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Natural Course of silicosis in Dust-exposed Workers

Journal of Huazhong University of Science and Technology(Medical Sciences) 2006年第2期26卷 257-260页

作者：杨海兵杨磊张钧岳陈镜琼 Department of Occupational Health and Environmental Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030China

To provide a scientific basis for determining the health surveillance period of dust-exposed workers, data of a retrospective cohort study was re-analyzed with emphasis on natural course of silicosis. 33640 workers exposed to silica dust who were employed for at least 1 year from 1972 to 1974 in twenty Chinese mines or pottery factories were included as subjects, and were followed up till December 31, 1994. The cohort included subjects from 8 tungsten mines, 4 tin mines and 8 pottery factories. Our results showed that the mean latency of silicosis, for all the cases of the cohorts, was 22.9±9.8 y. 52.2 % of silicosis was diagnosed approximately 9.1±5.7 y after the dust exposure had ceased. The progression rates of silicosis from stage Ⅰ to Ⅱ and from stage Ⅱ to Ⅲ were 48.2 % and 18.5 %, and the duration was 4.1±0. 2 and 6.8±0.2 y, respectively. The survival times of silicosis stage Ⅰ , Ⅱ and Ⅲ, from the year of diagnosis to death, were 21.5, 15.8 and 6.8 years, respectively. There was 25 % of the silicosis patients whose survival time was beyond 33 y. The mean death age of all silicosis cases was 56.0 y. The death age inereased to 65.6 y in the middle of 1990s. Among dust-exposed workers, subjects who became suspected case （0^＋） accounted for 15.0 %. 48.7 % of the suspected silicosis cases developed to silicosis, and the average year from the time of being suspected of the disease to the first stage of silicosis was 5.1y. The natural characteristics, as mentioned above, varied with different mines and factories. We are led to conclude that silicosis is chronic in nature, but progress quickly. As a serious occupational disease it significantly reduces the life span of exposed workers. The study of its natural history is of importance for the development of health surveillance criteria for dust-exposed workers.

关键词： silicosis latency retrospective cohort study health surveillance criteria

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The study of the impact by atractylenolide-1 on inflammatory cytokine,autophagy and apoptosis in alveolar macrophages of silicosis patients

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China Medical Abstracts(Internal Medicine) 2022年第1期39卷 17-18页

作者：谭诗旖 Med Sch Hunan Normal UnivChangsha 410000

Objective To explore the effect of atractylenolide-1( ATL-Ⅰ) on alveolar macrophages in silicosis patients.Methods In December 2019,12 male silicosis patients treated in Beidaihe Sanatorium for Chinese Coal Miners from July to September 2019 were selected by random sampling.Their alveolar macrophages were collected and divided into control group,ATL-Ⅰ group( 100 μmol/L) and dimethyl sulfoxide( DMSO) group( 100 μmol/L).

关键词： patients alveolar silicosis

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Inhibition of gasdermin D-dependent pyroptosis attenuates the progression of silica-induced pulmonary inflammation and fibrosis

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Acta Pharmaceutica Sinica B 2022年第3期12卷 1213-1224页

作者： Meiyue Song Jiaxin Wang Youliang Sun Junling Pang Xiaona Li Yuan Liu Yitian Zhou Peiran Yang Tianhui Fan Ying Liu Zhaoguo Li Xianmei Qi Baicun Li Xinri Zhang Jing Wang Chen Wang State Key Laboratory of Medical Molecular Biology Department of PhysiologyInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing 100730China Tsinghua-Peking Center for Life Sciences Department of BiologyCollege of MedicineTsinghua UniversityBeijing 100084China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China Department of Pulmonary and Critical Care Medicine Center of Respiratory MedicineChina-Japan Friendship HospitalBeijing 100029China National Clinical Research Center for Respiratory Diseases Beijing 100029China Peking Union Medical College Chinese Academy of Medical SciencesBeijing 100730China Peking Union Medical College MD ProgramBeijing 100730China Department of Respiratory the Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086China Department of Pulmonary and Critical Care Medicine the First Hospital of Shanxi Medical UniversityTaiyuan 030001China

silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide,but the molecular basis underlying its development remains unclear.An accumulating body of evidence supports gasdermin D(GSDMD)-mediated pyroptosis as a key component in the development of various pulmonary diseases.However,there is little experimental evidence connecting silicosis and GSDMD-driven pyroptosis.In this work,we investigated the role of GSDMD-mediated pyroptosis in silicosis.Single-cell RNA sequencing of healthy and silicosis human and murine lung tissues indicated that GSDMD-induced pyroptosis in macrophages was relevant to silicosis progression.Through microscopy we then observed morphological alterations of pyroptosis in macrophages treated with silica.Measurement of interleukin-1βrelease,lactic dehydrogenase activity,and real-time propidium iodide staining further revealed that silica induced pyroptosis of macrophages.Additionally,we verified that both canonical(caspase-1-mediated)and non-canonical(caspase-4/5/11-mediated)signaling pathways mediated silica-induced pyroptosis activation,in vivo and in vitro.Notably,Gsdmd knockout mice exhibited dramatically alleviated silicosis phenotypes,which highlighted the pivotal role of pyroptosis in this disease.Taken together,our results demonstrated that macrophages underwent GSDMD-dependent pyroptosis in silicosis and inhibition of this process could serve as a viable clinical strategy for mitigating silicosis.

关键词： silicosis Gasdermin D Pyroptosis Macrophage Pulmonary fibrosis

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Effect of oxidative stress on development of silicosis

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World Journal of Respirology 2012年第1期2卷 1-5页

作者： Rong-Ming Miao Xue-Tao Zhang Ping Guo En-Qi He Fang Zhou Dao-Kun Zhao Ying-Yi Zhang Department of Occupational Disease Wuxi Hospital for Prevention and Treatment of Occupational Disease Department of Occupational Disease Yangpu District Central Hospital Wuxi Center for Disease Control and Prevention

AIM: To investigate the changes of oxidative stress indicators in the serum of silicosis patients and explore the mechanism of silicosis development.METHODS: Two hundred workers who were exposed to silica dust for more than one year were recruited as dust-exposed group, 100 non-dust-exposed subjects served as control group, 32 patients with suspected 0+ silicosis as observation group, and 130 silicosis patients were taken as the silicosis group. Indicators of oxidative stress, including superoxide dismutase(SOD), nitric oxide(NO), serum glutathione peroxidase(GSHPx), total antioxidant capacity(T-AOC), nitric oxide synthase(NOS), and lipid malondialdehyde(MDA), were determined in all the groups. RESULTS: Compared with the control group, NO and GSH-Px in dust-exposed group and silicosis group increased, and SOD decreased significantly(81.162± 35.176, 270.469 ± 39.228 and 68.209 ± 21.528, respectively, P = 0.004, P = 0.002, P = 0.005). Compared with the control and dust-exposed group, T-AOC, NOS and MDA in silicosis group increased significantly(13.048 ± 4.153, 36.201 ± 7.782 and 5.054 ± 1.204, respectively, P = 0.018, P = 0.022, P = 0.011). Compared with dust-exposed group, GSH-Px in the silicosis group increased significantly(270.469 ± 39.228, P = 0.002). GSH-Px in phase Ⅲ silicosis was significantly higher than in phase Ⅰsilicosis(290.750 ± 39.129, P = 0.021). Pearson correlation analysis showed that serum GSH-Px was positively correlated with silicosis staging, length of dust exposure and type of occupation(47.109 ± 8.015, P = 0.001).CONCLUSION: The imbalance of oxidative and antioxidation system is associated with the development of silicosis. The surveillance of oxidative stress indicators will benefit the prognosis of silicosis patients.

