected the electrical and thermal conductivities of ECAs at a low filler mass fraction of 78 wt.%,because the segregation of NPs enlarged the average distance of silver flakes;while it positively influenced the electrical and thermal conductivities of ECAs at a loading ratio of 88 wt.%,probably due to NPs filling in the gaps between silver flakes or even sintering together with each other or with silver flakes,especially when curing at high temperature of 180℃.

关键词： electrically conductive adhesives silver nanoparticles mass fraction curing temperature

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A Systematic Review on Antituberculosis Drug Discovery and Antimycobacterial Potential of Biologically Synthesized silver nanoparticles:Overview and Future Perspectives

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Infectious Microbes & Diseases 2022年第4期4卷 139-148页

作者： Christian K.Ezeh Chibuzor N.Eze Uju M.E.Dibua Stephen C.Emencheta Department of Microbiology University of NigeriaNsukkaEnugu StateNigeria Department of Pharmaceutical Microbiology University of NigeriaNsukkaEnugu StateNigeria

Rapid emergence and quick evolution of drug-resistant and aggressive mycobacterial strains have resulted in the present antimycobacterial drug crisis and the persistence of tuberculosis as a major public health ***/biological nanotechnologies constitute an interesting area of research for discovering antimycobacterial *** review focused on the biological(green)synthesis of silver nanoparticles(AgNPs)as an alternative source of antimycobacterial *** for this study were searched and screened from three electronic databases(Google Scholar,PubMed and ScienceDirect)following the Preferred Reporting Items for Systematic Reviews and Meta-analyses *** from in total 17 eligible studieswere reported in this systematic *** of the 17 studies used plants to fabricate AgNPs,whereas the remaining five studies used microorganisms(bacteria and/or fungi).silver as part of silver nitrate(AgNO3)was themetal precursor reported for the synthesis of AgNPs in these *** nanoparticles were mostly spherical,with sizes ranging from12 *** minimum inhibitory concentrations varied between studies and were divided into three groups:(i)those more effective than the antibiotic(controls),(ii)those more effective than plant extracts,and(iii)those less effective than the antibiotic *** addition,little or no cytotoxicity effects were *** nanoparticles were also shown to be highly specific or selective toward mycobacterial *** systematic review highlights the antimycobacterial potential of biologically synthesized AgNPs,underscoring the possibility of discovering/developing new antimycobacterial agents using biological synthesis approaches with less toxicity and high selectivity.

关键词： antimycobacterial activity silver nanoparticles cytotoxicity biological synthesis tuberculosis mycobacterial

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SERS activity of carbon nanotubes modified by silver nanoparticles with different particle sizes

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Chinese Physics B 2022年第7期31卷 529-538页

作者：张晓蕾张洁罗元冉佳 Chongqing Municipal Level Key Laboratory of Photoelectronic Information Sensing and Transmitting Technology College of Optoelectronic EngineeringChongqing University of Posts and TelecommunicationsChongqing 400065China Postdoctoral Research Center of Chongqing Key Laboratory of Optoelectronic Information Sensing and Transmission Technology Chongqing University of Posts and TelecommunicationsChongqing 400065China Key Laboratory of Optoelectronic Technology&System Ministry of EducationCollege of Optoelectronic EngineeringChongqing UniversityChongqing 400044China

A two-dimensional(2D)surface-enhanced Raman scattering(SERS)substrate is fabricated by decorating carbon nanotube(CNT)films with Ag nanoparticles(Ag NPs)in different sizes,via simple and low-cost chemical reduction method and self-assembling *** change of Raman and SERS activity of carbon nanotubes/Ag nanoparticles(CNTs/Ag NPs)composites with varying size of Ag NPs are investigated by using rhodamine 6G(R6G)as a probe ***,the scattering cross section of Ag NPs and the distribution of electric field of CNTs/Ag NPs composite are simulated through finite difference time domain(FDTD)*** plasmon resonance(SPR)wavelength is redshifted as the size of Ag NPs increases,and the intensity of SERS and electric field increase with Ag NPs size *** experiment and simulation results show a Raman scattering enhancement factor(EF)of 108for the hybrid substrate.

关键词： Raman spectroscopy surface-enhanced Raman scattering(SERS) carbon nanotubes silver nanoparticles

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Controlled growth of silver nanoparticles in a hydrothermal process

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China Particuology 2007年第3期5卷 206-212页

作者： Juan Zou Yao Xu Bo Hou Dong Wu Yuhan Sun State Key Laboratory of Coal Conversion Institute of Coal Chemistry Chinese Academy of Sciences Taiyuan 030001 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China CAS Key Laboratory of Carbon Materials Institute of Coal Chemistry Chinese Academy of Sciences Taiyuan 030001 China

A two-step synthesis was used to control the shape of silver nanoparticles prepared via reduction of Ag^＋ ions in aqueous Ag（NH3）2NO3 by poly（N-vinyl-2 First, a few spherical silver nanoparticles,-10 nm in size, were pyrrolidone）（PVP）. Then, in a subsequent hydrothermal treatment, the remaining Ag^＋ ions were reduced by PVP into polyhedral nanoparticles, or larger spherical nanoparticles formed from the small spherical seed silver nanoparticles in the first step. The morphology and size of the resultant particles depend on the hydrothermal temperature, PVP/Ag molar ratio and concentration of Ag^＋ ions. By using UV-visible spectroscopy （UV-vis）, transmission electron microscopy （TEM） and powder X-ray diffraction （XRD）, the possible growth mechanism of the silver nanoparticles was discussed. 2007 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V.

