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shuxuetong injection protects cerebral microvascular endothelial cells against oxygen-glucose deprivation reperfusion

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Neural Regeneration Research 2019年第5期14卷 783-793页

作者： Zuo-Yan Sun Fu-Jiang Wang Hong Guo Lu Chen Li-Juan Chai Rui-Lin Li Li-Min Hu Hong Wang Shao-Xia Wang Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese Medicine Department of Pharmacy Linyi Central Hospital School of Integrative Medicine Tianjin University of Traditional Chinese Medicine Key Laboratory of Pharmacology of Traditional Chinese Medical Formula Ministry of Education Tianjin University of Traditional Chinese Medicine

shuxuetong injection composed of leech(Hirudo nipponica Whitman) and earthworm(Pheretima aspergillum) has been used for the clinical treatment of acute stroke for many years in China. However, the precise neuroprotective mechanism of shuxuetong injection remains poorly understood. Here, cerebral microvascular endothelial cells(bEnd.3) were incubated in glucose-free Dulbecco's modified Eagle's medium containing 95% N_2/5% CO_2 for 6 hours, followed by high-glucose medium containing 95% O_2 and 5% CO_2 for 18 hours to establish an oxygen-glucose deprivation/reperfusion model. This in vitro cell model was administered shuxuetong injection at 1/32, 1/64, and 1/128 concentrations(diluted 32-, 64-, and 128-times). Cell Counting Kit-8 assay was used to evaluate cell viability. A fluorescence method was used to measure lactate dehydrogenase, and a fluorescence microplate reader used to detect intracellular reactive oxygen species. A fluorescent probe was also used to measure mitochondrial superoxide production. A cell resistance meter was used to measure transepithelial resistance and examine integrity of monolayer cells. The fluorescein isothiocyanate-dextran test was performed to examine blood-brain barrier permeability. Real-time reverse transcription polymerase chain reaction was performed to analyze mRNA expression levels of tumor necrosis factor alpha, interleukin-1β, interleukin-6, and inducible nitric oxide synthase. Western blot assay was performed to analyze expression of caspase-3, intercellular adhesion molecule 1, vascular cell adhesion molecule 1, occludin, vascular endothelial growth factor, cleaved caspase-3, B-cell lymphoma 2, phosphorylated extracellular signal-regulated protein kinase, extracellular signal-regulated protein kinase, nuclear factor-κB p65, I kappa B alpha, phosphorylated I kappa B alpha, I kappa B kinase, phosphorylated I kappa B kinase, claudin-5, and zonula occludens-1. Our results show that shuxuetong injection increases bEnd.3 cell viab

关键词： nerve regeneration shuxuetong injection brain microvascular endothelial cells oxygen-glucose deprivation/reperfusion tight junction proteins mitochondrial function inflammatory factors blood-brain barrier neuroprotection neural regeneration

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The effects of shuxuetong on the pathology of cerebral ischemia-reperfusion injury and GABA and TNF-α expression in gerbil models

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Neuroscience Bulletin 2006年第1期22卷 41-46页

作者： Dong-Jun ZHANG Guang-Run XU Zhi-Yuan LI Yi-Zhao LI Long-Xian XU Feng-Ya LU Qiu-Ling ZHANG Department of Neurology Qilu HospitalShandong University Jinan 250012China Department of Neurology Heze Municipal Hospital Heze 274031 China Department of Physiology Taishan Medical CollegeTaian 271000 China

Objective To study the protective effect and mechanism of shuxuetong on gerbil brain tissue from the area of ischemia-reperfusion. Methods Cerebral ischemia-reperfusion animal model by transient clipping bilateral common carotid arteries in gerbils. Pathological changes in the hippocampal tissue were observed at different reperfusion time （12 h, 3 d, 7 d）. The expression levels of GABA and TNF-α in the hippocampal CA1 subfield were observed using immunohistochemitry at 12 h, 3 d after reperfusion. The difference of above indices among false operation group, ischemia-reperfusion group and treatment group were compared. Results The injuries of pathology to hippocampal area in ischemia-reperfusion group were more serious than treatment group. The expression levels of GABA in treatment group were significantly increased compared with ischemia-reperfusion group, but the expression levels of TNF-α between the two groups have no difference. Conclusion shuxuetong has protective effect on brain tissue of ischemia-reperfusion by enhancing the expression of GABA in the hippocampal tissue.

关键词： shuxuetong ischemia-reperfusion GABA TNF-α

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Combination of shuxuetong and aspirin protects against cerebral ischemia/reperfusion injury through ameliorating coagulation and fibrinolysis system

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Chinese Herbal Medicines 2018年第2期10卷 191-198页

作者： Yong-mei Wu Ya-dong Zhai Yu-ting Zhou Shi-min Tang Xin-yue Li Li-fu Jia Xiang-baoMeng Hui Zhang Gui-bo Sun Xiao-bo Sun Institute of Medicinal Plant Development Peking Union Medical College and Chinese Academy of Medical SciencesZhongguancun Open Laboratory of the Research and Development of Natural Medicine and Health ProductsKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal MedicineMinistry of EducationBeijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine(Natural Medicine)and Translational MedicineBeijing 100193China Department of Pharmacology Changchun University of Chinese MedicineChangchun 130117China North Minzu University Yinchuan 750021China YouBo Pharmaceutical Co. LtdMudanjiang 157013China

Objective：To explore the influence of the combination of shuxuetong（SXT）and aspirin on coagulation and fibrinolytic system of ***：Suture method was applied to establish focal cerebral ischemia-reperfusion injury models in *** rats were randomly divided into sham group,middle cerebral artery occlusion/reperfusion（MCAO/R）group,aspirin group,SXT group,and SXT＋aspirin group（S＆A）.The neurological deficits were assessed according to Longa’s grade 5 scoring *** cerebral edema was detected by measuring the content of water in brain *** volume of cerebral infarction was observed by 2,3,5-Triphenyltetrazolium chloride（TTC）*** plasma was collected by abdominal aortic method to test maximum platelet aggregation rate and four blood ***61,CD62p,6-keto prostaglandin F1αantithrombinⅢ（AT-Ⅲ）,D-dimer,plasminogen activator inhibitor-1（PAI-1）,tissue factor（TF）,tissue plasminogen activator（t-PA）,platelet thromboxaneand von Willebrand factor（v WF）content in rat plasma were detected by ***：SXT combined with aspirin could improve the neurological deficits,alleviate cerebral edema,and decrease the cerebral infarct *** with the sham operation group,fibrinogen（FIB）,6-AT-III,and t-PA in model group were significantly decreased;Compared with the model group,the above-mentioned indexes in SXT and aspirin treatment group were significantly *** prothrombin time（PT）,activated partial thromboplastin time（APTT）,thrombin time（TT）,D-dimer,PAI-1,TF,TXB2,and v WF of the model group were significantly increased;The above-mentioned indexes in blood SXT＋aspirin treated group were significantly *** was a significant difference between the combined group and SXT *** maximum concentration of plateletsin aspirin treated rats was significantly decreased,however,MPAR was reversed in SXT＋aspirin treated ***：SXT combined with aspirin can effectively inhibit platelet activation,regulate the maximum concentra

关键词： aspirin blood coagulation fibrinolysis system shuxuetong

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