关键词： silicosis Oxidative stress Superoxide dismutase Glutathione peroxidase Total antioxidant capacity Nitric oxide synthase Malondialdehyde

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The Effects of N-acetyl-seryl-aspartyl-lysyl-proline on Expression of NF-κb and MCP-1 in Rats with silicosis

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International Journal of Clean Coal and Energy 2013年第2期2卷 24-26页

作者： Qian Li Fang Yang Lijuan Zhang Hong Xu Wenli Zhang Medical research center Hebei United University Thangshan China

In the present study, we developed silicosis of rat model by bronchial perfusion SiO2 dust, and intervenes with AcSDKP, immunohisto chemistry was used to detect NF-κb and MCP-1 expression in lung tissue, and positive cells were counted. We found that compared with silicotic model group, the positive cells of NF-κb and MCP-1 were decreased significantly in anti-fibrosis treatment of AcSDKP group. The findings suggest that AcSDKP could inhibit the expression of NF-κb and MCP-1 in lung tissue of silicosos, this may be related to AcSDKP inhibit of macrophage infiltration in lung tissue and reduced the degree of dust alveolitis.

关键词： N-acetyl-seryl-aspartyl-lysyl-proline silicosis Monocyte Chemotactic Protein-1 Nuclear Factor-κb

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The Technology in Sample Preparation for Transmission Electron Microscopy and Ultrastructure Observation of Lung Tissue in Rats with Experimental silicosis

The Technology in Sample Preparation for Transmission Electr...

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The 2012 International Conference on Advanced Material and Manufacturing Science(ICAMMS 2012)

作者： Qian Li Lijuan Zhang Fang Yang Wenli Zhang Hong Xu Medical research center Hebei United University

It’s hard to get ideal ultrathin sections because of the adamant Si02 dust in silicosis,after perfusion fixation methods and strict control of the cutting speed,improving the success rate of the silicosis tissue TEM sample preparation of ultrathin sections,so we can more clearly and accurately observed ultrastructural changes of silicosis,and it also can offer morphological basis for research the silicosis organizations function histological changes.

关键词： silicosis Transmission Electron Microscopy Ultrathin Section

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Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

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Biomedical and Environmental Sciences 2013年第12期26卷 979-985页

作者： HAO Chang Fu LI Xiao Fang YAO Wu Department of Occupational Health and Occupational Disease College of Public Health Zhengzhou University

Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of a-SMA protein, IGF-liR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin. Results The expression levels of a-SMA concentration and decreased after Wortmann and IGF-IIR increased with the increasing free silica n was used. Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.

关键词： Transdifferentiation Lung fibroblasts Insulin-like growth factor II receptor silicosis

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Protective Effect of Angiotensin(1-7) on Silicotic Fibrosis in Rats

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Biomedical and Environmental Sciences 2019年第6期32卷 419-426页

作者： ZHANG Bo Nan XU Hong GAO Xue Min ZHANG Gui Zhen ZHANG Xin YANG Fang School of Public Health North China University of Science and Technology Tangshan 063210 Hebei China Hebei Key Laboratory for Organ Fibrosis Medical Research Center North China University of Science and Technology Tangshan 063210 Hebei China Hebei Key Laboratory for Chronic Diseases Tangshan Key Laboratory for Clinical and Basic Research on Chronic Diseases School of Basic Medical Sciences North China University of Science and Technology Tangshan 063210 Hebei China Basic Medical College Hebei Medical University Shijiazhuang 050017 Hebei China

Objective silicosis, caused by inhalation of silica dust, is the most serious occupational disease in China and the aim of present study was to explore the protective effect of Ang (1-7) on silicotic fibrosis and myofibroblast differentiation induced by Ang Ⅱ. Methods HOPE-MED 8050 exposure control apparatus was used to establish the rat silicosis model. Pathological changes and collagen deposition of the lung tissue were examined by H.E. and VG staining, respectively. The localizations of ACE2 and a-smooth muscle actin (a-SMA) in the lung were detected by immunohistochemistry. Expression levels of collagen type Ⅰ, a-SMA, ACE2, and Mas in the lung tissue and fibroblasts were examined by western blot. Levels of ACE2, Ang (1-7), and Ang Ⅱ in serum were determined by ELISA. Co-localization of ACE2 and a-SMA in fibroblasts was detected by immunofluorescenee. Results Ang (1-7) induced pathological changes and enhanced collagen deposition in vivo. Ang (1-7) decreased the expressions of collagen type Ⅰ and a-SMA and increased the expressions of ACE2 and Mas in the silicotic rat lung tissue and fibroblasts stimulated by Ang Ⅱ. Ang (1-7) in creased the levels of ACE2 and Ang (1-7) and decreased the level of Ang Ⅱ in silicotic rat serum. A779 enhanced the protective effect of Ang (1-7) in fibroblasts stimulated by Ang Ⅱ. Conclusion Ang (1-7) exerted protective effect on silicotic fibrosis and myofibroblast differentiation induced by Ang Ⅱ by regulating ACE2-Ang (l-7)-Mas axis.