关键词： silver nanoparticles Hydrothermal process Morphology-evolvement Nanoplates

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Enviro-Friendly Synthesis of silver nanoparticles Using Berberis lycium Leaf Extract and Their Antibacterial Efficacy

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Acta Metallurgica Sinica(English Letters) 2014年第1期27卷 75-80页

作者： Ansar Mehmood Ghulam Murtaza Tariq Mahmood Bhatti Rehana Kausar Department of Botany University of Azad Jammu and Kashmir (UAJK)Muzaffarabad-13100Pakistan Department of Chemical and Material Engineering Pakistan Institute of Engineering and Applied Sciences(PIEAS)Islamabad-45650Pakistan

In the present study, an enviro-friendly synthesis of silver nanoparticles from Berberis lycium Royle leaf extract and their antibacterial efficacy against five pathogenic bacteria were investigated. This biosynthesis technique is proved to be advantageous over physical and chemical methods as no toxic chemicals are used. The structural and morphological characterization was made by UV-visible spectroscopy, scanning electron microscopy, and transmission electron microscopy. The synthesized nanoparticles were oval, rectangular, and spherical in shape, size ranges from 8 to 100 nm and exhibited an absorption peak at 458 nm. The biosynthesized silver nanoparticles have shown good antibacterial effect toward tested bacteria. It is believed that these biosynthesized silver nanoparticles can play a vital role in nano-based products in future.

关键词： Antibacterial Berberis lycium Enviro-friendly silver nanoparticles

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Comparative study of leaching of silver nanoparticles from fabric and effective effluent treatment

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Journal of Environmental Sciences 2012年第5期24卷 852-859页

作者： Aneesh Pasricha Sant Lal Jangra Nahar Singh Neeraj Dilbaghi K. N. Sood Kanupriya Arora Renu Pasricha Delhi Public School Dwarka New Delh-110078i India Department of Bio & Nano Technology Guru Jambheshwar University of Science & Technology Hisar Haryana-125001 India CSIR-National Physical Laboratory

Nano silver （Agn） is employed as an active antimicrobial agent, but the environmental impact of Agn released from commercial products is unknown. The quantity of nanomaterial released from consumer products during use should be determined to assess the environmental risks of advancement of nanotechnology. This work investigated the amount of silver released from three different types of fabric into water during washing. Three different types of fabric were loaded with chemically synthesized Ag nanoparticles and washed repeatedly under simulated washing conditions. Variable leaching rates among fabric types suggest that the manufacturing process may control the release of silver reaching the waste water treatment plants. In an attempt to recover the Agn for reutilization and to save it from polluting water, the effluents from the wash were efficiently treated with bacterial strains. This treatment was based on biosorption and was very efficient for the elimination of silver nanoparticles in the wash water. The process ensured the recovery of the Agn leached into the effluent for reutilization, thus preventing environmental repercussions.

关键词： silver nanoparticles leaching eco-environment effluent SEM TEM AAS

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Histopathological studies and oxidative stress of synthesized silver nanoparticles in Mozambique tilapia (Oreochromis mossambicus)

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Journal of Environmental Sciences 2012年第6期24卷 1091-1098页

作者： Rajakumar Govindasamy Abdul Abdul Rahuman Unit of Nanotechnology and Bioactive Natural Products Post Graduate and Research Department of ZoologyC.Abdul Hakeem CollegeMelvisharam-632509Vellore DistrictTamil NaduIndia

To evaluate the potential environmental effects of engineered nano metals,it is important to determine the adverse effects of various nanomaterials on aquatic *** tilapia （Oreochromis mossambicus） were maintained in 10 L glass aquaria,and exposed to a graded series of synthesized silver nanoparticles （Ag-NPs） at 25,50 and 75 mg/L for eight *** LC 50 value was 12.6 mg/*** activities of antioxidant enzymes and the contents of antioxidants were lowered in the gills and liver of fishes treated with AgNPs,which resulted in heavy accumulation of free *** results imply that the balance between the oxidative and antioxidant system in the fish was broken down during Ag-NPs *** principal concern related with the release of nanomaterials and their smaller particle may change the materials transport and potential toxicity to aquatic organisms compared to larger particles.

关键词： silver nanoparticles Oreochromis mossambicus oxidative stress analysis histopathological studies toxic effect

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