关键词： silicosis Ang Ⅱ Myofibroblasts Ang (1-7) A779

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Dynamic Variation of RAS on Silicotic Fibrosis Pathogenesis in Rats

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Current Medical Science 2019年第4期39卷 551-559页

作者： Bo-nan ZHANG Xin ZHANG Hong XU Xue-min GAO Gui-zhen ZHANG Hui ZHANG Fang YANG School of Public Health Tangshan Key Laboratory for Clinical and Basic Research on Chronic DiseasesSchool of Basic Medical SciencesNorth China University of Science and TechnologyTangshan 063210China Hebei Key Laboratory for Organ Fibrosis Medical Research CenterTangshan Key Laboratory for Clinical and Basic Research on Chronic DiseasesSchool of Basic Medical SciencesNorth China University of Science and TechnologyTangshan 063210China Hebei Key Laboratory for Chronic Diseases Tangshan Key Laboratory for Clinical and Basic Research on Chronic DiseasesSchool of Basic Medical SciencesNorth China University of Science and TechnologyTangshan 063210China Basic Medical College Hebei Medical UniversityShijiazhuang 050017China

The dynamic variation of renin-angiotensin system (RAS) in silicosis remains unclear.Seventy Wistar rats were divided into 7 groups including control group,silicosis groups (inhaling SiO2 for 2,4,8,16 and 24 weeks,respectively) and Captopril (Cap) group.Rat lung primary fibroblasts were divided into control group,SiO2-stimulated group (0,0.5,1,3,6,12,24 and 48 h) and Cap group.The silicotic nodules were formed and collagens were deposited gradually in silicosis group observed by haematoxylin and eosin (HE) staining and Van Gieson (VG) staining.Cap relieved the lung fibrosis and collagen deposition.Immunohistochemistry indicated the positive expression of α-smooth muscle actin α-SMA) was increased gradually in silicotic rat lung tissue.Western blotting revealed the expression of collagen type Ⅰ(Col Ⅰ) and α-SMA was up-regulated in silicotic rat lung tissue and fibroblasts stimulated by SiCh.Cap decreased the expression of Col Ⅰ and α-SMA in silicotic rat lung tissue and fibroblasts stimulated by SiCh.Western blotting also demonstrated the expression of angiotensin-converting enzyme (ACE) and angiotensin Ⅱ type 1 receptor (ATI) was increased,and the expression of ACE2 and Mas was decreased gradually in silicotic rat lung tissue and fibroblasts stimulated by SiCh.ELISA showed the serum levels of ACE and angiotensin Ⅱ(Ang Ⅱ) were also increased and ACE2 and Ang (1 -7) were decreased in the silicosis group.Treatment with Cap decreased the expression levels of ACE,Ang Ⅱ and ATI,and increased the expression levels of ACE2,Ang (1-7) and Mas.These findings suggested that an imbalance between ACE-Ang Ⅱ-AT1 axis and ACE2-Ang (l-7)-Mas axis may participate in the development of silicosis.

关键词： silicosis fibroblasts SiCh renin-angiotensin system α-smooth muscle actin

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The characteristics of plasma lipids in silicosis rat models were studied based on lipid metabolomics

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China Medical Abstracts(Internal Medicine) 2023年第4期40卷 205-205页

作者：何海兰 Sch Public Health North China Sci&Technol UnivTangshan 063210

Objective To screen the differential metabolites and metabolic pathways in silicosis model by analyzing plasma metabolomics of silicosis rats.Methods In May 2021,twenty male SD rats were randomly divided into control group(C),1-week silicosis group(S1W),2-week silicosis group(S2W)and 4-week silicosis group(S4W),with 5 rats in each group.

关键词： silicosis rats lipids

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